scholarly journals Polyarginine Molecular Weight Determines Transfection Efficiency of Calcium Condensed Complexes

2013 ◽  
Vol 10 (5) ◽  
pp. 1940-1948 ◽  
Author(s):  
Nabil A. Alhakamy ◽  
Cory J. Berkland
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Condensed Complexes

Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

2019 ◽  
Vol 7 (4) ◽  
pp. 1716-1728 ◽  
Author(s):  
Huapan Fang ◽  
Lin Lin ◽  
Jie Chen ◽  
Jiayan Wu ◽  
Huayu Tian ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Zinc Ion ◽  
Low Molecular Weight ◽  
Gene Delivery System ◽  
System P

A zinc ion coordination-contained polycationic gene delivery system.

scholarly journals Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Yaguang Zhang ◽  
Hongmei Shu ◽  
Jing Hu ◽  
Min Zhang ◽  
Junweng Wu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Transfection Efficiency ◽  
Cross Linking ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Dna Complexes ◽  
Dynamic Source

A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake. The binding assessment of R13 toαvβ3 positive cells was performed by HRP labeling. The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency. The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency. The results indicated that the bifunctional peptide R13 had the ability of binding toαvβ3 positive cellsin vitro. The modification of P123-PEI-R13 with R13 made it display new property of internalization. P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route. After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.

Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

Acid-labile poly(amino alcohol ortho ester) based on low molecular weight polyethyleneimine for gene delivery

2017 ◽  
Vol 32 (3) ◽  
pp. 349-361 ◽  
Author(s):  
Jun Wang ◽  
Lei Zhang ◽  
Xin Wang ◽  
Shengxiang Fu ◽  
Guoqing Yan
Keyword(s):  
Molecular Weight ◽  
Amino Alcohol ◽  
Water Solubility ◽  
Transfection Efficiency ◽  
Enzymatic Digestion ◽  
Low Molecular Weight ◽  
Hydroxyl Groups ◽  
Ortho Ester ◽  
Human Neuroblastoma ◽  
Acid Labile

A series of acid-labile poly(amino alcohol ortho ester) (POEeis) were synthesized through ring-opening polymerization between diglycidyl ethers with ortho ester bonded and low molecular weight polyethyleneimine by various feed molar ratios. The obtain POEei 1 and POEei 2 exhibited clear kinetic of degradation and condensed plasmid DNA into nanoparticles of suitable sizes (250–300 nm) and positive zeta potentials (+20–30 mV) while protecting DNA from enzymatic digestion. Further, these polymers have uniform distribution of abundant hydroxyl groups, which could improve their water solubility, biocompatibility, and lower protein adsorption. Significantly, ortho ester groups in POEeis main-chains could hydrolyze rapidly at acidic endosomal pH, resulting in intracellular DNA release and diminished material toxicity. MTT assay revealed that all the polymers exhibited much lower cytotoxicity than 25 kDa PEI in the human neuroblastoma SH-SY5Y cells. Moreover, the transfection efficiency of POEei 1 was higher than 25 kDa PEI in serum-free medium or 10% serum medium.

Low molecular weight polyethyleneimine modified by 2-aminoimidazole achieving excellent gene transfection efficiency

2020 ◽  
Vol 140 ◽  
pp. 110017 ◽  
Author(s):  
Jie Song ◽  
Dongli Wang ◽  
Jing Wang ◽  
Qing Shen ◽  
Cao Xie ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Low Molecular Weight

scholarly journals A self-assembling polycationic nanocarrier that exhibits exceptional gene transfection efficiency

RSC Advances ◽  
2015 ◽  
Vol 5 (111) ◽  
pp. 91619-91632 ◽  
Author(s):  
Kishor Sarkar ◽  
Sai Rama Krishna Meka ◽  
Giridhar Madras ◽  
Kaushik Chatterjee
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Low Molecular Weight ◽  
High Transfection Efficiency ◽  
Self Assembling

A novel polycationic gene carrier was prepared by conjugation of low molecular weight polyethyleneimine with gelatin through 4-bromonaphthaleic anhydride with exceptionally high transfection efficiency.

Low molecular weight chitosan–protamine conjugate for siRNA delivery with enhanced stability and transfection efficiency

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110951-110963 ◽  
Author(s):  
Sushilkumar Patil ◽  
Priyanka Bhatt ◽  
Rohan Lalani ◽  
Jitendra Amrutiya ◽  
Imran Vhora ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Sirna Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan ◽  
Low Cytotoxicity ◽  
Enhanced Stability

Chitosan is among the few polymers with high biocompatibility and low cytotoxicity.

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