Nuclear Inclusion of Nontargeted and Chromatin-Targeted Polystyrene Beads and Plasmid DNA Containing Nanoparticles

Nathalie Symens; Rudolf Walczak; Joseph Demeester; Iain Mattaj; Stefaan C. De Smedt; Katrien Remaut

doi:10.1021/mp200120v

Nuclear Inclusion of Nontargeted and Chromatin-Targeted Polystyrene Beads and Plasmid DNA Containing Nanoparticles

Molecular Pharmaceutics ◽

10.1021/mp200120v ◽

2011 ◽

Vol 8 (5) ◽

pp. 1757-1766 ◽

Cited By ~ 20

Author(s):

Nathalie Symens ◽

Rudolf Walczak ◽

Joseph Demeester ◽

Iain Mattaj ◽

Stefaan C. De Smedt ◽

...

Keyword(s):

Plasmid Dna ◽

Nuclear Inclusion ◽

Polystyrene Beads

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Nuclear inclusion of inert and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles

Drug Discovery Today ◽

10.1016/j.drudis.2010.09.360 ◽

2010 ◽

Vol 15 (23-24) ◽

pp. 1082-1083

Author(s):

N. Symens ◽

R. Walzack ◽

J. Demeester ◽

I. Mattaj ◽

S. De Smedt ◽

...

Keyword(s):

Plasmid Dna ◽

Nuclear Inclusion ◽

Polystyrene Beads

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Nuclear inclusion of inert and chromatin-targeted polystyrene spheres and plasmid DNA containing nanoparticles

Journal of Controlled Release ◽

10.1016/j.jconrel.2010.07.071 ◽

2010 ◽

Vol 148 (1) ◽

pp. e96-e98 ◽

Cited By ~ 2

Author(s):

N. Symens ◽

R. Walzack ◽

J. Demeester ◽

I. Mattaj ◽

S.C. De Smedt ◽

...

Keyword(s):

Plasmid Dna ◽

Nuclear Inclusion ◽

Polystyrene Spheres

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Origin of Hepatic Nuclear Inclusion Bodies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072575 ◽

1973 ◽

Vol 31 ◽

pp. 426-427

Author(s):

F. G. Zaki ◽

J. A. Greenlee ◽

C. H. Keysser

Keyword(s):

Inclusion Bodies ◽

Storage Disease ◽

Glycogen Storage ◽

Nutritional Deficiencies ◽

Nuclear Inclusion ◽

Electron Microscopic ◽

Human Liver Cells ◽

Microscopic Studies ◽

Per Se ◽

Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

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Imaging sub-micron objects with light microscopy: Visualization of the T-4 bacteriophage

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156158 ◽

1989 ◽

Vol 47 ◽

pp. 834-835

Author(s):

Malcolm Brown ◽

Reynolds M. Delgado ◽

Michael J. Fink

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Focal Plane ◽

Phase Contrast ◽

Dark Field ◽

E Coli ◽

Polystyrene Beads ◽

Television Camera ◽

Transmission Electron ◽

Universal Microscope

While light microscopy has been used to image sub-micron objects, numerous problems with diffraction-limitations often preclude extraction of useful information. Using conventional dark-field and phase contrast light microscopy coupled with image processing, we have studied the following objects: (a) polystyrene beads (88nm, 264nm, and 557mn); (b) frustules of the diatom, Pleurosigma angulatum, and the T-4 bacteriophage attached to its host, E. coli or free in the medium. Equivalent images of the same areas of polystyrene beads and T-4 bacteriophages were produced using transmission electron microscopy.For light microscopy, we used a Zeiss universal microscope. For phase contrast observations a 100X Neofluar objective (N.A.=1.3) was applied. With dark-field, a 100X planachromat objective (N.A.=1.25) in combination with an ultra-condenser (N.A.=1.25) was employed. An intermediate magnifier (Optivar) was available to conveniently give magnification settings of 1.25, 1.6, and 2.0. The image was projected onto the back focal plane of a film or television camera with a Carl Zeiss Jena 18X Compens ocular.

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