Identification of Platinum-Resistance Associated Proteins through Proteomic Analysis of Human Ovarian Cancer Cells and Their Platinum-Resistant Sublines

2007 ◽  
Vol 6 (2) ◽  
pp. 772-780 ◽  
Author(s):  
Xue-dong Yan ◽  
Ling-ya Pan ◽  
Ye Yuan ◽  
Jing-he Lang ◽  
Ning Mao
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Ovarian Cancer Cells ◽  
Platinum Resistance ◽  
Human Ovarian Cancer ◽  
Platinum Resistant ◽  
Associated Proteins

Short-Term Serum Deprivation Confers Sensitivity to Taxanes in Platinum-Resistant Human Ovarian Cancer Cells

2011 ◽  
Vol 21 (9) ◽  
pp. 1547-1554 ◽  
Author(s):  
Seiji Isonishi ◽  
Motoaki Saito ◽  
Misato Saito ◽  
Tadao Tanaka
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Flow Cytometric Analysis ◽  
Serum Deprivation ◽  
Ovarian Cancer Cells ◽  
Enhancement Effect ◽  
Human Ovarian Cancer ◽  
Platinum Resistant ◽  
Mitochondrial Functions ◽  
Resistant Cells

BackgroundBased on the evidences showing that serum deprivation provokes apoptosis in a variety of cells, we have investigated the effect of serum deprivation on drug sensitivity.MethodsAfter human ovarian cancer cells were preincubated in 0.5 % serum containing medium for 12 hours, cellular drug sensitivities were determined by colony-forming assay.ResultsSerum deprivation treatment resulted in significant increase in paclitaxel sensitivity by factors of mean ± SD, 148.6 ± 28.1 and 10.1 ± 1.0 (n = 3;P< 0.001) fold in platinum-resistant C13 and CP70 cells, respectively. Similarly, serum deprivation induced significant docetaxel sensitivity in these cell lines. However, no enhancement effect of serum deprivation was observed in platinum-sensitive 2008 and A2780 cells. Serum deprivation did not have any effect on the sensitivities to cisplatin, vincristin, and doxorubicin in all of these cells. More than 7-fold increase of apoptotic cells were observed in C13 or CP70 cells when they were treated by serum deprivation followed by paclitaxel compared with the treatment of either serum deprivation or paclitaxel alone. Confocal laser microscopy using rhodamine 123 and flow cytometric analysis with 3,3′-dihexyloxacarbocyanine iodide revealed that serum deprivation decreased mitochondrial membrane potential in C13 or CP70 cells, whereas no change was observed in 2008 and A2780 cells. This indicates that serum deprivation induced depolarization specifically in platinum-resistant cells. Electron microscopy revealed that serum deprivation caused regeneration of mitochondrial matrix structure in C13 or CP70 cells where mitochondria were usually destructed and disappeared.DiscussionsThese results indicate that serum deprivation confers taxane hypersensitivity specifically in platinum-resistant cells by recovering their impaired mitochondrial functions. The evidence might be clinically beneficial for the development of new chemotherapeutic technology, particularly for the patients with platinum-resistant ovarian cancer.

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

2011 ◽  
Vol 5 (3-4) ◽  
pp. 199-199
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant

scholarly journals Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel‐resistant sublines

2015 ◽  
Vol 106 (8) ◽  
pp. 1075-1083 ◽  
Author(s):  
Ming Chen ◽  
Hong Huang ◽  
Haojie He ◽  
Wantao Ying ◽  
Xin Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Proteomic Analysis ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Quantitative Proteomic Analysis

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

PROTEOMICS ◽  
2010 ◽  
Vol 10 (21) ◽  
pp. 3789-3799 ◽  
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant

scholarly journals Erratum: Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

PROTEOMICS ◽  
2011 ◽  
Vol 11 (5) ◽  
pp. 1012-1012 ◽  
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant
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