Nonviral gene delivery using poly-D /L aspartate-diethylenetriamine cationic polymers and polyethylene glycol: A two-step approach

2011 ◽  
Vol 50 (5) ◽  
pp. 836-850 ◽  
Author(s):  
Janni Mirosevich ◽  
Gregoire Cardoen ◽  
Brian Burke ◽  
Tara Costich ◽  
Kevin Sill
Keyword(s):  
Polyethylene Glycol ◽  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Cationic Polymers

Nonviral Vehicles for Gene Delivery

Nano LIFE ◽  
2021 ◽  
Vol 11 (02) ◽  
pp. 2130002
Author(s):  
Eric Warga ◽  
Brian Austin-Carter ◽  
Noelle Comolli ◽  
Jacob Elmer
Keyword(s):  
Gene Delivery ◽  
Lower Cost ◽  
Clinical Applications ◽  
Inorganic Nanoparticles ◽  
Nonviral Gene Delivery ◽  
Viral Gene ◽  
Cationic Polymers ◽  
Recent Developments ◽  
Low Efficiency ◽  
Viral Gene Delivery

Nonviral gene delivery (NVGD) is an appealing alternative to viral gene delivery for clinical applications due to its lower cost and increased safety. A variety of promising nonviral vectors are under development, including cationic polymers, lipids, lipid-polymer hybrids (LPHs) and inorganic nanoparticles. However, some NVGD strategies have disadvantages that have limited their adoption, including high toxicity and low efficiency. This review focuses on the most common NVGD vehicles with an emphasis on recent developments in the field.

Gene Expression Profiles in Mouse Lung Tissue after Administration of Two Cationic Polymers Used for Nonviral Gene Delivery

2006 ◽  
Vol 23 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Karin Regnström ◽  
Eva G. E. Ragnarsson ◽  
Mårten Fryknäs ◽  
Magnus Köping-Höggård ◽  
Per Artursson
Keyword(s):  
Gene Expression ◽  
Gene Delivery ◽  
Lung Tissue ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Nonviral Gene Delivery ◽  
Mouse Lung ◽  
Cationic Polymers ◽  
Mouse Lung Tissue

Abstract 599: Development of poly(Asp-DET) cationic polymers for nonviral gene delivery

2010 ◽  
Author(s):  
Janni Mirosevich ◽  
Gregoire Cardoen ◽  
Kevin Sill ◽  
Brian Burke
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Cationic Polymers

Polyplex: A Promising Gene Delivery System

2019 ◽  
Vol 12 (6) ◽  
pp. 4681-4686
Author(s):  
Rohan Aggarwal ◽  
Monika Targhotra ◽  
Bhumika Kumar ◽  
P.K Sahoo ◽  
Meenakshi K Chauhan
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Gene Delivery System ◽  
Cationic Polymers ◽  
Therapeutic Application ◽  
Promising Alternative ◽  
Structure And Stability ◽  
The Past

In the past few years gene delivery system has gained a huge attention owing to its proved efficacy in several diseases especially in those caused by genetic and/oroncological malfunctioning. The effective gene delivery mainly depends on the carrier molecules that can ensure the safe and specific delivery of the nucleic acidmolecules. Viral vectors have been used for a longer period as the gene transfer vehicle. However, these viral vectors have potential immunological disadvantages that made them less preferred. Recently, non-viral vectors such as polyplexes have emerged as a promising alternative for viral vectors. Polyplexes are formed by conjugating a polymer with DNA and in maximum cases the cationic polymers are preferred over others. The structure and stability of the polyplexes depends on various factors. The ability of the polymer to condense the DNA mainly dictates the efficiency of the polyplex mediated transfection. In this review we are going to provide a framework for the synthesis and design of the polyplexes along with the structure and stability of the complexes pertaining to mechanism of action, characterization and therapeutic application, including polyethyleneimine mediated cytotoxicity as well as newer strategies for the generation of better polyplexes.

scholarly journals Glucose-Responsive Gene Delivery at Physiological pH through Tertiary-Amine Stabilized Boronate-PVA Particles Synthesized by One-Pot Reaction

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 62
Author(s):  
Mangesh Morey ◽  
Akshay Srivastava ◽  
Abhay Pandit
Keyword(s):  
Gene Delivery ◽  
Fold Increase ◽  
Nonviral Gene Delivery ◽  
Luciferase Reporter ◽  
Hydrodynamic Diameter ◽  
Luciferase Reporter Gene ◽  
One Pot ◽  
Gaussia Luciferase ◽  
Nih3t3 Cells ◽  
The Stability

We report a physiologically stable and cytocompatible glucose-responsive nonviral gene delivery system made up of boronate functionalized polymeric material. Herein, we utilize boronate cis-diol interactions to develop a glucose-responsive submicron particle (SMP) system. The stability of the boronate interaction at a physiological pH was achieved by copolymerization of dimethyl aminoethyl methacrylate (DMAEMA) with acrylamidophenylboronic acid (AAPBA) and the formation of a complex with polyvinylalcohol (PVA) which is governed by cis-diol interactions. The shift in hydrodynamic diameter of SMPs was observed and correlated with increasing glucose concentrations at a physiological pH. Optimal transfection was observed for a 5 µg dose of the gaussia luciferase reporter gene in NIH3T3 cells without any adverse effect on cellular viability. The destabilization of the AAPBA–PVA complex by interacting with glucose allowed the release of encapsulated bovine serum albumin (BSA) in a glucose-responsive manner. In total, 95% of BSA was released from SMPs at a 50 mM glucose concentration after 72 h. A two-fold increase in transfection was observed in 50 mM glucose compared to that of 10 mM glucose.

Effect of Polymer Ionization on the Interaction with DNA in Nonviral Gene Delivery Systems

2003 ◽  
Vol 4 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Uracha Rungsardthong ◽  
Touraj Ehtezazi ◽  
Lindsey Bailey ◽  
Steven P. Armes ◽  
Martin C. Garnett ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

Photodegradable Neutral-Cationic Brush Block Copolymers for Nonviral Gene Delivery

2014 ◽  
Vol 9 (8) ◽  
pp. 2148-2155 ◽  
Author(s):  
Xianglong Hu ◽  
Yang Li ◽  
Tao Liu ◽  
Guoying Zhang ◽  
Shiyong Liu
Keyword(s):  
Block Copolymers ◽  
Gene Delivery ◽  
Nonviral Gene Delivery
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