scholarly journals Multicolor Fluorescence Nanoscopy by Photobleaching: Concept, Verification, and Its Application To Resolve Selective Storage of Proteins in Platelets

ACS Nano ◽  
2014 ◽  
Vol 8 (5) ◽  
pp. 4358-4365 ◽  
Author(s):  
Daniel Rönnlund ◽  
Lei Xu ◽  
Anna Perols ◽  
Annica K. B. Gad ◽  
Amelie Eriksson Karlström ◽  
...  
Keyword(s):  
Fluorescence Nanoscopy ◽  
Multicolor Fluorescence ◽  
Selective Storage

scholarly journals Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength

2010 ◽  
Vol 99 (8) ◽  
pp. 2686-2694 ◽  
Author(s):  
Ilaria Testa ◽  
Christian A. Wurm ◽  
Rebecca Medda ◽  
Ellen Rothermel ◽  
Claas von Middendorf ◽  
...  
Keyword(s):  
Living Cells ◽  
Fluorescence Nanoscopy ◽  
Multicolor Fluorescence

scholarly journals Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging

2021 ◽  
Author(s):  
Do-Hyeon Kim ◽  
Yeonho Chang ◽  
Soyeon Park ◽  
Min Gyu Jeong ◽  
Yonghoon Kwon ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Image Artifacts ◽  
Multicolor Fluorescence

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit blue-conversion phenomenon, which can produce severe multicolor image artifacts...

Eliminating Nonspecific Binding Sites for Highly Reliable Immunoassay via Super-resolution Multicolor Fluorescence Colocalization

Nanoscale ◽  
2021 ◽  
Author(s):  
Shenfei Zong ◽  
Yun Liu ◽  
Kuo Yang ◽  
Zhaoyan Yang ◽  
Zhuyuan Wang ◽  
...  
Keyword(s):  
Binding Sites ◽  
Super Resolution ◽  
Specific Adsorption ◽  
Nonspecific Binding ◽  
Nonspecific Adsorption ◽  
Multicolor Fluorescence

Non-specific adsorption in immunoassays has always been a major problem that affects the reliability of assay results. Despite the emergence of various methods which can reduce nonspecific adsorption, a universal...

A Locked Nucleic Acid-Based Nanocrawler: Designed and Reversible Movement Detected by Multicolor Fluorescence

2013 ◽  
Vol 135 (7) ◽  
pp. 2423-2426 ◽  
Author(s):  
I. Kira Astakhova ◽  
Karol Pasternak ◽  
Meghan A. Campbell ◽  
Pankaj Gupta ◽  
Jesper Wengel
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Multicolor Fluorescence

scholarly journals SECURE DEPENDABLE SELECTIVE STORAGE SERVICES AND SUPPORT FOR DYNAMIC DATA OPERATIONS IN CLOUD COMPUTING

2013 ◽  
pp. 35-40
Author(s):  
VINITHA S P ◽  
GURUPRASAD E
Keyword(s):  
Cloud Computing ◽  
Service Providers ◽  
Distributed Storage ◽  
Large Data ◽  
Cloud Service ◽  
Dynamic Data ◽  
Cloud Data ◽  
Data Auditing ◽  
Cloud Servers ◽  
Selective Storage

Cloud computing has been envisioned as the next generation architecture of IT enterprise. It moves the application software and databases to the centralized large data centers where management of data and services may not be fully trustworthy. This unique paradigm brings out many new security challenges like, maintaining correctness and integrity of data in cloud. Integrity of cloud data may be lost due to unauthorized access, modification or deletion of data. Lacking of availability of data may be due to the cloud service providers (CSP), in order to increase their margin of profit by reducing the cost, CSP may discard rarely accessed data without detecting in timely fashion. To overcome above issues, flexible distributed storage, token utilizing, signature creations used to ensure integrity of data, auditing mechanism used assists in maintaining the correctness of data and also locating, identifying of server where exactly the data has been corrupted and also dependability and availability of data achieved through distributed storage of data in cloud. Further in order to ensure authorized access to cloud data a admin module has been proposed in our previous conference paper, which prevents unauthorized users from accessing data and also selective storage scheme based on different parameters of cloud servers proposed in previous paper, in order to provide efficient storage of data in the cloud. In order to provide more efficiency in this paper dynamic data operations are supported such as updating, deletion and addition of data.

scholarly journals Snapshot multicolor fluorescence imaging using double multiplexing of excitation and emission on a single detector

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karolina Dorozynska ◽  
Simon Ek ◽  
Vassily Kornienko ◽  
David Andersson ◽  
Alexandra Andersson ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Fast Imaging ◽  
Imaging Method ◽  
Emission Characteristics ◽  
Cross Term ◽  
Current Implementation ◽  
Visible Wavelength Range ◽  
Imaging Approach ◽  
Single Detector ◽  
Multicolor Fluorescence

AbstractFluorescence-based multispectral imaging of rapidly moving or dynamic samples requires both fast two-dimensional data acquisition as well as sufficient spectral sensitivity for species separation. As the number of fluorophores in the experiment increases, meeting both these requirements becomes technically challenging. Although several solutions for fast imaging of multiple fluorophores exist, they all have one main restriction; they rely solely on spectrally resolving either the excitation- or the emission characteristics of the fluorophores. This inability directly limits how many fluorophores existing methods can simultaneously distinguish. Here we present a snapshot multispectral imaging approach that not only senses the excitation and emission characteristics of the probed fluorophores but also all cross term combinations of excitation and emission. To the best of the authors’ knowledge, this is the only snapshot multispectral imaging method that has this ability, allowing us to even sense and differentiate between light of equal wavelengths emitted from the same fluorescing species but where the signal components stem from different excitation sources. The current implementation of the technique allows us to simultaneously gather 24 different spectral images on a single detector, from which we demonstrate the ability to visualize and distinguish up to nine fluorophores within the visible wavelength range.

scholarly journals ID: 1038 Hydrothermal synthesis of carbon nanodots from millets for cancer cells imaging

2017 ◽  
Vol 4 (S) ◽  
pp. 116
Author(s):  
Quang Ngo Khoa ◽  
Tran Thi Xuan Thuy ◽  
Che Thi Cam Ha
Keyword(s):  
Hydrothermal Synthesis ◽  
Cancer Cells ◽  
Biomedical Application ◽  
Average Diameter ◽  
Optical Measurements ◽  
Carbon Nanodots ◽  
Excitation Wavelength ◽  
Economical Method ◽  
Potential Applications ◽  
Multicolor Fluorescence

We presented a green, simple and economical method to synthesize carbon nanodots (C-dots) from millets using hydrothermal synthesis route. The obtained C-dots have average diameter ranging from 6 to 8 nm. Optical measurements showed the formation of hydroxyl, carbonyl/carboxyl, amino functional groups on the particle surfaces, resulting in their high hydrophilicity and bioconjugation. After treatment with C-dots, human cervical and lung cancer cells became bright and exhibited multicolor fluorescence under different excitation wavelength. The achievement demonstrated potential applications of fluorescent C-dots in the field of biomedical application, especially in diagnostic disease techniques.

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