In Search of Secreted Protein Biomarkers for the Anti-inflammatory Effect of β2-Adrenergic Receptor Agonists:  Application of DIGE Technology in Combination with Multivariate and Univariate Data Analysis Tools

2005 ◽  
Vol 4 (6) ◽  
pp. 2015-2023 ◽  
Author(s):  
Kitty C. M. Verhoeckx ◽  
Marco Gaspari ◽  
Sabina Bijlsma ◽  
Jan van der Greef ◽  
Renger F. Witkamp ◽  
...  
Keyword(s):  
Data Analysis ◽  
Adrenergic Receptor ◽  
Secreted Protein ◽  
Protein Biomarkers ◽  
Receptor Agonists ◽  
Analysis Tools ◽  
Β2 Adrenergic Receptor ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Obesity Affects β2 Adrenergic Regulation of the Inflammatory Profile and Phenotype of Circulating Monocytes from Exercised Animals

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2630 ◽  
Author(s):  
Isabel Gálvez ◽  
Leticia Martín-Cordero ◽  
María Dolores Hinchado ◽  
Alberto Álvarez-Barrientos ◽  
Eduardo Ortega
Keyword(s):  
Inflammatory Response ◽  
Adrenergic Receptor ◽  
Acute Exercise ◽  
Inflammatory Responses ◽  
Adrenergic Regulation ◽  
Immune Stress ◽  
Β2 Adrenergic Receptor ◽  
Anti Inflammatory ◽  
Inflammatory Profile ◽  
Inflammatory Effect

Anomalous immune/inflammatory responses in obesity take place along with alterations in the neuroendocrine responses and dysregulation in the immune/stress feedback mechanisms. Exercise is a potential anti-inflammatory strategy in this context, but the influence of exercise on the β2 adrenergic regulation of the monocyte-mediated inflammatory response in obesity remains completely unknown. The first objective of this study was to analyze the effect of exercise on the inflammatory profile and phenotype of monocytes from obese and lean animals, and the second aim was to determine whether obesity could affect monocytes’ inflammatory response to β2 adrenergic activation in exercised animals. C57BL/6J mice were allocated to different lean or obese groups: sedentary, with acute exercise, or with regular exercise. The inflammatory profile and phenotype of their circulating monocytes were evaluated by flow cytometry in the presence or absence of the selective β2 adrenergic receptor agonist terbutaline. Exercise caused an anti-inflammatory effect in obese individuals and a pro-inflammatory effect in lean individuals. β2 adrenergic receptor stimulation exerted a global pro-inflammatory effect in monocytes from exercised obese animals and an anti-inflammatory effect in monocytes from exercised lean animals. Thus, β2 adrenergic regulation of inflammation in monocytes from exercised animals seems to depend on the inflammatory basal set-point.

The anti-inflammatory effect of A3adenosine receptor agonists: a novel targeted therapy for rheumatoid arthritis

2007 ◽  
Vol 16 (10) ◽  
pp. 1601-1613 ◽  
Author(s):  
Sara Bar-Yehuda ◽  
Michael H Silverman ◽  
William D Kerns ◽  
Avivit Ochaion ◽  
Shira Cohen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Targeted Therapy ◽  
Receptor Agonists ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals MO330ACTIVATION OF Β2 ADRENERGIC RECEPTOR SIGNALING IN MACROPHAGES BLOCKS SYSTEMIC INFLAMMATION AND PROTECTS AGAINST RENAL ISCHEMIA/REPERFUSION INJURY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Sho Hasegawa ◽  
Tsuyoshi Inoue ◽  
Masaomi Nangaku ◽  
Reiko Inagi
Keyword(s):  
Rna Sequencing ◽  
Systemic Inflammation ◽  
Adrenergic Receptor ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Renal Tissue ◽  
Β2 Adrenergic Receptor ◽  
Anti Inflammatory

Abstract Background and Aims The sympathetic nervous system regulates immune cell dynamics. However, the detailed role of sympathetic signaling in inflammatory diseases is still unclear because it varies according to the disease situation and responsible cell types. Here, we focused on sympathetic signaling in macrophages and sought to determine its detailed roles in lipopolysaccharide (LPS)-induced systemic inflammation and renal ischemia/reperfusion injury (IRI). Method In vitro, RAW 264.7 cells and murine peritoneal macrophages were used to determine the effects of β2 adrenergic receptor (Adrb2) signaling on LPS-induced proinflammatory cytokine (tumor necrosis factor-α; TNF-α) production. We also identified the critical gene that mediates the anti-inflammatory effect of Adrb2 signaling by RNA-sequencing. In vivo, we examined the effects of salbutamol (a selective Adrb2 agonist) in LPS-induced systemic inflammation and renal IRI. The involvement of macrophage Adrb2 signaling was confirmed by macrophage-specific Adrb2 conditional knockout (cKO) mice and adoptive transfer of salbutamol-treated macrophages. We also performed single-cell RNA sequencing of renal tissue to analyze the renoprotective role of salbutamol-treated macrophages in detail. Results In vitro, norepinephrine, a sympathetic neurotransmitter, suppressed LPS-induced TNF-α production in macrophages. This anti-inflammatory effect was also induced by salbutamol and reversed by butoxamine (a selective Adrb2 antagonist) in a dose-dependent manner, indicating the importance of Adrb2 in this process. RNA sequencing of these macrophages revealed that T-cell immunoglobulin and mucin-3 (Tim3) expressions were upregulated by the activation of Adrb2 signaling, which partially mediated the anti-inflammatory phenotypic alteration in macrophages. In vivo, salbutamol administration mitigated LPS-induced systemic inflammation and protected against renal IRI; this protection was mitigated in macrophage-specific Adrb2 cKO mice. Adoptive transfer of salbutamol-treated macrophages also protected against renal IRI (Figure 1). Single-cell RNA sequencing revealed that this protection was associated with the accumulation of Tim3-expressing macrophages in the renal tissue. Conclusion The activation of β2 adrenergic receptor signaling in macrophages induces anti-inflammatory phenotypic alterations partially via the induction of Tim3 expressions, which blocks LPS-induced systemic inflammation and protects against renal IRI.

scholarly journals Influence of Obesity and Exercise on β2-Adrenergic-Mediated Anti-Inflammatory Effects in Peritoneal Murine Macrophages

Biomedicines ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 556
Author(s):  
Leticia Martín-Cordero ◽  
Isabel Gálvez ◽  
María Dolores Hinchado ◽  
Eduardo Ortega
Keyword(s):  
Peritoneal Macrophages ◽  
Low Grade ◽  
Adrenergic Stimulation ◽  
Β2 Adrenergic Receptor ◽  
Anti Inflammatory ◽  
Inflammatory Profile ◽  
Adrenergic Activation ◽  
Sedentary Conditions ◽  
Adrenergic Receptor Agonist ◽  
Inflammatory Effect

Obesity is a chronic low-grade inflammatory condition, and β2-adrenergic agonists as well as exercise have been proposed as anti-inflammatory strategies in obesity, so it is critical to accurately determine the effects of β2-adrenergic stimulation, especially when combined with other non-pharmacological therapies. The aim of this investigation was to determine the effect of β2-adrenergic activation on the inflammatory profile and phenotype of macrophages, and whether these effects could be affected by obesity and exercise in this condition. High-fat diet-induced obese and lean C57BL/6J mice were allocated to sedentary or exercised groups. The inflammatory profiles and phenotypes of their peritoneal macrophages were assessed by flow cytometry in the presence or absence of the selective β2-adrenergic receptor agonist terbutaline. β2-adrenergic activation caused global phenotypic anti-inflammatory effects in lean and obese sedentary mice, which were more drastic (also including anti-inflammatory effects on the cytokine profile) in obese animals. In exercised lean and obese animals, this anti-inflammatory effect is weaker and only evident by decreased iNOS and IL-8 expression, without changes in the anti-inflammatory markers. Therefore, β2-adrenergic activation leads to anti-inflammatory effects, but these effects are modulated by obesity in sedentary conditions, as well as by regular exercise; but not by obesity in trained conditions.

Abstract 724: Selective β2-adrenergic receptor agonists inhibit the proliferation of 1321N1 astrocytoma cells

2010 ◽  
Author(s):  
Irving W. Wainer ◽  
Lawrence Toll ◽  
Lucita Jimenez ◽  
Willma Polgar ◽  
Carol Green ◽  
...  
Keyword(s):  
Adrenergic Receptor ◽  
Receptor Agonists ◽  
Astrocytoma Cells ◽  
Β2 Adrenergic Receptor ◽  
1321N1 Astrocytoma

scholarly journals PP2A Activation by β2-Adrenergic Receptor Agonists

2003 ◽  
Vol 278 (25) ◽  
pp. 22555-22562 ◽  
Author(s):  
Christine E. Pullar ◽  
Jin Chen ◽  
R. Rivkah Isseroff
Keyword(s):  
Adrenergic Receptor ◽  
Receptor Agonists ◽  
Β2 Adrenergic Receptor ◽  
Pp2a Activation

scholarly journals β2-Adrenergic Receptor Agonists Inhibit the Proliferation of 1321N1 Astrocytoma Cells

2010 ◽  
Vol 336 (2) ◽  
pp. 524-532 ◽  
Author(s):  
L. Toll ◽  
L. Jimenez ◽  
N. Waleh ◽  
K. Jozwiak ◽  
A.Y.-H. Woo ◽  
...  
Keyword(s):  
Adrenergic Receptor ◽  
Receptor Agonists ◽  
Astrocytoma Cells ◽  
Β2 Adrenergic Receptor ◽  
1321N1 Astrocytoma
Sign in / Sign up

Export Citation Format

Share Document