Hierarchical Assembly of Plasmonic Nanostructures Using Virus Capsid Scaffolds on DNA Origami Templates

ACS Nano ◽  
2014 ◽  
Vol 8 (8) ◽  
pp. 7896-7904 ◽  
Author(s):  
Debin Wang ◽  
Stacy L. Capehart ◽  
Suchetan Pal ◽  
Minghui Liu ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Dna Origami ◽  
Plasmonic Nanostructures ◽  
Virus Capsid ◽  
Hierarchical Assembly

Reconfigurable Plasmonic Nanostructures Controlled by DNA Origami

2020 ◽  
Vol 36 (2) ◽  
pp. 296-300
Author(s):  
Qipeng Long ◽  
Hanyang Yu ◽  
Zhe Li
Keyword(s):  
Dna Origami ◽  
Plasmonic Nanostructures

scholarly journals Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Shelley F. J. Wickham ◽  
Alexander Auer ◽  
Jianghong Min ◽  
Nandhini Ponnuswamy ◽  
Johannes B. Woehrstein ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Super Resolution ◽  
Dna Origami ◽  
Design And Optimization ◽  
Hierarchical Assembly ◽  
Double Helices ◽  
Specialist Groups ◽  
Parallel Arrays ◽  
Super Resolution Microscopy ◽  
Rhombic Lattice

AbstractDNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.

Hierarchical Assembly of DNA Origami Nanostructures Using Coiled-coil Peptides

2019 ◽  
Author(s):  
Alex Buchberger ◽  
Chad Simmons ◽  
Nour Fahmi ◽  
Ronit Freeman ◽  
Nicholas Stephanopoulos
Keyword(s):  
Self Assembly ◽  
Coiled Coil ◽  
Dna Origami ◽  
Hybrid Nanostructures ◽  
Hierarchical Assembly ◽  
Alternating Copolymer ◽  
Self Assembling ◽  
Potential Applications ◽  
Multiple Routes ◽  
Stepwise Assembly

DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with orthogonal DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of self-assembled structures through multiple routes, to form dimers and trimers, an alternating copolymer of two different origami bundles, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.

scholarly journals Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhongchao Zhao ◽  
Joseph Che-Yen Wang ◽  
Mi Zhang ◽  
Nicholas A. Lyktey ◽  
Martin F. Jarrold ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Medical Applications ◽  
Virus Capsid ◽  
Viral Capsids ◽  
Hierarchical Assembly ◽  
Assembly Pathway ◽  
B Virus ◽  
Icosahedral Virus ◽  
Surface Chemistries ◽  
Virus Capsid Protein

AbstractSymmetrical protein complexes are ubiquitous in biology. Many have been re-engineered for chemical and medical applications. Viral capsids and their assembly are frequent platforms for these investigations. A means to create asymmetric capsids may expand applications. Here, starting with homodimeric Hepatitis B Virus capsid protein, we develop a heterodimer, design a hierarchical assembly pathway, and produce asymmetric capsids. In the heterodimer, the two halves have different growth potentials and assemble into hexamers. These preformed hexamers can nucleate co-assembly with other dimers, leading to Janus-like capsids with a small discrete hexamer patch. We can remove the patch specifically and observe asymmetric holey capsids by cryo-EM reconstruction. The resulting hole in the surface can be refilled with fluorescently labeled dimers to regenerate an intact capsid. In this study, we show how an asymmetric subunit can be used to generate an asymmetric particle, creating the potential for a capsid with different surface chemistries.

Transfer of Two-Dimensional Oligonucleotide Patterns onto Stereocontrolled Plasmonic Nanostructures through DNA-Origami-Based Nanoimprinting Lithography

2016 ◽  
Vol 128 (28) ◽  
pp. 8168-8172 ◽  
Author(s):  
Yinan Zhang ◽  
Jie Chao ◽  
Huajie Liu ◽  
Fei Wang ◽  
Shao Su ◽  
...  
Keyword(s):  
Dna Origami ◽  
Plasmonic Nanostructures ◽  
Two Dimensional

scholarly journals Reconfigurable Three-Dimensional Gold Nanorod Plasmonic Nanostructures Organized on DNA Origami Tripod

ACS Nano ◽  
2017 ◽  
Vol 11 (2) ◽  
pp. 1172-1179 ◽  
Author(s):  
Pengfei Zhan ◽  
Palash K. Dutta ◽  
Pengfei Wang ◽  
Gang Song ◽  
Mingjie Dai ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Dna Origami ◽  
Gold Nanorod ◽  
Plasmonic Nanostructures

DNA origami-templated assembly of plasmonic nanostructures with enhanced Raman scattering

2017 ◽  
Vol 29 (1) ◽  
Author(s):  
Meng-Zhen Zhao ◽  
Xu Wang ◽  
Yi-Kang Xing ◽  
Shao-Kang Ren ◽  
Nan Teng ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Dna Origami ◽  
Plasmonic Nanostructures ◽  
Enhanced Raman Scattering

scholarly journals Long- and short-ranged chiral interactions in DNA-assembled plasmonic chains

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kevin Martens ◽  
Felix Binkowski ◽  
Linh Nguyen ◽  
Li Hu ◽  
Alexander O. Govorov ◽  
...  
Keyword(s):  
Resonance Frequency ◽  
Gold Nanorods ◽  
Optical Materials ◽  
Dna Origami ◽  
Plasmonic Nanostructures ◽  
Plasmonic Resonance ◽  
Optical Fields ◽  
Metallic Structures ◽  
Complex Architectures ◽  
The Individual

AbstractCircular dichroism (CD) has long been used to trace chiral molecular states and changes of protein configurations. In recent years, chiral plasmonic nanostructures have shown potential for applications ranging from pathogen sensing to novel optical materials. The plasmonic coupling of the individual elements of such metallic structures is a crucial prerequisite to obtain sizeable CD signals. We here identify and implement various coupling entities—chiral and achiral—to demonstrate chiral transfer over distances close to 100 nm. The coupling is realized by an achiral nanosphere situated between a pair of gold nanorods that are arranged far apart but in a chiral fashion using DNA origami. The transmitter particle causes a strong enhancement of the CD response, the emergence of an additional chiral feature at the resonance frequency of the nanosphere, and a redshift of the longitudinal plasmonic resonance frequency of the nanorods. Matching numerical simulations elucidate the intricate chiral optical fields in complex architectures.

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