Reductive Lithiation of Methyl Substituted Diarylmethylsilanes: Application to Silanediol Peptide Precursors

2011 ◽  
Vol 13 (4) ◽  
pp. 732-735 ◽  
Author(s):  
Dácil Hernández ◽  
Rasmus Mose ◽  
Troels Skrydstrup
Keyword(s):  
Reductive Lithiation ◽  
Peptide Precursors

Processing of peptide precursors

1991 ◽  
Vol 19 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Steven P. Smeekens ◽  
Donald F. Steiner
Keyword(s):  
Peptide Precursors

scholarly journals Comparative Analysis of Chromosome-Encoded Microcins

2006 ◽  
Vol 50 (4) ◽  
pp. 1411-1418 ◽  
Author(s):  
María Eloisa Poey ◽  
María F. Azpiroz ◽  
Magela Laviña
Keyword(s):  
Functional Relationship ◽  
General Scheme ◽  
Type I ◽  
Peptide Antibiotics ◽  
Common Features ◽  
Peptide Precursors ◽  
Immunity Genes ◽  
Genetic Clusters ◽  
Secretion Pathways ◽  
Fourth Member

ABSTRACT Microcins are ribosomally synthesized peptide antibiotics that are produced by enterobacterial strains. Although the first studies concentrated on plasmid-encoded activities, in the last years three chromosome-encoded microcins have been described: H47, E492, and M. Here, a new microcin, I47, is presented as a fourth member of this group. Common features exhibited by chromosome-encoded microcins were searched for. The comparison of the genetic clusters responsible for microcin production revealed a preserved general scheme. The clusters essentially comprise a pair of activity-immunity genes which determine antibiotic specificity and a set of microcin maturation and secretion genes which are invariably present and whose protein products are highly homologous among the different producing strains. A strict functional relationship between the maturation and secretion pathways of microcins H47, I47, and E492 was demonstrated through genetic analyses, which included heterologous complementation assays. The peptide precursors of these microcins share a maturation process which implies the addition of a catecholate siderophore of the salmochelin type. Microcins thus acquire the ability to enter gram-negative cells through the catechol receptors. In addition, they employ a common mode of secretion to reach the external milieu by means of a type I export apparatus. The results presented herein lead us to propose that chromosome-encoded microcins constitute a defined subgroup of peptide antibiotics which are strictly related by their modes of synthesis, secretion, and uptake.

scholarly journals Accelerated Protein Biomarker Discovery from FFPE tissue samples using Single-shot, Short Gradient Microflow SWATH MS

2019 ◽  
Author(s):  
Rui Sun ◽  
Christie Hunter ◽  
Chen Chen ◽  
Weigang Ge ◽  
Nick Morrice ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Biomarker Discovery ◽  
Single Shot ◽  
Protein Biomarkers ◽  
Differential Abundance ◽  
Tissue Samples ◽  
Peptide Precursors ◽  
High Consistency ◽  
Method R ◽  
Acquisition Method

ABSTRACTWe report and evaluated a microflow, single-shot, short gradient SWATH MS method intended to accelerate the discovery and verification of protein biomarkers in clinical specimens. The method uses 15-min gradient microflow-LC peptide separation, an optimized SWATH MS window configuration and OpenSWATH software for data analysis.We applied the method to a cohort 204 of FFPE prostate tissue samples from 58 prostate cancer patients and 10 prostatic hyperplasia patients. Altogether we identified 27,976 proteotypic peptides and 4,043 SwissProt proteins from these 204 samples. Compared to a reference SWATH method with 2-hour gradient the accelerated method consumed only 27% instrument time, quantified 80% proteins and showed reduced batch effects. 3,800 proteins were quantified by both methods in two different instruments with relatively high consistency (r = 0.77). 75 proteins detected by the accelerated method with differential abundance between clinical groups were selected for further validation. A shortlist of 134 selected peptide precursors from the 75 proteins were analyzed using MRM-HR, exhibiting high quantitative consistency with the 15-min SWATH method (r = 0.89) in the same sample set. We further verified the capacity of these 75 proteins in separating benign and malignant tissues (AUC = 0.99) in an independent prostate cancer cohort (n=154).Overall our data show that the single-shot short gradient microflow-LC SWATH MS method achieved about 4-fold acceleration of data acquisition with reduced batch effect and a moderate level of protein attrition compared to a standard SWATH acquisition method. Finally, the results showed comparable ability to separate clinical groups.

ChemInform Abstract: Generation of Ynolates via Reductive Lithiation Using Flow Microreactors.

ChemInform ◽  
2014 ◽  
Vol 45 (33) ◽  
pp. no-no
Author(s):  
Satoshi Umezu ◽  
Toshiya Yoshiiwa ◽  
Manabu Tokeshi ◽  
Mitsuru Shindo
Keyword(s):  
Reductive Lithiation ◽  
Flow Microreactors

scholarly journals Sequence similarity between opioid peptide precursors and DNA-binding proteins

FEBS Letters ◽  
1991 ◽  
Vol 282 (1) ◽  
pp. 175-177 ◽  
Author(s):  
Georgy Ya. Bakalkin ◽  
Dmitry Ponomariev ◽  
Raphik A. Sarkisyan ◽  
Lars Terenius
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Sequence Similarity ◽  
Opioid Peptide ◽  
Dna Binding Proteins ◽  
Peptide Precursors
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