Hierarchical Assembly of Super-DNA Origami Based on a Flexible and Covalent-Bound Branched DNA Structure

2021 ◽  
Author(s):  
Yan Li ◽  
Jin Pei ◽  
Xuehe Lu ◽  
Yunfei Jiao ◽  
Fengsong Liu ◽  
...  
Keyword(s):  
Dna Structure ◽  
Dna Origami ◽  
Hierarchical Assembly ◽  
Branched Dna

Recognition and manipulation of branched DNA structure by junction-resolving enzymes 1 1Edited by P. E. Wright

1997 ◽  
Vol 269 (5) ◽  
pp. 647-664 ◽  
Author(s):  
Malcolm F White ◽  
Marie-Josèphe E Giraud-Panis ◽  
J.Richard G Pöhler ◽  
David M.J Lilley
Keyword(s):  
Dna Structure ◽  
Branched Dna

Polymerase Chain Reaction Based Scaffold Preparation for the Production of Thin, Branched DNA Origami Nanostructures of Arbitrary Sizes

Nano Letters ◽  
2009 ◽  
Vol 9 (12) ◽  
pp. 4302-4305 ◽  
Author(s):  
Elisabeth Pound ◽  
Jeffrey R. Ashton ◽  
Héctor A. Becerril ◽  
Adam T. Woolley
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Origami ◽  
Chain Reaction ◽  
Branched Dna ◽  
Polymerase Chain

scholarly journals Geometry of a branched DNA structure in solution.

1989 ◽  
Vol 86 (19) ◽  
pp. 7336-7340 ◽  
Author(s):  
J. P. Cooper ◽  
P. J. Hagerman
Keyword(s):  
Dna Structure ◽  
Branched Dna ◽  
Structure In Solution

scholarly journals Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Shelley F. J. Wickham ◽  
Alexander Auer ◽  
Jianghong Min ◽  
Nandhini Ponnuswamy ◽  
Johannes B. Woehrstein ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Super Resolution ◽  
Dna Origami ◽  
Design And Optimization ◽  
Hierarchical Assembly ◽  
Double Helices ◽  
Specialist Groups ◽  
Parallel Arrays ◽  
Super Resolution Microscopy ◽  
Rhombic Lattice

AbstractDNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.

Hierarchical Assembly of DNA Origami Nanostructures Using Coiled-coil Peptides

2019 ◽  
Author(s):  
Alex Buchberger ◽  
Chad Simmons ◽  
Nour Fahmi ◽  
Ronit Freeman ◽  
Nicholas Stephanopoulos
Keyword(s):  
Self Assembly ◽  
Coiled Coil ◽  
Dna Origami ◽  
Hybrid Nanostructures ◽  
Hierarchical Assembly ◽  
Alternating Copolymer ◽  
Self Assembling ◽  
Potential Applications ◽  
Multiple Routes ◽  
Stepwise Assembly

DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with orthogonal DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of self-assembled structures through multiple routes, to form dimers and trimers, an alternating copolymer of two different origami bundles, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.

Structure of the four-way DNA junction and its interaction with proteins

1995 ◽  
Vol 347 (1319) ◽  
pp. 27-36 ◽  
Keyword(s):  
Catalytic Activity ◽  
Enzyme Activities ◽  
Dna Structure ◽  
Minor Groove ◽  
Strand Exchange ◽  
Included Angle ◽  
Folding Process ◽  
Branched Dna ◽  
Recombination Processes ◽  
Important Intermediate

The four-way DNA junction is an important intermediate in recombination processes; it is, the substrate for different enzyme activities. In solution, the junction adopts a right-handed, antiparallel-stacked X -structure formed by the pairwise coaxial-stacking of helical arms. The stereochemistry is determined by the juxtaposition of grooves and backbones, which is optimal when the smaller included angle is 60°. The antiparallel structure has two distinct sides with major and minor groove-characteristics, respectively. The folding process requires the binding of metal cations, in the absence of which, the junction remains extended without helix-helix stacking. The geometry of the junction can be perturbed by the presence of certain base-base mispairs or phosphodiester discontinuities located at the point of strand exchange. The four-way DNA junction is selectively cleaved by a number of resolving enzymes. In a number of cases, these appear to recognize the minor groove face of the junction and are functionally divisible into activities that recognize and bind the junction, and a catalytic activity. Some possible mechanisms for the recognition of branched DNA structure are discussed.

Hierarchical assembly of metal nanoparticles, quantum dots and organic dyes using DNA origami scaffolds

2013 ◽  
Vol 9 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Robert Schreiber ◽  
Jaekwon Do ◽  
Eva-Maria Roller ◽  
Tao Zhang ◽  
Verena J. Schüller ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Metal Nanoparticles ◽  
Organic Dyes ◽  
Dna Origami ◽  
Hierarchical Assembly

Hierarchical Assembly of Plasmonic Nanostructures Using Virus Capsid Scaffolds on DNA Origami Templates

ACS Nano ◽  
2014 ◽  
Vol 8 (8) ◽  
pp. 7896-7904 ◽  
Author(s):  
Debin Wang ◽  
Stacy L. Capehart ◽  
Suchetan Pal ◽  
Minghui Liu ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Dna Origami ◽  
Plasmonic Nanostructures ◽  
Virus Capsid ◽  
Hierarchical Assembly
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