Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes

2018 ◽  
Vol 5 (14) ◽  
pp. 1800437 ◽  
Author(s):  
Yusuke Sato ◽  
Masayuki Endo ◽  
Masamune Morita ◽  
Masahiro Takinoue ◽  
Hiroshi Sugiyama ◽  
...  
Keyword(s):  
Self Assembly ◽  
Lipid Membranes ◽  
Dna Origami

scholarly journals Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek
Keyword(s):  
Electrochemical Properties ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Membrane Resistance ◽  
Electrochemical Characteristics ◽  
Freeze Thaw ◽  
Lipid Films ◽  
Supported Lipid Membranes

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

scholarly journals Co-self-assembly of multiple DNA origami nanostructures in a single pot

2021 ◽  
Author(s):  
Joshua A. Johnson ◽  
Vasiliki Kolliopoulos ◽  
Carlos E. Castro
Keyword(s):  
Self Assembly ◽  
Dna Origami ◽  
Folding Pathways

We demonstrate co-self-assembly of two distinct DNA origami structures with a common scaffold strand through programmable bifurcation of folding pathways.

scholarly journals A Structurally Variable Hinged Tetrahedron Framework from DNA Origami

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
David M. Smith ◽  
Verena Schüller ◽  
Carsten Forthmann ◽  
Robert Schreiber ◽  
Philip Tinnefeld ◽  
...  
Keyword(s):  
Self Assembly ◽  
Gel Electrophoresis ◽  
Imaging Techniques ◽  
Building Blocks ◽  
Dna Origami ◽  
Microscopy Technique ◽  
Wire Frame ◽  
Wide Range ◽  
Potential Applications ◽  
Linker Molecules

Nanometer-sized polyhedral wire-frame objects hold a wide range of potential applications both as structural scaffolds as well as a basis for synthetic nanocontainers. The utilization of DNA as basic building blocks for such structures allows the exploitation of bottom-up self-assembly in order to achieve molecular programmability through the pairing of complementary bases. In this work, we report on a hollow but rigid tetrahedron framework of 75 nm strut length constructed with the DNA origami method. Flexible hinges at each of their four joints provide a means for structural variability of the object. Through the opening of gaps along the struts, four variants can be created as confirmed by both gel electrophoresis and direct imaging techniques. The intrinsic site addressability provided by this technique allows the unique targeted attachment of dye and/or linker molecules at any point on the structure's surface, which we prove through the superresolution fluorescence microscopy technique DNA PAINT.

Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates

2009 ◽  
Vol 5 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Hareem T. Maune ◽  
Si-ping Han ◽  
Robert D. Barish ◽  
Marc Bockrath ◽  
William A. Goddard III ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Self Assembly ◽  
Dna Origami ◽  
Two Dimensional

scholarly journals Modelling DNA origami self-assembly at the domain level

2015 ◽  
Vol 143 (16) ◽  
pp. 165102 ◽  
Author(s):  
Frits Dannenberg ◽  
Katherine E. Dunn ◽  
Jonathan Bath ◽  
Marta Kwiatkowska ◽  
Andrew J. Turberfield ◽  
...  
Keyword(s):  
Self Assembly ◽  
Dna Origami ◽  
Domain Level

Self-Healing Bilayer Lipid Membranes Formed Over Synthetic Substrates

2008 ◽  
Author(s):  
M. Austin Creasy ◽  
Donald J. Leo
Keyword(s):  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Unique Property ◽  
Bilayer Lipid Membranes ◽  
Self Healing ◽  
Electrical Field ◽  
Bilayer Lipid ◽  
Recent Experimental Study

Biological systems demonstrate autonomous healing of damage and are an inspiration for developing self-healing materials. Our recent experimental study has demonstrated that a bilayer lipid membrane (BLM), also called a black lipid membrane, has the ability to self-heal after mechanical failure. These molecules have a unique property that they spontaneously self assembly into organized structures in an aqueous medium. The BLM forms an impervious barrier to ions and fluid between two volumes and strength of the barrier is dependent on the pressure and electrical field applied to the membrane. A BLM formed over an aperture on a silicon substrate is shown to self-heal for 5 pressurization failure cycles.

scholarly journals Self-assembly of highly ordered DNA origami lattices at solid-liquid interfaces by controlling cation binding and exchange

Nano Research ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 3142-3150 ◽  
Author(s):  
Yang Xin ◽  
Salvador Martinez Rivadeneira ◽  
Guido Grundmeier ◽  
Mario Castro ◽  
Adrian Keller
Keyword(s):  
Correlation Length ◽  
Self Assembly ◽  
High Speed ◽  
Time Course ◽  
Divalent Cations ◽  
Topological Analysis ◽  
Monovalent Cation ◽  
Cation Binding ◽  
Dna Origami ◽  
Lattice Order

Abstract The surface-assisted hierarchical self-assembly of DNA origami lattices represents a versatile and straightforward method for the organization of functional nanoscale objects such as proteins and nanoparticles. Here, we demonstrate that controlling the binding and exchange of different monovalent and divalent cation species at the DNA-mica interface enables the self-assembly of highly ordered DNA origami lattices on mica surfaces. The development of lattice quality and order is quantified by a detailed topological analysis of high-speed atomic force microscopy (HS-AFM) images. We find that lattice formation and quality strongly depend on the monovalent cation species. Na+ is more effective than Li+ and K+ in facilitating the assembly of high-quality DNA origami lattices, because it is replacing the divalent cations at their binding sites in the DNA backbone more efficiently. With regard to divalent cations, Ca2+ can be displaced more easily from the backbone phosphates than Mg2+ and is thus superior in guiding lattice assembly. By independently adjusting incubation time, DNA origami concentration, and cation species, we thus obtain a highly ordered DNA origami lattice with an unprecedented normalized correlation length of 8.2. Beyond the correlation length, we use computer vision algorithms to compute the time course of different topological observables that, overall, demonstrate that replacing MgCl2 by CaCl2 enables the synthesis of DNA origami lattices with drastically increased lattice order.

Use DNA origami as a scaffold for self-assembly of optical metamolecules

2015 ◽  
Author(s):  
Yoon Jo Hwang ◽  
Shelley F. J. Wickham ◽  
Steven D. Perrault ◽  
Sanghyun Yoo ◽  
Sung Ha Park ◽  
...  
Keyword(s):  
Self Assembly ◽  
Dna Origami

Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65684-65689 ◽  
Author(s):  
Fan Xu ◽  
Meimei Bao ◽  
Longfei Rui ◽  
Jiaojiao Liu ◽  
Jingliang Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Surface Coating ◽  
Lipid Membrane ◽  
Coating Film ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Self Assembled

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.

DNA Origami Guided Self-Assembly of Plasmonic Polymers with Robust Long-Range Plasmonic Resonance

Nano Letters ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 8926-8932
Author(s):  
Pengfei Wang ◽  
Ji-Hyeok Huh ◽  
Haedong Park ◽  
Donglei Yang ◽  
Yingwei Zhang ◽  
...  
Keyword(s):  
Long Range ◽  
Self Assembly ◽  
Dna Origami ◽  
Plasmonic Resonance
Sign in / Sign up

Export Citation Format

Share Document