scholarly journals Erratum: Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

PROTEOMICS ◽  
2011 ◽  
Vol 11 (5) ◽  
pp. 1012-1012 ◽  
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

2011 ◽  
Vol 5 (3-4) ◽  
pp. 199-199
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines

PROTEOMICS ◽  
2010 ◽  
Vol 10 (21) ◽  
pp. 3789-3799 ◽  
Author(s):  
Zhiqin Dai ◽  
Jie Yin ◽  
Haojie He ◽  
Wenrui Li ◽  
Chunmei Hou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Comparative Proteomics ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Platinum Resistant

Short-Term Serum Deprivation Confers Sensitivity to Taxanes in Platinum-Resistant Human Ovarian Cancer Cells

2011 ◽  
Vol 21 (9) ◽  
pp. 1547-1554 ◽  
Author(s):  
Seiji Isonishi ◽  
Motoaki Saito ◽  
Misato Saito ◽  
Tadao Tanaka
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Flow Cytometric Analysis ◽  
Serum Deprivation ◽  
Ovarian Cancer Cells ◽  
Enhancement Effect ◽  
Human Ovarian Cancer ◽  
Platinum Resistant ◽  
Mitochondrial Functions ◽  
Resistant Cells

BackgroundBased on the evidences showing that serum deprivation provokes apoptosis in a variety of cells, we have investigated the effect of serum deprivation on drug sensitivity.MethodsAfter human ovarian cancer cells were preincubated in 0.5 % serum containing medium for 12 hours, cellular drug sensitivities were determined by colony-forming assay.ResultsSerum deprivation treatment resulted in significant increase in paclitaxel sensitivity by factors of mean ± SD, 148.6 ± 28.1 and 10.1 ± 1.0 (n = 3;P< 0.001) fold in platinum-resistant C13 and CP70 cells, respectively. Similarly, serum deprivation induced significant docetaxel sensitivity in these cell lines. However, no enhancement effect of serum deprivation was observed in platinum-sensitive 2008 and A2780 cells. Serum deprivation did not have any effect on the sensitivities to cisplatin, vincristin, and doxorubicin in all of these cells. More than 7-fold increase of apoptotic cells were observed in C13 or CP70 cells when they were treated by serum deprivation followed by paclitaxel compared with the treatment of either serum deprivation or paclitaxel alone. Confocal laser microscopy using rhodamine 123 and flow cytometric analysis with 3,3′-dihexyloxacarbocyanine iodide revealed that serum deprivation decreased mitochondrial membrane potential in C13 or CP70 cells, whereas no change was observed in 2008 and A2780 cells. This indicates that serum deprivation induced depolarization specifically in platinum-resistant cells. Electron microscopy revealed that serum deprivation caused regeneration of mitochondrial matrix structure in C13 or CP70 cells where mitochondria were usually destructed and disappeared.DiscussionsThese results indicate that serum deprivation confers taxane hypersensitivity specifically in platinum-resistant cells by recovering their impaired mitochondrial functions. The evidence might be clinically beneficial for the development of new chemotherapeutic technology, particularly for the patients with platinum-resistant ovarian cancer.

Synthetic Naphthoflavonoids Showing Inhibitory Effects on Clonogenicity against Cisplatin-Resistant A2780/Cis Human Ovarian Cancer Cells

2015 ◽  
Vol 12 (8) ◽  
pp. 628-639
Author(s):  
Yearam Jung ◽  
Soon Young Shin ◽  
Yeonjoong Yong ◽  
Hyuk Yoon ◽  
Seunghyun Ahn ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Inhibitory Effects

Gadolinium Oxide Nanoparticles Enhance the Cytotoxicity of Chemotherapeutic Drugs by Blocking Autophagic Flux in Human Ovarian Cancer Cells

2020 ◽  
Vol 16 ◽  
Author(s):  
Zhixiong Xie ◽  
Tianyu Zhang ◽  
Cheng Zhong
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Hela Cells ◽  
Late Stage ◽  
Ovarian Cancer Cells ◽  
Oxide Nanoparticles ◽  
Autophagic Flux ◽  
Human Ovarian Cancer ◽  
Chemotherapeutic Drugs ◽  
Chemotherapy Drugs

Background: During chemotherapy, drugs can damage cancer cells’ DNA and cytomembrane structure, and then induce cell death. However, autophagy can increase the chemotherapy resistance of cancer cells, reducing the effect of chemotherapy. Objective: To block the autophagic flux in cancer cells, it is vital to enhance the anti-cancer efficacy of chemotherapy drugs; for this purpose, we test the gadolinium oxide nanoparticles (Gd2O3 NPs)’ effect on autophagy. Methods: The cytotoxicity of Gd2O3 NPs on HeLa cells was evaluated by a (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, monodasylcadaverine staining, immunofluorescence, immunoblot and apoptosis assay were conducted to evaluate the effect of Gd2O3 NPs on autophagy and efficacy of chemotherapy drugs in human ovarian cancer cells. Results: We found that Gd2O3 NPs, which have great potential for use as a contrast agent in magnetic resonance imaging, could block the late stage of autophagic flux in a dose-dependent manner and then cause autophagosome accumulation in HeLa cells. When co-treated with 8 μg/mL Gd2O3 NPs and 5 μg/mL cisplatin, the number of dead HeLa cells increased by about 20% compared with cisplatin alone. We observed the same phenomenon in cisplatin-resistant COC1/DDP cells. Conclusion: We conclude that Gd2O3 NPs can block the late stage of autophagic flux and enhance the cytotoxicity of chemotherapeutic drugs in human ovarian cancer cells. Thus, the nanoparticles have significant potential for use in both diagnosis and therapy of solid tumor.

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