Hexanoic Acid and Polyethylene Glycol Double Grafted Amphiphilic Chitosan for Enhanced Gene Delivery: Influence of Hydrophobic and Hydrophilic Substitution Degree

2014 ◽  
Vol 11 (3) ◽  
pp. 982-994 ◽  
Author(s):  
Buddhadev Layek ◽  
Manas K. Haldar ◽  
Gitanjali Sharma ◽  
Lindsey Lipp ◽  
Sanku Mallik ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Gene Delivery ◽  
Hexanoic Acid ◽  
Substitution Degree

scholarly journals In silico study of PEI-PEG-squalene-dsDNA polyplex formation: The delicate role of PEG length to the binding of PEI to DNA

2021 ◽  
Author(s):  
Tudor Vasiliu ◽  
Bogdan Florin Florin Craciun ◽  
Andrei Neamtu ◽  
Lilia Clima ◽  
Dragos Lucian Isac ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Gene Delivery ◽  
In Silico ◽  
Biomedical Applications ◽  
Experimental Studies ◽  
Biomedical Devices ◽  
In Silico Study ◽  
Or Gene

The biocompatible hydrophilic polyethylene glycol (PEG) is widely used in biomedical applications, such as drug or gene delivery, tissue engineering or as antifouling in biomedical devices. Experimental studies have shown...

Polyethylene glycol–grafted polyethylenimine used to enhance adenovirus gene delivery

2012 ◽  
Vol 101A (7) ◽  
pp. 1857-1864 ◽  
Author(s):  
Kumar Singarapu ◽  
Ivy Pal ◽  
Joshua D. Ramsey
Keyword(s):  
Polyethylene Glycol ◽  
Gene Delivery

Early entry and deformation of macropinosomes correlates with high efficiency of decaarginine-polyethylene glycol-lipid-mediated gene delivery

2012 ◽  
Vol 14 (4) ◽  
pp. 262-271 ◽  
Author(s):  
Shouhei Kobayashi ◽  
Yoshiyuki Hattori ◽  
Hiroko Osakada ◽  
Kazunori Toma ◽  
Yoshie Maitani ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Gene Delivery ◽  
High Efficiency ◽  
Early Entry

Polyethylene Glycol and Polyethylenimine Dual-Functionalized Nano-Graphene Oxide for Photothermally Enhanced Gene Delivery

Small ◽  
2013 ◽  
Vol 9 (11) ◽  
pp. 1989-1997 ◽  
Author(s):  
Liangzhu Feng ◽  
Xianzhu Yang ◽  
Xiaoze Shi ◽  
Xiaofang Tan ◽  
Rui Peng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Polyethylene Glycol ◽  
Gene Delivery ◽  
Nano Graphene

scholarly journals Gene delivery ability of polyethylenimine and polyethylene glycol dual-functionalized nanographene oxide in 11 different cell lines

2017 ◽  
Vol 4 (10) ◽  
pp. 170822 ◽  
Author(s):  
Liping Wu ◽  
Jinshan Xie ◽  
Tan Li ◽  
Zihao Mai ◽  
Lu Wang ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Gene Delivery ◽  
Cell Lines ◽  
High Efficiency ◽  
Articular Chondrocytes ◽  
Transfection Efficiency ◽  
H9c2 Cell ◽  
Hela Cell Lines ◽  
High Efficient ◽  
Plasmid Transfection

We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG−nrGO−PEI, RGPP) for high-efficient gene delivery in HepG2 and Hela cell lines. To evaluate the feasibility and applicability of RGPP as a gene delivery carrier, we here assessed the transfection efficiency of RGPP on gene plasmids and siRNA in 11 different cell lines. Commercial polyalkyleneimine cation transfection reagent (TR) was used as comparison. In HepG2 cells, RGPP exhibited much stronger delivery ability for siRNA and large size plasmids than TR. For green fluorescent protein (GFP) plasmid, RGPP showed about 47.1% of transfection efficiency in primary rabbit articular chondrocytes, and about 27% of transfection efficiency in both SH-SY5Y and A549 cell lines. RGPP exhibited about 37.2% of GFP plasmid transfection efficiency in EMT6 cells and about 26.0% of GFP plasmid transfection efficiency in LO2 cells, but induced about 33% of cytotoxicity in both cell lines. In 4T1 and H9C2 cell lines, RGPP had less than 10% of GFP plasmid transfection efficiency. Collectively, RGPP is a potential nano-carrier for high-efficiency gene delivery, and needs to be further optimized for different cell lines.

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