Oligonucleotide Synthesis Involving Deprotection of Amidine-Type Protecting Groups for Nucleobases under Acidic Conditions

2010 ◽  
Vol 12 (11) ◽  
pp. 2496-2499 ◽  
Author(s):  
Akihiro Ohkubo ◽  
Yasukazu Kuwayama ◽  
Yudai Nishino ◽  
Hirosuke Tsunoda ◽  
Kohji Seio ◽  
...  
Keyword(s):  
Protecting Groups ◽  
Oligonucleotide Synthesis ◽  
Acidic Conditions

ChemInform Abstract: Nucleotide Chemistry. Part 16. Amidine Protecting Groups for Oligonucleotide Synthesis.

1986 ◽  
Vol 17 (32) ◽  
Author(s):  
L. J. MCBRIDE ◽  
R. KIERZEK ◽  
S. L. BEAUCAGE ◽  
M. H. CARUTHERS
Keyword(s):  
Protecting Groups ◽  
Oligonucleotide Synthesis

Synthese von immobilisierten Peptidfragmenten an Polystyrol-Polyoxyethylen zur Affinitätschromatographie /Synthesis of Immobilized Peptide Fragments on Polystyrene-Polyoxyethylene for Affinity Chromatography

1987 ◽  
Vol 42 (4) ◽  
pp. 455-460 ◽  
Author(s):  
Ernst Bayer ◽  
Heribert Hellstern ◽  
Heiner Eckstein
Keyword(s):  
Affinity Chromatography ◽  
Organic Solvents ◽  
Peptide Synthesis ◽  
Stationary Phases ◽  
Peptide Bond ◽  
Protecting Groups ◽  
Peptide Fragments ◽  
Solid Supports ◽  
Polystyrene Beads ◽  
Acidic Conditions

Abstract Polystyrene-polyoxyethylene craft copolymers have been used for step-wise peptide synthesis. After completion of synthesis the protecting groups are cleaved under acidic conditions, where the polymer-peptide bond is stable. These gels in comparison to polystyrene peptide gels, show better properties for applications in affinity chromatography as well as synthesis on solid supports, because the advantageous properties of polystyrene beads are combined with the excellent spacer behavior of polyoxyethylene chains (mobility, solvation by water and organic solvents). Peptide gels with polylysine sequences have been synthesized as highly selective stationary phases for the separation of the homologous oligo desoxyribonucleotides (dT)n with n = 1-3. The principal possibilities of these gels for affinity chromatography is demonstrated.

scholarly journals Oligonucleotide synthesis using the 2-(levulinyloxymethyl)-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives.

1998 ◽  
Vol 45 (4) ◽  
pp. 949-976 ◽  
Author(s):  
K Kamaike ◽  
H Takahashi ◽  
K Morohoshi ◽  
N Kataoka ◽  
T Kakinuma ◽  
...  
Keyword(s):  
Comparative Study ◽  
Protecting Groups ◽  
Protecting Group ◽  
Oligonucleotide Synthesis ◽  
Amino Groups ◽  
Initiator Trna ◽  
15N Labeling

A comparative study on the utility of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) and 2-(levulinyloxymethyl)benzoyl (LMBz) protecting groups for the 5'-positions of nucleoside 3'-phosphoramidite derivatives in the oligonucleotide synthesis is presented in terms of the syntheses of TpTpT, TpTpTpT, and UpCpApGpUpUpGpG. In addition we describe the synthesis, using the LMNBz protecting group, of the CpCpA terminus triplet of tRNAs bearing exocyclic amino groups with 15N-labeling, and the trimer Gp[A*]pG containing 2'-O-(beta-D-ribofuranosyl)adenosine ([A*]), the latter of which is found at position 64 in the yeast initiator tRNA(Met).

Sign in / Sign up

Export Citation Format

Share Document