Cellular Internalization of Dissolved Cobalt Ions from Ingested CoFe2O4 Nanoparticles: In Vivo Experimental Evidence

2013 ◽  
Vol 47 (10) ◽  
pp. 5400-5408 ◽  
Author(s):  
Sara Novak ◽  
Damjana Drobne ◽  
Miha Golobič ◽  
Jernej Zupanc ◽  
Tea Romih ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Cellular Internalization ◽  
Cofe2o4 Nanoparticles ◽  
Cobalt Ions

Cellular Internalization and in Vivo Tracking of Thermosensitive Luminescent Micelles Based on Luminescent Lanthanide Chelate

ACS Nano ◽  
2008 ◽  
Vol 2 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Yong-Yong Li ◽  
Han Cheng ◽  
Zhi-Guo Zhang ◽  
Chang Wang ◽  
Jing-Ling Zhu ◽  
...  
Keyword(s):  
Cellular Internalization ◽  
Lanthanide Chelate

scholarly journals Experimental Evidence for Therapeutic Potentials of Propolis

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2528
Author(s):  
Priyanshu Bhargava ◽  
Debajit Mahanta ◽  
Ashish Kaul ◽  
Yoshiyuki Ishida ◽  
Keiji Terao ◽  
...  
Keyword(s):  
New Zealand ◽  
Experimental Evidence ◽  
Antioxidant Properties ◽  
Geographic Location ◽  
Caffeic Acid Phenethyl Ester ◽  
Candidate Drug ◽  
Brazilian Propolis ◽  
Brazilian Green Propolis

Propolis is produced by honeybees from materials collected from plants they visit. It is a resinous material having mixtures of wax and bee enzymes. Propolis is also known as bee glue and used by bees as a building material in their hives, for blocking holes and cracks, repairing the combs and strengthening their thin borders. It has been extensively used since ancient times for different purposes in traditional human healthcare practices. The quality and composition of propolis depend on its geographic location, climatic zone and local flora. The New Zealand and Brazilian green propolis are the two main kinds that have been extensively studied in recent years. Their bioactive components have been found to possess a variety of therapeutic potentials. It was found that Brazilian green propolis improves the cognitive functions of mild cognitive impairments in patients living at high altitude and protects them from neurodegenerative damage through its antioxidant properties. It possesses artepillin C (ARC) as the key component, also known to possess anticancer potential. The New Zealand propolis contains caffeic acid phenethyl ester (CAPE) as the main bioactive with multiple therapeutic potentials. Our lab performed in vitro and in vivo assays on the extracts prepared from New Zealand and Brazilian propolis and their active ingredients. We provided experimental evidence that these extracts possess anticancer, antistress and hypoxia-modulating activities. Furthermore, their conjugation with γCD proved to be more effective. In the present review, we portray the experimental evidence showing that propolis has the potential to be a candidate drug for different ailments and improve the quality of life.

scholarly journals Hypertrophic cardiomyopathy MYH7 mutation R723G alters mRNA secondary structure

2020 ◽  
Vol 52 (1) ◽  
pp. 15-19
Author(s):  
J. Rose ◽  
T. Kraft ◽  
B. Brenner ◽  
J. Montag
Keyword(s):  
Hypertrophic Cardiomyopathy ◽  
Secondary Structure ◽  
Experimental Evidence ◽  
Point Mutation ◽  
Shape Analysis ◽  
Allelic Imbalance ◽  
Mrna Secondary Structure ◽  
Wild Type ◽  
Mrna Structure

Point mutation R723G in the MYH7 gene causes hypertrophic cardiomyopathy (HCM). Heterozygous patients with this mutation exhibit a comparable allelic imbalance of the MYH7 gene. On average 67% of the total MYH7 mRNA are derived from the MYH7R723G-allele and 33% from the MYH7WT allele. Mechanisms underlying mRNA allelic imbalance are largely unknown. We suggest that a different mRNA lifetime of the alleles may cause the allelic drift in R723G patients. A potent regulator of mRNA lifetime is its secondary structure. To test for alterations in the MYH7R723G mRNA structure we used selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) analysis. We show significantly different SHAPE reactivity of wild-type and MYH7R723G RNA, which is in accordance with bioinformatically predicted structures. Thus, we provide the first experimental evidence for mRNA secondary structure alterations by the HCM point mutation. We assume that this may result in a prolonged lifetime of MYH7R723G mRNA in vivo and subsequently in the determined allelic imbalance.

Blue LED treatment of superficial abrasions: in vivo experimental evidence of wound healing improvement

2018 ◽  
Author(s):  
Giada Magni ◽  
Francesca Rossi ◽  
Francesca Tatini ◽  
Riccardo Cicchi ◽  
Lucia Cavigli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Experimental Evidence ◽  
Blue Led

scholarly journals Study on the cellular internalization mechanisms and in vivo anti-bone metastasis prostate cancer efficiency of the peptide T7-modified polypeptide nanoparticles

Drug Delivery ◽  
2020 ◽  
Vol 27 (1) ◽  
pp. 161-169
Author(s):  
Yongwei Gu ◽  
Xinmei Chen ◽  
Haiyan Zhang ◽  
Heyi Wang ◽  
Hang Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Cellular Internalization

Experimental evidence in the in vivo canine for the collapsible tube model of phonation

1991 ◽  
Vol 89 (3) ◽  
pp. 1358-1363 ◽  
Author(s):  
Gerald S. Berke ◽  
David C. Green ◽  
Marshall E. Smith ◽  
David P. Arnstein ◽  
Vincent Honrubia ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Tube Model ◽  
Collapsible Tube

Functional consequences of contrasting properties of α1/α2 systems

1985 ◽  
Vol 68 (s10) ◽  
pp. 93s-97s ◽  
Author(s):  
Pieter B. M. W. M. Timmermans ◽  
Martin J. M. C. Thoolen ◽  
Adriaan De Jonge ◽  
Bob Wilffert ◽  
Pieter A. Van Zwieten
Keyword(s):  
Smooth Muscle ◽  
Experimental Evidence ◽  
Diastolic Pressure ◽  
Previous Treatment ◽  
Pithed Rat ◽  
Pithed Rats ◽  
Functional Consequences ◽  
Adrenoceptor Agonists

Ample experimental evidence supports the existence of two distinct types of vasoconstrictor α-adrenoceptors in vascular smooth muscle at postjunctional sites, showing similarities with α1 and α2-adrenoceptors. Especially in vivo, the characterization of an α2-adrenoceptor mediating an increase in diastolic pressure has been most successful. In this respect the model of the pithed rat has been employed most frequently (for reviews see [1, 2]). At present, the agents cirazoline, (−) phenylephrine, (±)-erythro-methoxamine, (−)-amidephrine, SKF89748, St587 and Sgd 101/75 fulfil the criteria for selective α1-adrenoceptor agonists; their log dose-vasopressor effect curves are virtually unaffected by previous treatment with yohimbine or rauwolscine (selective dose in pithed rats: 1 mg/kg), whereas prazosin (selective dose in pithed rats: 0.1 mg/kg) causes an appreciable parallel rightward displacement. The reverse holds true for the stimulants B-HT 920, B-HT 933, B-HT 958, UK-14304, xylazine, TL-99, M-7 and DP-6,7-ADTN, which all have been classified as preferential agonists of vascular postjunctional α-adrenoceptors.

scholarly journals A Red Wine Vinegar Beverage can Inhibit the Renin-Angiotensin System: Experimental Evidence in Vivo

2005 ◽  
Vol 28 (7) ◽  
pp. 1208-1210 ◽  
Author(s):  
Sachiko Honsho ◽  
Atsushi Sugiyama ◽  
Akira Takahara ◽  
Yoshioki Satoh ◽  
Yuji Nakamura ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Red Wine ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Wine Vinegar

scholarly journals Recapitulating Cross-Species Transmission of Simian Immunodeficiency Virus SIVcpz to Humans by Using Humanized BLT Mice

2016 ◽  
Vol 90 (17) ◽  
pp. 7728-7739 ◽  
Author(s):  
Zhe Yuan ◽  
Guobin Kang ◽  
Fangrui Ma ◽  
Wuxun Lu ◽  
Wenjin Fan ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Transmission Risk ◽  
Crossing Over ◽  
Phylogenetic Distance ◽  
Immunodeficiency Virus ◽  
Blt Mice ◽  
Hiv 1

ABSTRACTThe origins of human immunodeficiency virus type 1 (HIV-1) have been widely accepted to be the consequences of simian immunodeficiency viruses from wild chimpanzees (SIVcpz) crossing over to humans. However, there has not been anyin vivostudy of SIVcpz infection of humans. Also, it remains largely unknown why only specific SIVcpz strains have achieved cross-species transmission and what transmission risk might exist for those SIVcpz strains that have not been found to infect humans. Closing this knowledge gap is essential for better understanding cross-species transmission and predicting the likelihood of additional cross-species transmissions of SIV into humans. Here we show that humanized bone marrow, thymus, and liver (hu-BLT) mice are susceptible to all studied strains of SIVcpz, including the inferred ancestral viruses of pandemic and nonpandemic HIV-1 groups M (SIVcpzMB897) and N (SIVcpzEK505) as well as strains that have not been found in humans (SIVcpzMT145 and SIVcpzBF1167). Importantly, the ability of SIVcpz to cross the interspecies barrier to infect humanized mice correlates with their phylogenetic distance to pandemic HIV-1. We also identified mutations of SIVcpzMB897 (Env G411R and G413R) and SIVcpzBF1167 (Env H280Q and Q380R) at 14 weeks postinoculation. Together, our results have recapitulated the events of SIVcpz cross-species transmission to humans and identified mutations that occurred during the first 16 weeks of infection, providingin vivoexperimental evidence that the origins of HIV-1 are the consequence of SIVcpz crossing over to humans. This study also revealed that SIVcpz viruses whose inferred descendants have not been found in humans still have the potential to cause an HIV-1-like zoonosis.IMPORTANCEIt is believed that the origins of HIV-1 are the consequence of SIV from wild chimpanzees crossing over to humans. However, the origins of HIV-1 have been linked back to only specific SIVcpz strains. There have been no experiments that directly test thein vivocross-species transmissibility of SIVcpz strains to humans. This is the firstin vivostudy of SIVcpz cross-species transmission. With the humanized-BLT mouse model, we have providedin vivoexperimental evidence of multiple SIVcpz strains crossing over to humans and identified several important mutations of divergent SIVcpz strains after long-term replication in human cells. We also found that the cross-species transmission barrier of SIVcpz to humans correlates with their phylogenetic distance to pandemic HIV-1 group M. Importantly, this work provides evidence that SIVcpz viruses, whose inferred descendants have not been found in humans, still have the potential to cause a future HIV-1-like zoonotic outbreak.

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