DNA Origami Nanoneedles on Freestanding Lipid Membranes as a Tool To Observe Isotropic–Nematic Transition in Two Dimensions

Nano Letters ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 649-655 ◽  
Author(s):  
Aleksander Czogalla ◽  
Dominik J. Kauert ◽  
Ralf Seidel ◽  
Petra Schwille ◽  
Eugene P. Petrov
Keyword(s):  
Lipid Membranes ◽  
Two Dimensions ◽  
Dna Origami

scholarly journals Reversible membrane deformations by straight DNA origami filaments

Soft Matter ◽  
2021 ◽  
Author(s):  
Henri G. Franquelim ◽  
Hendrik Dietz ◽  
Petra Schwille
Keyword(s):  
Lipid Membranes ◽  
Dna Origami ◽  
Membrane Bound ◽  
Membrane Deformations

Reversible MgCl2-induced blunt-end polymerization of membrane-bound straight DNA origami monomers into filaments leads to protruding deformations on freestanding lipid membranes.

scholarly journals Diffusion and Freezing Transition of Rod-Like DNA Origami on Freestanding Lipid Membranes

2014 ◽  
Vol 106 (2) ◽  
pp. 701a
Author(s):  
Eugene P. Petrov ◽  
Aleksander Czogalla ◽  
Dominik J. Kauert ◽  
Ralf Seidel ◽  
Petra Schwille
Keyword(s):  
Lipid Membranes ◽  
Dna Origami ◽  
Freezing Transition

scholarly journals Reconstitution of ultrawide DNA origami pores in liposomes for transmembrane transport of macromolecules

2021 ◽  
Author(s):  
Alessio Fragasso ◽  
Nicola De Franceschi ◽  
Pierre Stömmer ◽  
Eli O. van der Sluis ◽  
Hendrik Dietz ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Cell Biology ◽  
Lipid Membrane ◽  
Molecular Transport ◽  
Dna Origami ◽  
Influx Rate ◽  
Membrane Pores ◽  
Synthetic Cells ◽  
Folded Proteins ◽  
Membrane Spanning

AbstractMolecular traffic across lipid membranes is a vital process in cell biology that involves specialized biological pores with a great variety of pore diameters, from fractions of a nanometer to >30 nm. Creating artificial membrane pores covering similar size and complexity will aid the understanding of transmembrane molecular transport in cells, while artificial pores are also a necessary ingredient for synthetic cells. Here, we report the construction of DNA origami nanopores that have an inner diameter as large as 30 nm. We developed new methods to successfully insert these ultrawide pores into the lipid membrane of giant unilamellar vesicles (GUVs) by administering the pores concomitantly with vesicle formation in an inverted-emulsion cDICE technique. The reconstituted pores permit the transmembrane diffusion of large macromolecules such as folded proteins, which demonstrates the formation of large membrane-spanning open pores. The pores are size selective as dextran molecules with a diameter up to 22 nm can traverse the pores, whereas larger dextran molecules are blocked. By FRAP measurements and modelling of the GFP influx rate, we find that up to hundreds of pores can be functionally reconstituted into a single GUV. Our technique bears great potential for applications across different fields from biomimetics, synthetic biology, to drug delivery.

Advancing Biophysics Using DNA Origami

2021 ◽  
Vol 50 (1) ◽  
Author(s):  
Wouter Engelen ◽  
Hendrik Dietz
Keyword(s):  
Electron Microscopy ◽  
Lipid Membranes ◽  
Force Measurement ◽  
Experimental Methods ◽  
Dna Origami ◽  
Added Value ◽  
Annual Review ◽  
Publication Date ◽  
Complex Environments ◽  
Positioning Devices

DNA origami enables the bottom-up construction of chemically addressable, nanoscale objects with user-defined shapes and tailored functionalities. As such, not only can DNA origami objects be used to improve existing experimental methods in biophysics, but they also open up completely new avenues of exploration. In this review, we discuss basic biophysical concepts that are relevant for prospective DNA origami users. We summarize biochemical strategies for interfacing DNA origami with biomolecules of interest. We describe various applications of DNA origami, emphasizing the added value or new biophysical insights that can be generated: rulers and positioning devices, force measurement and force application devices, alignment supports for structural analysis for biomolecules in cryogenic electron microscopy and nuclear magnetic resonance, probes for manipulating and interacting with lipid membranes, and programmable nanopores. We conclude with some thoughts on so-far little explored opportunities for using DNA origami in more complex environments such as the cell or even organisms. Expected final online publication date for the Annual Review of Biophysics, Volume 50 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Dislocation theory of dimer melting in two dimensions: Lipid membranes

1985 ◽  
Vol 31 (1) ◽  
pp. 420-429 ◽  
Author(s):  
A. Holz ◽  
D. T. Vigren ◽  
M. Zuckermann
Keyword(s):  
Lipid Membranes ◽  
Two Dimensions ◽  
Dislocation Theory

Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes

2018 ◽  
Vol 5 (14) ◽  
pp. 1800437 ◽  
Author(s):  
Yusuke Sato ◽  
Masayuki Endo ◽  
Masamune Morita ◽  
Masahiro Takinoue ◽  
Hiroshi Sugiyama ◽  
...  
Keyword(s):  
Self Assembly ◽  
Lipid Membranes ◽  
Dna Origami

The tangled web of agency

2018 ◽  
Vol 41 ◽  
Author(s):  
Alain Pe-Curto ◽  
Julien A. Deonna ◽  
David Sander
Keyword(s):  
Two Dimensions ◽  
Bad Company

AbstractWe characterize Doris's anti-reflectivist, collaborativist, valuational theory along two dimensions. The first dimension is socialentanglement, according to which cognition, agency, and selves are socially embedded. The second dimension isdisentanglement, the valuational element of the theory that licenses the anchoring of agency and responsibility in distinct actors. We then present an issue for the account: theproblem of bad company.

Redox Reactions in Lipid Membranes as a Model for Primordial Energy-Conserving Systems

1997 ◽  
Vol 161 ◽  
pp. 437-442
Author(s):  
Salvatore Di Bernardo ◽  
Romana Fato ◽  
Giorgio Lenaz
Keyword(s):  
Experimental Evidence ◽  
Lipid Membranes ◽  
Energy Supply ◽  
Redox Reactions ◽  
Living Systems ◽  
Asymmetric Distribution ◽  
Conservation Of Energy ◽  
Asymmetrical Distribution ◽  
Energy Conserving ◽  
Living Organisms

AbstractOne of the peculiar aspects of living systems is the production and conservation of energy. This aspect is provided by specialized organelles, such as the mitochondria and chloroplasts, in developed living organisms. In primordial systems lacking specialized enzymatic complexes the energy supply was probably bound to the generation and maintenance of an asymmetric distribution of charged molecules in compartmentalized systems. On the basis of experimental evidence, we suggest that lipophilic quinones were involved in the generation of this asymmetrical distribution of charges through vectorial redox reactions across lipid membranes.

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

Direct visualization of phase-separated domains in lipid membranes

1978 ◽  
Vol 36 (2) ◽  
pp. 290-291
Author(s):  
S. W. Hui ◽  
T. P. Stewart
Keyword(s):  
Lipid Membranes ◽  
Structural Information ◽  
Freeze Fracture ◽  
Biological Molecules ◽  
Vacuum Drying ◽  
Direct Visualization ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
X Ray ◽  
Hydrocarbon Chains

Direct electron microscopic study of biological molecules has been hampered by such factors as radiation damage, lack of contrast and vacuum drying. In certain cases, however, the difficulties may be overcome by using redundent structural information from repeating units and by various specimen preservation methods. With bilayers of phospholipids in which both the solid and fluid phases co-exist, the ordering of the hydrocarbon chains may be utilized to form diffraction contrast images. Domains of different molecular packings may be recgnizable by placing properly chosen filters in the diffraction plane. These domains would correspond to those observed by freeze fracture, if certain distinctive undulating patterns are associated with certain molecular packing, as suggested by X-ray diffraction studies. By using an environmental stage, we were able to directly observe these domains in bilayers of mixed phospholipids at various temperatures at which their phases change from misible to inmissible states.

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