Metabolism of Phenylalanine and Cinnamic Acid in Tobacco Cell Lines With High and Low Yields of Cinnamoyl Putrescines

1982 ◽  
Vol 45 (1) ◽  
pp. 88-93 ◽  
Author(s):  
Jochen Berlin ◽  
Ludger Witte
Keyword(s):  
Cell Lines ◽  
Cinnamic Acid ◽  
Tobacco Cell

scholarly journals Selection of Tobacco Cell Lines Resistant to Photobleaching Herbicides

1999 ◽  
Vol 24 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Mamoru HORIKOSHI ◽  
Takashi HIROOKA
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selection Of

Selection of tobacco cell lines resistant to selenoamino acids

1978 ◽  
Vol 13 (3) ◽  
pp. 219-229 ◽  
Author(s):  
Stuart M. Flashman ◽  
Philip Filner
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Selenoamino Acids ◽  
Selection Of

scholarly journals Aluminum-Induced Rapid Changes in the Microtubular Cytoskeleton of Tobacco Cell Lines

2002 ◽  
Vol 43 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Katerina Schwarzerová ◽  
Sylva Zelenková ◽  
Peter Nick ◽  
Zdeňek Opatrný
Keyword(s):  
Cell Lines ◽  
Tobacco Cell ◽  
Microtubular Cytoskeleton ◽  
Rapid Changes

Microwave-assisted Single Step Cinnamic Acid Derivatization and Evaluation for Cytotoxic Potential

2020 ◽  
Vol 21 (3) ◽  
pp. 236-243
Author(s):  
Sonali Mishra ◽  
Shilpi Singh ◽  
Arif Ali ◽  
Amit C. Gupta ◽  
Karuna Shanker ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cinnamic Acid ◽  
Human Cancer ◽  
Single Step ◽  
Breast Adenocarcinoma ◽  
Microwave Assisted Synthesis ◽  
Hek 293 ◽  
Anticancer Properties ◽  
Microwave Assisted

Background: Phenylpropylene biosynthesis pathway plays a crucial role in the vanillin and their derivative(s) production in the plants. The intermediate of vanillin synthesis i.e. cinnamic acid (CA) is converted into 2-Hydroxy 4-MethoxyBenzaldehyde (HMB) in Decalepis arayalpathra having a number of therapeutic value. Objective : Microwave-assisted modifications in cinnamic acid were planned for potential anticancer properties with better yield and efficiency. The present study also confirms the presence of HMB and its precursor i.e. cinnamic acid in D. arayalpathra tubers. Methods: We used a single step Microwave Assisted Synthesis (MAS) to modify cinnamic acid, and then examined the synthetic and natural cinnamic acid derivatives anticancer potential against six human cancer (K-562, WRL-68, A549, A431, MCF-7, and COLO-201) and two normal (L-132 and HEK-293) cell lines at 2, 10 and 50 µg/ml concentrations. Results: β-bromostyrene and β -nitrostyrene have shown inhibition with IC50 values ranging 0.10-21 µM and 0.03-0.06 µM, respectively to the cancer cell lines. β-bromostyrene was the most potent anticancer derivative of CA with better cellular safety and biocompatibility. Conclusions: The present study of microwave-assisted synthesis demonstrates a single-step modification in cinnamic acid. MAS is a fast, reliable, and robust method. The resultant compounds have shown in-vitro anticancer activity against human lung carcinoma and breast adenocarcinoma.

Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines

1981 ◽  
Vol 36 (3-4) ◽  
pp. 210-214 ◽  
Author(s):  
Jochen Berlin ◽  
Ludger Witte
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cell Cultures ◽  
Phenylalanine Ammonia Lyase ◽  
Shikimic Acid ◽  
Shikimate Pathway ◽  
Tobacco Cell ◽  
Substrate Supply ◽  
Acid Accumulation ◽  
High Yields

To study the flow of carbon through the shikimate pathway in tobacco cell cultures with low and high yields of cinnamoyl putrescines, the cell cultures were treated with glyphosate. In the presence of glyphosate the levels of free shikimic acid were increased more than 300-fold by both cell lines. Despite of a normally 10-fold higher level of cinnamoyl putrescines, the high yielding cell line accumulated only 25% more free shikimic acid than the low yielding cell line. This result together with earlier observations indicated that the increased formation of cinnamoyl putrescines was rather limited by the activity of phenylalanine ammonia lyase than by increased substrate supply caused by alterations in the shikimate pathway

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