High-Resolution Topographic Imaging of Environmentally Responsive, Elastin-Mimetic Hydrogels

1999 ◽  
Vol 32 (26) ◽  
pp. 9067-9070 ◽  
Author(s):  
R. Andrew McMillan ◽  
Kevin L. Caran ◽  
Robert P. Apkarian ◽  
Vincent P. Conticello
Keyword(s):  
High Resolution ◽  
Environmentally Responsive ◽  
Topographic Imaging

Hopping Probe Ion Conductance Microscopy and its Combined Patch-Clamping：A Powerful Tool for Structural and Functional Studies in Neural Nanobiology

2011 ◽  
Vol 694 ◽  
pp. 54-58
Author(s):  
Xin Liang Zhao ◽  
Xiao Liu ◽  
Hu Jie Lu ◽  
Li Ying Ma ◽  
Rui Ling Gao ◽  
...  
Keyword(s):  
High Resolution ◽  
Patch Clamp ◽  
Ion Channel ◽  
Membrane Structures ◽  
Patch Clamp Technique ◽  
Ion Conductance ◽  
Resolution Imaging ◽  
Living Neurons ◽  
Topographic Imaging ◽  
The Relationship

Continuous high-resolution observations of cell membrane would greatly aid the elucidation of the relationship between structure and function and facilitate the study of physiological processing in cell biology. However, high-resolution studying living neuron membrane structures and its functions is still a challenge in current nanobiology. The new developed Hoping Probe Ion Conductance Microscopy (HPICM) is designed for non-contact continuous high-resolution topographic imaging of living cells under physiological conditions. In this review, we concisely introduced the basic operation principle of HPICM and its applications in high spatial resolution imaging of two living neuron cell models, N-type SK-N-SH cells and NGF-differentiated sympathetic neuron-like PC12 cells. Combining HPICM with patch-clamp technique, we further investigated the functional ion-channel of under-differentiated neuron-like PC12 cells and demonstrated that NGF treatment promoted the outgrowth of neurites and increased the activity of TTX-sensitive sodium channel. All these results demonstrate that HPICM combined with patch-clamp technique offers high-resolution topographic imaging of living neurons with non-contact — making HPICM an ideal high-resolution imaging technique not to interact/interfere with living neurons during image acquisition, and provides detailed information about the relationship between membrane structures and ion-channel functions of living neurons at the same time, which has the potential to become a powerful microscopy for in-depth researching in neural nanobiology.

Characterizing the Hemming Performance of Automotive Aluminum Alloys With High-Resolution Topographic Imaging

2014 ◽  
Vol 136 (3) ◽  
Author(s):  
M. R. Stoudt ◽  
J. B. Hubbard ◽  
J. E. Carsley ◽  
S. E. Hartfield-Wünsch
Keyword(s):  
Mechanical Properties ◽  
Aluminum Alloys ◽  
High Resolution ◽  
Surface Morphology ◽  
Surface Cracks ◽  
Surface Displacements ◽  
Quantitative Surface Analysis ◽  
Quantitative Analyses ◽  
Surface Morphologies ◽  
Topographic Imaging

We used high-resolution quantitative surface analysis to evaluate the surfaces of two aluminum automotive closure panel alloys, which were bent to a 180 deg angle in a simulated hemming test. Maps of the displacements normal to the sheet were superimposed on the topographies to correlate the location of the maximum displacements and the surface morphology. While the alloys had similar mechanical properties, quantitative analyses yielded considerable differences in the deformed surface morphologies. One alloy had a greater density and broader size distribution of constituent particles, which increased the likelihood for particle decohesion. This resulted in large surface displacements that were uncorrelated with the underlying microstructure. While no splitting was observed in either alloy, large uncorrelated surface displacements could indicate the presence of short surface cracks.

A field-emission SEM optimized for operation at low beam voltage

1993 ◽  
Vol 51 ◽  
pp. 630-631
Author(s):  
JB Pawley ◽  
J Ximen ◽  
PS-D Lin ◽  
M. Schippert
Keyword(s):  
High Resolution ◽  
Radiation Damage ◽  
Field Emission ◽  
Secondary Electron ◽  
Structural Features ◽  
Objective Lens ◽  
Beam Diameter ◽  
Beam Voltage ◽  
High Resolution Images ◽  
Topographic Imaging

The advantages of operating the SEM at low beam voltage (V0 ) have been recognized for some time. They include: less specimen charging, greater contrast in the fine topographic component of the secondary electron (SE) signal and reduced radiation damage. Although initially it was difficult to obtain high resolution images when using low V0, this limitation can be essentially overcome by employing both a FE source and an immersion objective lens. In an instrument employing both of these features it is possible to produce a beam diameter of about 3 nm @ 1.5 kV. When insulating specimens are viewed under these conditions, the resolution in the image is limited more by the structure of the coating material than by the beam diameter, while on conductors, small structural features produce useful contrast only at low V0.The remaining obstacle to more widespread use of LVSEM for high resolution topographic imaging is the high cost of the equipment.

Observations of the spatial structure of interstellar hydrogen

1967 ◽  
Vol 31 ◽  
pp. 45-46
Author(s):  
Carl Heiles
Keyword(s):  
High Resolution ◽  
Spatial Structure ◽  
Velocity Dispersion ◽  
Temperature Variations

High-resolution 21-cm line observations in a region aroundlII= 120°,b11= +15°, have revealed four types of structure in the interstellar hydrogen: a smooth background, large sheets of density 2 atoms cm-3, clouds occurring mostly in groups, and ‘Cloudlets’ of a few solar masses and a few parsecs in size; the velocity dispersion in the Cloudlets is only 1 km/sec. Strong temperature variations in the gas are in evidence.

Combining integrated systems-biology approaches with intervention-based experimental design provides a higher-resolution path forward for microbiome research

2019 ◽  
Vol 42 ◽  
Author(s):  
J. Alfredo Blakeley-Ruiz ◽  
Carlee S. McClintock ◽  
Ralph Lydic ◽  
Helen A. Baghdoyan ◽  
James J. Choo ◽  
...  
Keyword(s):  
Systems Biology ◽  
High Resolution ◽  
Experimental Design ◽  
Integrated Systems ◽  
Microbiome Research ◽  
Constructive Criticism

Abstract The Hooks et al. review of microbiota-gut-brain (MGB) literature provides a constructive criticism of the general approaches encompassing MGB research. This commentary extends their review by: (a) highlighting capabilities of advanced systems-biology “-omics” techniques for microbiome research and (b) recommending that combining these high-resolution techniques with intervention-based experimental design may be the path forward for future MGB research.

Very High Resolution Analysis of the Dynamics of a Coronal Plasmoid

1994 ◽  
Vol 144 ◽  
pp. 593-596
Author(s):  
O. Bouchard ◽  
S. Koutchmy ◽  
L. November ◽  
J.-C. Vial ◽  
J. B. Zirker
Keyword(s):  
High Resolution ◽  
Solar Eclipse ◽  
Total Solar Eclipse ◽  
Field Of View ◽  
Small Field ◽  
High Resolution Analysis ◽  
Ccd Observations ◽  
Resolution Analysis ◽  
Very High ◽  
Small Field Of View

AbstractWe present the results of the analysis of a movie taken over a small field of view in the intermediate corona at a spatial resolution of 0.5“, a temporal resolution of 1 s and a spectral passband of 7 nm. These CCD observations were made at the prime focus of the 3.6 m aperture CFHT telescope during the 1991 total solar eclipse.

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