Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells

Nano Letters ◽  
2007 ◽  
Vol 7 (4) ◽  
pp. 874-879 ◽  
Author(s):  
Jordan J. Green ◽  
Eugene Chiu ◽  
Elizaveta S. Leshchiner ◽  
Julie Shi ◽  
Robert Langer ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Specific Gene ◽  
Primary Cells ◽  
Human Primary Cells

Multi-component Polymeric System for Tumour Cell-Specific Gene Delivery Using a Universal Bungarotoxin Linker

2010 ◽  
Vol 27 (11) ◽  
pp. 2274-2282 ◽  
Author(s):  
Ralph A. Willemsen ◽  
Michal Pechar ◽  
Robert C. Carlisle ◽  
Erik Schooten ◽  
Robert Pola ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Tumour Cell ◽  
Specific Gene ◽  
Polymeric System

scholarly journals Light-induced adenovirus gene transfer, an efficient and specific gene delivery technology for cancer gene therapy

2002 ◽  
Vol 9 (4) ◽  
pp. 365-371 ◽  
Author(s):  
Anders Høgset ◽  
Birgit Øvstebø Engesæter ◽  
Lina Prasmickaite ◽  
Kristian Berg ◽  
Øystein Fodstad ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Cancer Gene Therapy ◽  
Specific Gene ◽  
Cancer Gene ◽  
Delivery Technology

scholarly journals Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain

2015 ◽  
Vol 112 (22) ◽  
pp. E2947-E2956 ◽  
Author(s):  
Makoto Matsuyama ◽  
Yohei Ohashi ◽  
Tadashi Tsubota ◽  
Masae Yaguchi ◽  
Shigeki Kato ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Cross Reactivity ◽  
Vector System ◽  
Target Cells ◽  
Specific Gene ◽  
Multiple Target ◽  
Neuronal Systems ◽  
Avian Sarcoma

Pathway-specific gene delivery is requisite for understanding complex neuronal systems in which neurons that project to different target regions are locally intermingled. However, conventional genetic tools cannot achieve simultaneous, independent gene delivery into multiple target cells with high efficiency and low cross-reactivity. In this study, we systematically screened all receptor–envelope pairs resulting from the combination of four avian sarcoma leukosis virus (ASLV) envelopes (EnvA, EnvB, EnvC, and EnvE) and five engineered avian-derived receptors (TVA950, TVBS3, TVC, TVBT, and DR-46TVB) in vitro. Four of the 20 pairs exhibited both high infection rates (TVA–EnvA, 99.6%; TVBS3–EnvB, 97.7%; TVC–EnvC, 98.2%; and DR-46TVB–EnvE, 98.8%) and low cross-reactivity (<2.5%). Next, we tested these four receptor–envelope pairs in vivo in a pathway-specific gene-transfer method. Neurons projecting into a limited somatosensory area were labeled with each receptor by retrograde gene transfer. Three of the four pairs exhibited selective transduction into thalamocortical neurons expressing the paired receptor (>98%), with no observed cross-reaction. Finally, by expressing three receptor types in a single animal, we achieved pathway-specific, differential fluorescent labeling of three thalamic neuronal populations, each projecting into different somatosensory areas. Thus, we identified three orthogonal pairs from the list of ASLV subgroups and established a new vector system that provides a simultaneous, independent, and highly specific genetic tool for transferring genes into multiple target cells in vivo. Our approach is broadly applicable to pathway-specific labeling and functional analysis of diverse neuronal systems.

The infection of human primary cells and cell lines by human cytomegalovirus: New tropism and new reservoirs for HCMV

2008 ◽  
Vol 131 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Wei Wang ◽  
Ping Yu ◽  
Peng Zhang ◽  
Yujun Shi ◽  
Hong Bu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Primary Cells ◽  
Human Primary Cells

Tumor-specific gene delivery using RNA-targeting Tetrahymena group I intron

2005 ◽  
Vol 27 (8) ◽  
pp. 567-574 ◽  
Author(s):  
Heung-Su Jung ◽  
Byung-Su Kwon ◽  
Seong-Wook Lee
Keyword(s):  
Gene Delivery ◽  
Group I Intron ◽  
Specific Gene ◽  
Group I ◽  
Rna Targeting
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