Molecular Weights of Homogeneous Samples of Deoxyribonucleic Acid Determined from Hydrodynamic Theories for the Wormlike Coil

1968 ◽  
Vol 1 (6) ◽  
pp. 491-494 ◽  
Author(s):  
John E. Hearst ◽  
Carl W. Schmid ◽  
Frank P. Rinehart
Keyword(s):  
Deoxyribonucleic Acid ◽  
Molecular Weights

scholarly journals Ultrasonic Scission of Deoxyribonucleic Acid in Aqueous Solution I. Conditions for Sonication and Molecular Weights of Sonicated Samples

1986 ◽  
Vol 18 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Kiyohiro Fukudome ◽  
Kiwamu Yamaoka ◽  
Kenichiro Nishikori ◽  
Takamichi Takahashi ◽  
Osamu Yamamoto
Keyword(s):  
Aqueous Solution ◽  
Deoxyribonucleic Acid ◽  
Molecular Weights

scholarly journals Molecular Weights and Physical Properties of Deoxyribonucleic Acid

Nature ◽  
1957 ◽  
Vol 180 (4598) ◽  
pp. 1340-1340 ◽  
Author(s):  
J. A. V. BUTLER ◽  
D. J. R. LAURENCE ◽  
A. B. ROBINS ◽  
K. V. SHOOTER
Keyword(s):  
Physical Properties ◽  
Deoxyribonucleic Acid ◽  
Molecular Weights

Phleomycin-stimulated solubilization of deoxyribonucleic acid in Escherichia coli. II. Inhibition of solubilization by bacteriophage T4

1976 ◽  
Vol 22 (5) ◽  
pp. 645-653 ◽  
Author(s):  
Larry D. Farrell ◽  
Harvard Reiter
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Deoxyribonucleic Acid ◽  
Cytoplasmic Membrane ◽  
Bacteriophage T4 ◽  
Continuous Exposure ◽  
Dna Endonuclease ◽  
Molecular Weights ◽  
Dependent Inhibition ◽  
Antibiotic Inhibition

Phleomycin-stimulated solubilization of Escherichia coli DNA is inhibited by infecting the cells with mutants of bacteriophage T4 before treatment with the antibiotic, inhibition requiring phage-specified protein synthesis. Two different modes of inhibition can be differentiated by infecting with mutants which are defective in an early state (gene den A−; endonuclease II-independent inhibition) or a late stage (gene 46−; endonuclease II-dependent inhibition) of phage-directed degradation of host DNA. Endonuclease II-independent inhibition results from interference with phleomycin-induced release of host DNA from the cytoplasmic membrane. In the presence of endonuclease II, the host DNA is converted to fragments, with average molecular weights of 106 daltons, the further degradation of which is not promoted by continuous exposure of the cells to phleomycin.

Analysis of the thermal transition curves of deoxyribonucleic acid from microorganisms

1970 ◽  
Vol 16 (10) ◽  
pp. 989-995 ◽  
Author(s):  
R. E. Krieg ◽  
W. R. Lockhart
Keyword(s):  
Base Composition ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Size Range ◽  
Thermal Transition ◽  
Molecular Fragments ◽  
Frequency Distributions ◽  
Molecular Weights ◽  
Intact Molecule ◽  
Transition Curves

The thermal transition of sheared deoxyribonucleic acid (DNA) may reveal differing nucleotide frequencies within individual fragments, which is a crude reflection of nucleotide sequence in the intact molecule. DNA samples from seven species were characterized at three molecular weights, encompassing a size range from about one one-hundredth to one ten-thousandth of the original molecules. Thermal denaturation curves were treated as cumulative frequency distributions of individual molecular fragments differing in average base composition. Thermal transition curves of individual DNA samples show characteristic deviations from normality, which indicates that the proportions of nucleotides in some DNA fragments differ considerably from the average for the intact molecule. The statistical values describing denaturation curves are characteristic and constant for DNA from a given organism and are different and distinctive for different organisms.

scholarly journals Studies on the accessibility of deoxyribonucleic acid in deoxyribonucleoprotein to cationic molecules

1971 ◽  
Vol 122 (4) ◽  
pp. 583-592 ◽  
Author(s):  
Ruth F. Itzhaki
Keyword(s):  
Molecular Weight ◽  
Toluidine Blue ◽  
Deoxyribonucleic Acid ◽  
Cetylpyridinium Chloride ◽  
Gene Repression ◽  
Cross Linking ◽  
Large Molecules ◽  
Molecular Weights ◽  
Complete Precipitation ◽  
Phosphate Groups

The binding of deoxyribonucleoprotein to Toluidine Blue, to cetylpyridinium chloride and to polylysine of various molecular weights was studied to determine the percentage of free DNA phosphate groups in deoxyribonucleoprotein. Binding was measured by addition of these reagents to deoxyribonucleoprotein at a range of concentrations such that complete precipitation of the deoxyribonucleoprotein occurred. With Toluidine Blue the binding corresponded to about 48% of the DNA phosphates in deoxyribonucleoprotein. The dye did not cause appreciable displacement of protein from the DNA. With cetylpyridinium chloride the binding corresponded to about 41% of the DNA phosphates. With polylysine preparations of molecular weight 1250 and 7790 the binding values for deoxyribonucleoprotein were 46 and 38% respectively. The results suggest that the free phosphates lie in stretches sufficiently long to accommodate most of each polylysine molecule. With polylysine of molecular weight 62000 cross-linking of free stretches of DNA on different deoxyribonucleoprotein molecules probably occurs. It is concluded that although most of the free phosphates are probably ‘hidden’ beneath covering histone, corresponding perhaps to runs of non-basic residues in the latter, they are surprisingly accessible to very large molecules. The relevance of this finding to the problem of gene repression is discussed.

scholarly journals Ultrasonic Scission of Deoxyribonucleic Acid in Aqueous Solution II. Precipitational Fractionation and Molecular Weights of Sonicated Samples

1986 ◽  
Vol 18 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Kiyohiro Fukudome ◽  
Kiwamu Yamaoka ◽  
Kenichiro Nishikori ◽  
Hidekazu Tatehata ◽  
Osamu Yamamoto
Keyword(s):  
Aqueous Solution ◽  
Deoxyribonucleic Acid ◽  
Molecular Weights
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