Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC−MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry

2004 ◽  
Vol 3 (3) ◽  
pp. 644-652 ◽  
Author(s):  
David R. Barnidge ◽  
Marcia K. Goodmanson ◽  
George G. Klee ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Prostate Specific Antigen ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Specific Antigen ◽  
Absolute Quantification ◽  
Protein Cleavage

Comprehensive Quantification of Monolignol-Pathway Enzymes inPopulus trichocarpaby Protein Cleavage Isotope Dilution Mass Spectrometry

2012 ◽  
Vol 11 (6) ◽  
pp. 3390-3404 ◽  
Author(s):  
Christopher M. Shuford ◽  
Quanzi Li ◽  
Ying-Hsuan Sun ◽  
Hsi-Chuan Chen ◽  
Jack Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Protein Cleavage

scholarly journals Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5439-5450 ◽  
Author(s):  
Philip L. Loziuk ◽  
Ronald R. Sederoff ◽  
Vincent L. Chiang ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Relative Abundance ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Protein Quantification ◽  
Protein Cleavage ◽  
Absolute Abundance

Relative abundance values and their associated variability are dynamic and dependent on absolute abundance.

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