Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures

2014 ◽  
Vol 47 (11) ◽  
pp. 3582-3586 ◽  
Author(s):  
Xiang Li ◽  
Kateryna Khairulina ◽  
Ung-il Chung ◽  
Takamasa Sakai
Keyword(s):  
Electrophoretic Mobility ◽  
Polymer Solutions ◽  
Double Stranded Dna

Electrophoretic mobility of linear and star-branched DNA in semidilute polymer solutions

2006 ◽  
Vol 27 (16) ◽  
pp. 3181-3194 ◽  
Author(s):  
Sourav Saha ◽  
Daniel M. Heuer ◽  
Lynden A. Archer
Keyword(s):  
Electrophoretic Mobility ◽  
Polymer Solutions ◽  
Branched Dna

Electrophoretic mobility of semi-flexible double-stranded DNA in defect-controlled polymer networks: Mechanism investigation and role of structural parameters

2015 ◽  
Vol 142 (23) ◽  
pp. 234904 ◽  
Author(s):  
Kateryna Khairulina ◽  
Xiang Li ◽  
Kengo Nishi ◽  
Mitsuhiro Shibayama ◽  
Ung-il Chung ◽  
...  
Keyword(s):  
Electrophoretic Mobility ◽  
Structural Parameters ◽  
Polymer Networks ◽  
Double Stranded Dna

Investigation of migration behavior of rod-like dsDNA in gel with precisely controlled network structure

2014 ◽  
Vol 1622 ◽  
pp. 169-174
Author(s):  
Xiang Li ◽  
Kateryna Khairulina ◽  
Ung-il Chung ◽  
Takamasa Sakai
Keyword(s):  
Network Structure ◽  
High Performance ◽  
Polymer Solutions ◽  
Volume Fraction ◽  
Polymer Network ◽  
Migration Behavior ◽  
Size Separation ◽  
Double Stranded Dna ◽  
Homogeneous Network ◽  
Polymer Volume

ABSTRACTWe investigated the migration behavior of rodlike double-stranded DNA (dsDNA) in polymer gels and polymer solutions. Tetra-PEG gel, which has a homogeneous network structure, was utilized as a model system, allowing us to systematically tune the polymer volume fraction and molecular weight of network strand. Although we examined the applicability of the existing models, all the models failed to predict the migration behavior. Thus, we proposed a new model based on the Ogston model, which well explained the experimental data of polymer solutions and gels. The polymer volume fraction determined the maximum mobility, while the network strand governed the size sieving effect. From these results, we conclude that the polymer network with lower polymer volume fraction and smaller network strand is better in terms of size separation. The homogeneous polymer network is vital for understanding of particles’ dynamics in polymer network and a promising material for high-performance size separation.

Zinc-Finger-Protein-Based Microfluidic Electrophoretic Mobility Reversal Assay for Quantitative Double-Stranded DNA Analysis

2021 ◽  
Author(s):  
Nebiyu Getachew Arega ◽  
Whitney N. Heard ◽  
Nguyen Anh Nhung Tran ◽  
Sukyo Jung ◽  
Jianyun Meng ◽  
...  
Keyword(s):  
Electrophoretic Mobility ◽  
Zinc Finger ◽  
Dna Analysis ◽  
Zinc Finger Protein ◽  
Double Stranded Dna ◽  
Finger Protein ◽  
Mobility Reversal
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