Comparison of the Depot Effect and Immunogenicity of Liposomes Based on Dimethyldioctadecylammonium (DDA), 3β-[N-(N′,N′-Dimethylaminoethane)carbomyl] Cholesterol (DC-Chol), and 1,2-Dioleoyl-3-trimethylammonium Propane (DOTAP): Prolonged Liposome Retention Mediates Stronger Th1 Responses

2010 ◽  
Vol 8 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Malou Henriksen-Lacey ◽  
Dennis Christensen ◽  
Vincent W. Bramwell ◽  
Thomas Lindenstrøm ◽  
Else Marie Agger ◽  
...  
Keyword(s):  
Depot Effect

scholarly journals Dexamethasone-Loaded Lipomers: Development, Characterization, and Skin Biodistribution Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 533
Author(s):  
Eloy Pena-Rodríguez ◽  
Maria Lajarin-Reinares ◽  
Aida Mata-Ventosa ◽  
Sandra Pérez-Torras ◽  
Francisco Fernández-Campos
Keyword(s):  
In Vitro Release ◽  
Human Keratinocytes ◽  
Topical Administration ◽  
In Vitro Cytotoxicity ◽  
Hair Follicles ◽  
Vertical Diffusion ◽  
Biodistribution Studies ◽  
Follicular Targeting ◽  
Depot Effect

Follicular targeting has gained more attention in recent decades, due to the possibility of obtaining a depot effect in topical administration and its potential as a tool to treat hair follicle-related diseases. Lipid core ethyl cellulose lipomers were developed and optimized, following which characterization of their physicochemical properties was carried out. Dexamethasone was encapsulated in the lipomers (size, 115 nm; polydispersity, 0.24; zeta-potential (Z-potential), +30 mV) and their in vitro release profiles against dexamethasone in solution were investigated by vertical diffusion Franz cells. The skin biodistribution of the fluorescent-loaded lipomers was observed using confocal microscopy, demonstrating the accumulation of both lipomers and fluorochromes in the hair follicles of pig skin. To confirm this fact, immunofluorescence of the dexamethasone-loaded lipomers was carried out in pig hair follicles. The anti-inflammatory (via TNFα) efficacy of the dexamethasone-loaded lipomers was demonstrated in vitro in an HEK001 human keratinocytes cell culture and the in vitro cytotoxicity of the nanoformulation was investigated.

scholarly journals Depot effect of bioactive components in experimental membrane filtrations

2017 ◽  
Vol 780 ◽  
pp. 012005 ◽  
Author(s):  
D Mitev ◽  
D Peshev ◽  
G Peev ◽  
L Peeva
Keyword(s):  
Bioactive Components ◽  
Depot Effect

scholarly journals Impetiginized Dyshidrotic Eczema

2017 ◽  
Vol 5 (4) ◽  
pp. 539-540 ◽  
Author(s):  
Georgi Tchernev ◽  
Matteo Zanardelli ◽  
Cristiana Voicu ◽  
Ilko Bakardzhiev ◽  
Torello Lotti ◽  
...  
Keyword(s):  
Risk Factors ◽  
Clinical Case ◽  
Histological Analysis ◽  
Local Treatment ◽  
Extended Period ◽  
Corticosteroid Treatment ◽  
Clobetasol Propionate ◽  
Group I ◽  
Maximum Period ◽  
Depot Effect

A 16 years old female patient, affected by atopic dermatitis and rhinoconjunctivitis allergica since childhood, requested a dermatologic consultation for lesions which had appeared after 3 months of local treatment with clobethasole propionate. The histological analysis confirmed the diagnosis of dyshidrotic eczema and the microbiological smears demonstrated a significant infection with Staphylococcus aureus. The risk of developing corticosteroids’ side-effects depends on the potency of the product, extended period of use and the volume of product applied. Clobetasol propionate is a group I- highly potent corticosteroid, which should be used for a maximum period of 2 weeks. Several authors have found that this agent has cumulative depot effect, persisting in the epidermis for 4 days after only one application. Taking together these observations, sustained by the clinical case presented above, we can conclude that the infectious risks associated with topical corticosteroid treatment must not be neglected, particularly since treated patients are fragile, and frequently have multiple well-known risk factors.

Pegylation of DDA:TDB liposomal adjuvants reduces the vaccine depot effect and alters the Th1/Th2 immune responses

2012 ◽  
Vol 158 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Randip Kaur ◽  
Vincent W. Bramwell ◽  
Daniel J. Kirby ◽  
Yvonne Perrie
Keyword(s):  
Immune Responses ◽  
Depot Effect

Dual dye in-vivo imaging of differentially charged PLGA carriers reveals antigen-depot effect, leading to improved immune responses in preclinical models

2018 ◽  
Vol 117 ◽  
pp. 88-97 ◽  
Author(s):  
Markus Riehl ◽  
Meike Harms ◽  
Henrike Lucas ◽  
Thomas Ebensen ◽  
Carlos A. Guzmán ◽  
...  
Keyword(s):  
Immune Responses ◽  
In Vivo Imaging ◽  
Preclinical Models ◽  
Depot Effect
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