Effect of matrix chain length on the electrophoretic mobility of large linear and branched DNA in polymer solutions

2004 ◽  
Vol 25 (3) ◽  
pp. 396-404 ◽  
Author(s):  
Sourav Saha ◽  
Daniel M. Heuer ◽  
Lynden A. Archer
Keyword(s):  
Electrophoretic Mobility ◽  
Chain Length ◽  
Polymer Solutions ◽  
Branched Dna

Electrophoretic mobility of linear and star-branched DNA in semidilute polymer solutions

2006 ◽  
Vol 27 (16) ◽  
pp. 3181-3194 ◽  
Author(s):  
Sourav Saha ◽  
Daniel M. Heuer ◽  
Lynden A. Archer
Keyword(s):  
Electrophoretic Mobility ◽  
Polymer Solutions ◽  
Branched Dna

Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures

2014 ◽  
Vol 47 (11) ◽  
pp. 3582-3586 ◽  
Author(s):  
Xiang Li ◽  
Kateryna Khairulina ◽  
Ung-il Chung ◽  
Takamasa Sakai
Keyword(s):  
Electrophoretic Mobility ◽  
Polymer Solutions ◽  
Double Stranded Dna

Chain length dependence of liquid—liquid equilibria of binary polymer solutions

Polymer ◽  
1998 ◽  
Vol 39 (8-9) ◽  
pp. 1735-1739 ◽  
Author(s):  
Bong Ho Chang ◽  
Kyong-Ok Ryu ◽  
Young Chan Bae
Keyword(s):  
Chain Length ◽  
Polymer Solutions ◽  
Length Dependence ◽  
Binary Polymer

scholarly journals Diffusive properties of solvent molecules in the neighborhood of a polymer chain as seen by Monte-Carlo simulations

Soft Matter ◽  
2016 ◽  
Vol 12 (25) ◽  
pp. 5519-5528 ◽  
Author(s):  
M. Kozanecki ◽  
K. Halagan ◽  
J. Saramak ◽  
K. Matyjaszewski
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Simulations ◽  
Chain Length ◽  
Polymer Chain ◽  
Polymer Solutions ◽  
Solvent Molecules

The influence of both polymer chain length and concentration on the mobility of solvent molecules in polymer solutions was studied by Monte Carlo simulations with the use of the dynamic lattice liquid (DLL) model.

Analysis of branched nucleic acid structure using comparative gel electrophoresis

2008 ◽  
Vol 41 (1) ◽  
pp. 1-39 ◽  
Author(s):  
David M. J. Lilley
Keyword(s):  
Nucleic Acids ◽  
Electrophoretic Mobility ◽  
Gel Electrophoresis ◽  
Holliday Junction ◽  
Dna And Rna ◽  
Dna Junctions ◽  
Branched Dna ◽  
Powerful Means ◽  
Crystallographic Structures ◽  
Acid Structure

AbstractElectrophoresis in polyacrylamide gels provides a simple yet powerful means of analyzing the relative disposition of helical arms in branched nucleic acids. The electrophoretic mobility of DNA or RNA with a central discontinuity is determined by the angle subtended between the arms radiating from the branchpoint. In a multi-helical branchpoint, comparative gel electrophoresis can provide a relative measure of all the inter-helical angles and thus the shape and symmetry of the molecule. Using the long–short arm approach, the electrophoretic mobility of all the species with two helical arms that are longer than all others is compared. This can be done as a function of conditions, allowing the analysis of ion-dependent folding of branched DNA and RNA species. Notable successes for the technique include the four-way (Holliday) junction in DNA and helical junctions in functionally significant RNA species such as ribozymes. Many of these structures have subsequently been proved correct by crystallography or other methods, up to 10 years later in the case of the Holliday junction. Just as important, the technique has not failed to date. Comparative gel electrophoresis can provide a window on both fast and slow conformational equilibria such as conformer exchange in four-way DNA junctions. But perhaps the biggest test of the approach has been to deduce the structures of complexes of four-way DNA junctions with proteins. Two recent crystallographic structures show that the global structures were correctly deduced by electrophoresis, proving the worth of the method even in these rather complex systems. Comparative gel electrophoresis is a robust method for the analysis of branched nucleic acids and their complexes.

Can the Miscibility of Telechelic Polymer Solutions Increase with Polymer Chain Length?

2011 ◽  
Vol 1 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Jacek Dudowicz ◽  
Karl F. Freed ◽  
Jack F. Douglas
Keyword(s):  
Chain Length ◽  
Polymer Chain ◽  
Polymer Solutions ◽  
Telechelic Polymer

Termination in Semi-Dilute and Concentrated Polymer Solutions

2009 ◽  
Vol 62 (8) ◽  
pp. 857 ◽  
Author(s):  
Geoffrey Johnston-Hall ◽  
Michael J. Monteiro
Keyword(s):  
Chain Length ◽  
Power Law ◽  
Polymer Solutions ◽  
Polymer Concentration ◽  
Star Polymer ◽  
Polymer Systems ◽  
Theoretical Predictions ◽  
The Matrix ◽  
Concentrated Solution ◽  
Blob Model

The aim of the present work was to develop a deeper understanding into termination processes in the semi-dilute and concentrated regimes. The study was carried out to examine the effect of termination between linear polystyrene radical chains in linear, four-arm star, and six-arm star polymer systems using the reversible addition–fragmentation chain transfer chain length-dependent termination method. In particular, the power-law dependencies of both chain length and polymer concentration were evaluated in the semi-dilute and concentrated regimes. We found that theoretical predictions based on the blob model were in good agreement with the experimentally observed evolution of the rate coefficient for biomolecular termination, kti,i(x), in the semi-dilute solution regime. In addition, solvent quality was found to decrease with increasing chain length, increasing polymer concentration and as a function of the matrix topology (i.e. for star polymer solutions). In the concentrated solution regime, the role of chain entanglements became evident by determining the conversion-dependent power-law exponent, βgel (where kt ≈ x–βgel), which increased in the order: linear < four-arm star < six-arm star polymer systems. Above the critical chain length ic, termination was found to be primarily conversion-dependent, implying entanglements dominated termination between linear polymeric radicals. Although this may suggest that reptation plays an important role, our data are in disagreement with this theory, suggesting that the polymer matrix cannot be regarded as static or immobile on the diffusion time scales for bimolecular termination.

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