Reply to “Comment on ‘Size-Dependent Composition and Molar Extinction Coefficient of PbSe Semiconductor Nanocrystals’”

ACS Nano ◽  
2009 ◽  
Vol 3 (8) ◽  
pp. 2054-2054 ◽  
Author(s):  
Quanqin Dai ◽  
Yingnan Wang ◽  
Xinbi Li ◽  
Yu Zhang ◽  
Donald J. Pellegrino ◽  
...  
Keyword(s):  
Extinction Coefficient ◽  
Semiconductor Nanocrystals ◽  
Molar Extinction Coefficient ◽  
Size Dependent

Size-Dependent Composition and Molar Extinction Coefficient of PbSe Semiconductor Nanocrystals

ACS Nano ◽  
2009 ◽  
Vol 3 (6) ◽  
pp. 1518-1524 ◽  
Author(s):  
Quanqin Dai ◽  
Yingnan Wang ◽  
Xinbi Li ◽  
Yu Zhang ◽  
Donald J. Pellegrino ◽  
...  
Keyword(s):  
Extinction Coefficient ◽  
Semiconductor Nanocrystals ◽  
Molar Extinction Coefficient ◽  
Size Dependent

Comment on “Size-Dependent Composition and Molar Extinction Coefficient of PbSe Semiconductor Nanocrystals”

ACS Nano ◽  
2009 ◽  
Vol 3 (8) ◽  
pp. 2053-2053 ◽  
Author(s):  
Iwan Moreels ◽  
Karel Lambert ◽  
David De Muynck ◽  
Frank Vanhaecke ◽  
Dirk Poelman ◽  
...  
Keyword(s):  
Extinction Coefficient ◽  
Semiconductor Nanocrystals ◽  
Molar Extinction Coefficient ◽  
Size Dependent

Determining the Concentration of CuInS2 Quantum Dots from the Size-Dependent Molar Extinction Coefficient

2012 ◽  
Vol 24 (11) ◽  
pp. 2064-2070 ◽  
Author(s):  
Matthew Booth ◽  
Andrew P. Brown ◽  
Stephen D. Evans ◽  
Kevin Critchley
Keyword(s):  
Quantum Dots ◽  
Extinction Coefficient ◽  
Molar Extinction Coefficient ◽  
Size Dependent ◽  
Cuins2 Quantum Dots

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

scholarly journals Experimental Determination of the Absorption Cross-Section and Molar Extinction Coefficient of Colloidal CdSe Nanoplatelets

2015 ◽  
Vol 119 (47) ◽  
pp. 26768-26775 ◽  
Author(s):  
Aydan Yeltik ◽  
Savas Delikanli ◽  
Murat Olutas ◽  
Yusuf Kelestemur ◽  
Burak Guzelturk ◽  
...  
Keyword(s):  
Experimental Determination ◽  
Cross Section ◽  
Extinction Coefficient ◽  
Absorption Cross Section ◽  
Molar Extinction Coefficient ◽  
Absorption Cross

Size-Dependent Valence and Conduction Band-Edge Energies of Semiconductor Nanocrystals

ACS Nano ◽  
2011 ◽  
Vol 5 (7) ◽  
pp. 5888-5902 ◽  
Author(s):  
Jacek Jasieniak ◽  
Marco Califano ◽  
Scott E. Watkins
Keyword(s):  
Conduction Band ◽  
Semiconductor Nanocrystals ◽  
Band Edge ◽  
Conduction Band Edge ◽  
Size Dependent

Extinction Coefficient of Plasmonic Nickel Sulfide Nanocrystals and Gold-Nickel Sulfide Core-Shell Nanoparticles

2018 ◽  
Vol 233 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Rasmus Himstedt ◽  
Dominik Hinrichs ◽  
Dirk Dorfs
Keyword(s):  
Extinction Coefficient ◽  
Nickel Sulfide ◽  
Molar Extinction Coefficient ◽  
Localized Surface Plasmon ◽  
Core Shell ◽  
Electromagnetic Spectrum ◽  
Shell Nanoparticles ◽  
Extinction Coefficients ◽  
Optical Applications ◽  
Core Shell Nanoparticles

Abstract In the presented work, the molar extinction coefficient of plasmonic heazlewoodite (Ni3S2) nanoparticles and Au-Ni3S2 core-shell nanoparticles is determined for the first time. The results are compared to analogously determined extinction coefficients of pure Au nanocrystals (NCs), which themselves correlate very well with existing literature on the subject. The measured extinction coefficients at the localized surface plasmon resonance (LSPR) maximum wavelength of nickel sulfide particles are similar to the values of equally sized Au NCs. Therefore, considering the lower cost of the heazlewoodite material, it could be a reasonable alternative for optical applications of nanoparticles showing a LSPR in the visible regime of the electromagnetic spectrum. Furthermore, this study shows, that by growing a Ni3S2 shell onto a pure Au nanocrystal a highly tuneable optical material with variable LSPR frequency and molar extinction coefficient is obtained.

Composition and Size-Dependent Extinction Coefficient of Colloidal PbSe Quantum Dots

2007 ◽  
Vol 19 (25) ◽  
pp. 6101-6106 ◽  
Author(s):  
Iwan Moreels ◽  
Karel Lambert ◽  
David De Muynck ◽  
Frank Vanhaecke ◽  
Dirk Poelman ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Extinction Coefficient ◽  
Size Dependent
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