Cellular Internalization and in Vivo Tracking of Thermosensitive Luminescent Micelles Based on Luminescent Lanthanide Chelate

ACS Nano ◽  
2008 ◽  
Vol 2 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Yong-Yong Li ◽  
Han Cheng ◽  
Zhi-Guo Zhang ◽  
Chang Wang ◽  
Jing-Ling Zhu ◽  
...  
Keyword(s):  
Cellular Internalization ◽  
Lanthanide Chelate

scholarly journals Study on the cellular internalization mechanisms and in vivo anti-bone metastasis prostate cancer efficiency of the peptide T7-modified polypeptide nanoparticles

Drug Delivery ◽  
2020 ◽  
Vol 27 (1) ◽  
pp. 161-169
Author(s):  
Yongwei Gu ◽  
Xinmei Chen ◽  
Haiyan Zhang ◽  
Heyi Wang ◽  
Hang Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Cellular Internalization

Augmented tumor accumulation and photothermal ablation using gold nanoparticles with a particular cellular entry orientation

2018 ◽  
Vol 33 (6) ◽  
pp. 660-675
Author(s):  
Gwang Jin Noh ◽  
Hongsuk Park ◽  
Eun Seong Lee
Keyword(s):  
Gold Nanoparticles ◽  
Tumor Cell ◽  
Gold Nanoparticle ◽  
Cellular Internalization ◽  
Tat Peptide ◽  
Peptide Expression ◽  
Cellular Entry ◽  
Tumor Accumulation

Gold nanoparticles with various functionalities have served as potential tools in nanotechnology for tumor ablation. In this work, we seek to design and develop gold nanoparticle with poly(ethylene glycol)-containing dopamine (hereafter termed as AuND), and to synthesize the AuND with one-sided Tat peptide expression (OT@AuND). We demonstrate the tumor cell-targeting ability on the basis of anti-nonspecific cell binding of OT@AuND and determine how the chemically modified gold nanoparticle–based product affects photothermal tumor therapy in vitro and in vivo. The OT@AuND with a particular cellular entry orientation–induced delayed endocytosis, which is advantageous for enhanced permeability and retention effect-based tumor accumulation. This is because the slower cellular interaction of OT@AuND allows it to have the time to be transported to and bind to the tumor site. In tumor cell lines, OT@AuND showed a lower cellular uptake than gold nanoparticles with full-sided Tat peptide expression (FT@AuND) in the early period (after its in vitro and in vivo administration), but the cellular internalization rate of OT@AuND caught up with that of FT@AuND in the late period. Importantly, the delayed cellular internalization feature of OT@AuND resulted in efficient tumor accumulation in tumor-bearing mice, because the time interval provided OT@AuND more chances not to bind to any cells, but to enter tumor cells, leading to selective photothermal tumor ablation. These data suggest that gold nanoparticles with a particular cellular entry orientation can be further explored as a potential photothermal therapeutic agent and as a strategy to treat tumors.

The aspect ratio effect of drug nanocrystals on cellular internalization efficiency, uptake mechanisms, and in vitro and in vivo anticancer efficiencies

Nanoscale ◽  
2015 ◽  
Vol 7 (8) ◽  
pp. 3588-3593 ◽  
Author(s):  
Baishun Tian ◽  
Xiujuan Zhang ◽  
Caitong Yu ◽  
Mengjiao Zhou ◽  
Xiaohong Zhang
Keyword(s):  
Aspect Ratio ◽  
Cellular Internalization ◽  
Ratio Effect ◽  
Uptake Mechanisms

The aspect ratio effect of drug nanocrystals on their in vitro cellular internalizing efficiency and in vivo antitumor efficiency was investigated.

Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery

2015 ◽  
Vol 3 (8) ◽  
pp. 1507-1517 ◽  
Author(s):  
Xiaojun Cai ◽  
Yongyong Li ◽  
Dong Yue ◽  
Qiangying Yi ◽  
Shuo Li ◽  
...  
Keyword(s):  
Schiff Base ◽  
Gene Delivery ◽  
High Performance ◽  
Endosomal Escape ◽  
Cellular Internalization

In the designed polylysine based catiomer the reversible PEGylation was introduced forin vivocirculation and to augment the cellular internalization, while the Schiff-base linked imidazole to accelerate the endosomal escape and facilitate intracellular DNA unpacking and release.

Cellular Internalization of Dissolved Cobalt Ions from Ingested CoFe2O4 Nanoparticles: In Vivo Experimental Evidence

2013 ◽  
Vol 47 (10) ◽  
pp. 5400-5408 ◽  
Author(s):  
Sara Novak ◽  
Damjana Drobne ◽  
Miha Golobič ◽  
Jernej Zupanc ◽  
Tea Romih ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Cellular Internalization ◽  
Cofe2o4 Nanoparticles ◽  
Cobalt Ions

scholarly journals Biodegraded magnetosomes with reduced size and heating power maintain a persistent activity against intracranial U87-Luc mouse GBM tumors

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Edouard Alphandéry ◽  
Ahmed Idbaih ◽  
Clovis Adam ◽  
Jean-Yves Delattre ◽  
Charlotte Schmitt ◽  
...  
Keyword(s):  
Specific Absorption Rate ◽  
Heating Power ◽  
Cellular Internalization ◽  
Persistent Activity ◽  
Before And After ◽  
Anti Tumor Activity ◽  
Nanoparticle Sizes

Abstract Background An important but rarely addressed question in nano-therapy is to know whether bio-degraded nanoparticles with reduced sizes and weakened heating power are able to maintain sufficient anti-tumor activity to fully eradicate a tumor, hence preventing tumor re-growth. To answer it, we studied magnetosomes, which are nanoparticles synthesized by magnetotactic bacteria with sufficiently large sizes (~ 30 nm on average) to enable a follow-up of nanoparticle sizes/heating power variations under two different altering conditions that do not prevent anti-tumor activity, i.e. in vitro cellular internalization and in vivo intra-tumor stay for more than 30 days. Results When magnetosomes are internalized in U87-Luc cells by being incubated with these cells during 24 h in vitro, the dominant magnetosome sizes within the magnetosome size distribution (DMS) and specific absorption rate (SAR) strongly decrease from DMS ~ 40 nm and SAR ~ 1234 W/gFe before internalization to DMS ~ 11 nm and SAR ~ 57 W/gFe after internalization, a behavior that does not prevent internalized magnetosomes to efficiently destroy U87-Luc cell, i.e. the percentage of U87-Luc living cells incubated with magnetosomes decreases by 25% between before and after alternating magnetic field (AMF) application. When 2 µl of a suspension containing 40 µg of magnetosomes are administered to intracranial U87-Luc tumors of 2 mm3 and exposed (or not) to 15 magnetic sessions (MS), each one consisting in 30 min application of an AMF of 27 mT and 198 kHz, DMS and SAR decrease between before and after the 15 MS from ~ 40 nm and ~ 4 W/gFe down to ~ 29 nm and ~ 0 W/gFe. Although the magnetosome heating power is weakened in vivo, i.e. no measurable tumor temperature increase is observed after the sixth MS, anti-tumor activity remains persistent up to the 15th MS, resulting in full tumor disappearance among 50% of treated mice. Conclusion Here, we report sustained magnetosome anti-tumor activity under conditions of significant magnetosome size reduction and complete loss of magnetosome heating power.

Rifampicin-loaded nanotransferosomal gel for treatment of cutaneous leishmaniasis: passive targeting via topical route

Nanomedicine ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 183-203 ◽  
Author(s):  
Samreen Rabia ◽  
Nadra Khaleeq ◽  
Sibgha Batool ◽  
Muhammad Junaid Dar ◽  
Dong Wuk Kim ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Permeation Rate ◽  
Cellular Internalization ◽  
Flow Cytometry Analysis ◽  
Passive Targeting ◽  
The Mean ◽  
Value Flow ◽  
Chitosan Gel ◽  
Macrophage Uptake

Aim: In this study, the targeting of rifampicin (RIF)-loaded nanotransfersomes (NTs) incorporated in chitosan gel for leishmania-infected macrophages via the topical route was investigated. Materials & methods: NTs were prepared through a thin-film hydration process and incorporated into chitosan gel. Results: The mean particle size of the NTs was 190 nm, with 83% encapsulation efficiency. The permeation rate of the NTs was threefold higher than that of the RIF solution. The NTs improved cellular internalization via passive targeting, which was confirmed by macrophage uptake evaluation. A low IC50 value, flow cytometry analysis and in vivo study demonstrated the RIF-loaded NTs enhanced apoptosis and had better antileishmanial effects. Conclusion: RIF-loaded NT gel could be a fitting carrier for the delivery of antileishmanial drugs in cutaneous leishmaniasis.

Correlation of polymeric micelle sizes and their cellular internalization in vitro and tumor targeting in vivo

RSC Advances ◽  
2014 ◽  
Vol 4 (107) ◽  
pp. 62708-62716 ◽  
Author(s):  
F. R. Cheng ◽  
Y. J. Yang ◽  
Y. Liang ◽  
J. Q. Yan ◽  
J. Cao ◽  
...  
Keyword(s):  
Tumor Targeting ◽  
Polymeric Micelle ◽  
Cellular Internalization

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

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