Intracellular Delivery of Recombinant Arginine Deiminase (rADI) by Heparin-Binding Hemagglutinin Adhesion Peptide Restores Sensitivity in rADI-Resistant Cancer Cells

2014 ◽  
Vol 11 (8) ◽  
pp. 2777-2786 ◽  
Author(s):  
Fe-Lin Lin Wu ◽  
Tzyy-Harn Yeh ◽  
Ying-Luen Chen ◽  
Yu-Chin Chiu ◽  
Ju-Chen Cheng ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Intracellular Delivery ◽  
Arginine Deiminase ◽  
Heparin Binding

Lipo-Poly(L-histidine) Hybrid Materials with pH-Sensitivity, Intracellular Delivery Efficiency, and Intrinsic Targetability to Cancer Cells

2014 ◽  
Vol 35 (9) ◽  
pp. 888-894 ◽  
Author(s):  
Renjith P. Johnson ◽  
Young-Il Jeong ◽  
Johnson V. John ◽  
Chung-Wook Chung ◽  
Seon Hee Choi ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Hybrid Materials ◽  
Intracellular Delivery ◽  
Ph Sensitivity ◽  
Delivery Efficiency

Engineered Histidine-Enriched Facial Lipopeptides for Enhanced Intracellular Delivery of Functional siRNA to Triple Negative Breast Cancer Cells

2019 ◽  
Vol 11 (5) ◽  
pp. 4719-4736 ◽  
Author(s):  
Abhijit Biswas ◽  
Kasturee Chakraborty ◽  
Chiranjit Dutta ◽  
Sanchita Mukherjee ◽  
Paramita Gayen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Intracellular Delivery

scholarly journals Novel human-derived cell-penetrating peptides for specific subcellular delivery of therapeutic biomolecules

2005 ◽  
Vol 390 (2) ◽  
pp. 407-418 ◽  
Author(s):  
Catherine de Coupade ◽  
Antonio Fittipaldi ◽  
Vanessa Chagnas ◽  
Matthieu Michel ◽  
Sophie Carlier ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mammalian Cells ◽  
Heparan Sulphate ◽  
Intracellular Delivery ◽  
Membrane Lipid ◽  
Cell Penetrating Peptides ◽  
Heparin Binding ◽  
Independent Manner ◽  
Cell Penetrating

Short peptide sequences that are able to transport molecules across the cell membrane have been developed as tools for intracellular delivery of therapeutic molecules. This work describes a novel family of cell-penetrating peptides named Vectocell® peptides [also termed DPVs (Diatos peptide vectors)]. These peptides, originating from human heparin binding proteins and/or anti-DNA antibodies, once conjugated to a therapeutic molecule, can deliver the molecule to either the cytoplasm or the nucleus of mammalian cells. Vectocell® peptides can drive intracellular delivery of molecules of varying molecular mass, including full-length active immunoglobulins, with efficiency often greater than that of the well-characterized cell-penetrating peptide Tat. The internalization of Vectocell® peptides has been demonstrated to occur in both adherent and suspension cell lines as well as in primary cells through an energy-dependent endocytosis process, involving cell-membrane lipid rafts. This endocytosis occurs after binding of the cell-penetrating peptides to extracellular heparan sulphate proteoglycans, except for one particular peptide (DPV1047) that partially originates from an anti-DNA antibody and is internalized in a caveolar independent manner. These new therapeutic tools are currently being developed for intracellular delivery of a number of active molecules and their potentiality for in vivo transduction investigated.

pH- and GSH-Sensitive Hyaluronic Acid-MP Conjugate Micelles for Intracellular Delivery of Doxorubicin to Colon Cancer Cells and Cancer Stem Cells

2018 ◽  
Vol 19 (9) ◽  
pp. 3725-3737 ◽  
Author(s):  
Tilahun Ayane Debele ◽  
Lu-Yi Yu ◽  
Cheng-Sheng Yang ◽  
Yao-An Shen ◽  
Chun-Liang Lo
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Hyaluronic Acid ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Intracellular Delivery ◽  
Colon Cancer Cells

A Facile Approach for Synthesis and Intracellular Delivery of Size Tunable Cationic Peptide Functionalized Gold Nanohybrids in Cancer Cells

2018 ◽  
Vol 29 (4) ◽  
pp. 1102-1110 ◽  
Author(s):  
Kavita Bansal ◽  
Mohammad Aqdas ◽  
Munish Kumar ◽  
Rajni Bala ◽  
Sanpreet Singh ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Intracellular Delivery ◽  
Cationic Peptide

scholarly journals Intracellular delivery of NF-κB small interfering RNA for modulating therapeutic activities of classical anti-cancer drugs in human cervical cancer cells

2013 ◽  
Vol 3 (1) ◽  
pp. 7 ◽  
Author(s):  
Anthony Stanislaus ◽  
Anil Philip Kunnath ◽  
Snigdha Tiash ◽  
Tahereh Fatemian ◽  
Nur Izyani Kamaruzman ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Intracellular Delivery ◽  
Cyclin B1 ◽  
Carbonate Apatite ◽  
Cancer Drugs ◽  
Small Interfering Rnas ◽  
Strong Electrostatic Interaction ◽  
Anti Cancer ◽  
Gene Transcripts

Cervical cancer is the second most common cancer and fourth leading cause of cancer-related deaths among women. Advanced stage of the disease is treated with radiation therapy and chemotherapy with poor therapeutic outcome and adverse side effects. NFκB, a well-known transcription factor in the control of immunity and inflammation, has recently emerged as a key regulator of cell survival through induction of antiapoptotic genes. Many human cancers, including cervical carcinoma, constitutively express NF-κB and a blockade in expression of its subunit proteins through targeted knockdown of the gene transcripts with small interfering RNAs (siRNA) could be an attractive approach in order to sensitize the cancer cells towards the widely used anti-cancer drugs. However, the inefficiency of the naked siRNA to cross the plasma membrane and its sensitiveness to nuclease-mediated degradation are the major challenges limiting the siRNA technology in therapeutic intervention. pH-sensitive carbonate apatite has been established as an efficient nano-carrier for intracellular delivery of siRNA, due to its strong electrostatic interaction with the siRNA, the desirable size distribution of the resulting siRNA complex for effective endocytosis and the ability of the endocytosed siRNA to be released from the degradable particles and escape the endosomes, thus leading to the effective knockdown of the target gene of cyclin B1 or ABCB1. Here, we report that carbonate apatite-facilitated delivery of the siRNA targeting NF-κB1 and NF-κB2 gene transcripts in HeLa, a human cervical adenocar- cinoma cell line expressing NF-κB, led to a synergistic effect in enhancement of chemosensitivity to doxorubicin, but apparently not to cisplatin or paclitaxel.

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