Anomalous Conformational Instability and Hydrogel Formation of a Cationic Class of Self-Assembling Oligopeptides

Thomas J. Measey; Reinhard Schweitzer-Stenner; Vijoya Sa; Konstantin Kornev

doi:10.1021/ma101450b

Supramolecular Hydrogel Formation in a Series of Self-Assembling Lipopeptides with Varying Lipid Chain Length

Biomacromolecules ◽

10.1021/acs.biomac.7b00057 ◽

2017 ◽

Vol 18 (7) ◽

pp. 2013-2023 ◽

Cited By ~ 15

Author(s):

V. Castelletto ◽

A. Kaur ◽

R. M. Kowalczyk ◽

I. W. Hamley ◽

M. Reza ◽

...

Keyword(s):

Chain Length ◽

Supramolecular Hydrogel ◽

Self Assembling ◽

Hydrogel Formation ◽

Lipid Chain

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Monitoring the Early Stage Self-Assembly of Enzyme-Assisted Peptide Hydrogels

Australian Journal of Chemistry ◽

10.1071/ch12557 ◽

2013 ◽

Vol 66 (5) ◽

pp. 572 ◽

Cited By ~ 9

Author(s):

Richard J. Williams ◽

James Gardiner ◽

Anders B. Sorensen ◽

Silvia Marchesan ◽

Roger J. Mulder ◽

...

Keyword(s):

1H Nmr ◽

Self Assembly ◽

Early Stage ◽

The Self ◽

Spectroscopic Techniques ◽

Subsequent Formation ◽

Self Assembling ◽

Hydrogel Formation ◽

Assembly Kinetics ◽

Peptide Hydrogels

The early stages of the self-assembly of peptide hydrogels largely determine their final material properties. Here we discuss experimental methodologies for monitoring the self-assembly kinetics which underpin peptide hydrogel formation. The early stage assembly of an enzyme-catalysed Fmoc-trileucine based self-assembled hydrogel was examined using spectroscopic techniques (circular dichroism, CD, and solution NMR) as well as chromatographic (HPLC) and mechanical (rheology) techniques. Optimal conditions for enzyme-assisted hydrogel formation were identified and the kinetics examined. A lag time associated with the formation and accumulation of the self-assembling peptide monomer was observed and a minimum hydrogelator concentration required for gelation was identified. Subsequent formation of well defined nano- and microscale structures lead to self-supporting hydrogels at a range of substrate and enzyme concentrations. 1H NMR monitoring of the early self-assembly process revealed trends that were well in agreement with those identified using traditional methods (i.e. HPLC, CD, rheology) demonstrating 1H NMR spectroscopy can be used to non-invasively monitor the self-assembly of peptide hydrogels without damaging or perturbing the system.

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Co-assembly and multicomponent hydrogel formation upon mixing nucleobase-containing peptides

Nanoscale ◽

10.1039/d1nr02417e ◽

2021 ◽

Author(s):

Tristan Giraud ◽

Sabine Bouguet-Bonnet ◽

Marie-José Stébé ◽

Lionel Richaudeau ◽

Guillaume Pickaert ◽

...

Keyword(s):

Soft Material ◽

Self Assembling ◽

Hydrogel Formation ◽

Ordered Nanostructures

Peptide-based hydrogels are physical gels formed through specific supramolecular self-assembling processes, leading to ordered nanostructures which constitutes the water entrapping scaffold of the soft material. Thanks to the inherent properties...

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Development of an Amino Sugar-Based Supramolecular Hydrogelator with Reduction Responsiveness

10.26434/chemrxiv.14152316.v1 ◽

2021 ◽

Author(s):

Sayuri L. Higashi ◽

Masato Ikeda

Keyword(s):

Molecular Weight ◽

Molecular Design ◽

Soft Materials ◽

Amino Sugar ◽

Low Molecular Weight ◽

Stimuli Responsive ◽

Supramolecular Hydrogel ◽

Self Assembling ◽

Hydrogel Formation

Stimuli-responsive supramolecular hydrogels are a newly emerging class of aqueous soft materials with a wide variety of bioapplications. Here we report a reduction-responsive supramolecular hydrogel constructed from a markedly simple low-molecular-weight hydrogelator, which is developed on the basis of modular molecular design containing a hydrophilic amino sugar and a reduction-responsive nitrophenyl group. The hydrogel formation ability differs significantly between glucosamine- and galactosamine-based self-assembling molecules, which are epimers at the C4 position, and only the glucosamine-based derivative can act as a hydrogelator.

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Compartmentalized Multilayer Hydrogel Formation Using a Stimulus-Responsive Self-Assembling Polysaccharide

ACS Applied Materials & Interfaces ◽

10.1021/am405544r ◽

2014 ◽

Vol 6 (4) ◽

pp. 2948-2957 ◽

Cited By ~ 27

Author(s):

Yuan Xiong ◽

Kun Yan ◽

William E. Bentley ◽

Hongbing Deng ◽

Yumin Du ◽

...

Keyword(s):

Self Assembling ◽

Stimulus Responsive ◽

Hydrogel Formation

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Development of an Amino Sugar-Based Supramolecular Hydrogelator with Reduction Responsiveness

10.26434/chemrxiv.14152316 ◽

2021 ◽

Author(s):

Sayuri L. Higashi ◽

Masato Ikeda

Keyword(s):

Molecular Weight ◽

Molecular Design ◽

Soft Materials ◽

Amino Sugar ◽

Low Molecular Weight ◽

Stimuli Responsive ◽

Supramolecular Hydrogel ◽

Self Assembling ◽

Hydrogel Formation

Stimuli-responsive supramolecular hydrogels are a newly emerging class of aqueous soft materials with a wide variety of bioapplications. Here we report a reduction-responsive supramolecular hydrogel constructed from a markedly simple low-molecular-weight hydrogelator, which is developed on the basis of modular molecular design containing a hydrophilic amino sugar and a reduction-responsive nitrophenyl group. The hydrogel formation ability differs significantly between glucosamine- and galactosamine-based self-assembling molecules, which are epimers at the C4 position, and only the glucosamine-based derivative can act as a hydrogelator.

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Rational design of a photo-responsive UVR8-derived protein and a self-assembling peptide–protein conjugate for responsive hydrogel formation

Nanoscale ◽

10.1039/c5nr05213k ◽

2015 ◽

Vol 7 (40) ◽

pp. 16666-16670 ◽

Cited By ~ 30

Author(s):

Xiaoli Zhang ◽

Chunming Dong ◽

Weiyun Huang ◽

Huaimin Wang ◽

Ling Wang ◽

...

Keyword(s):

Rational Design ◽

Self Assembling ◽

Protein Conjugate ◽

Hydrogel Formation

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Torus Circumference and Thickness Parameters From a Linear DNA Size Series Suggest How Toruses Self-Assemble

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119430 ◽

1985 ◽

Vol 43 ◽

pp. 522-523

Author(s):

George C. Ruben ◽

Kenneth A. Marx

Keyword(s):

Tertiary Structure ◽

Dna Complexes ◽

Tertiary Structures ◽

Self Assembling ◽

Double Stranded Dna ◽

Calf Thymus ◽

Dna Organization ◽

Condensed Dna ◽

Dna Size

Certain double stranded DNA bacteriophage and viruses are thought to have their DNA organized into large torus shaped structures. Morphologically, these poorly understood biological DNA tertiary structures resemble spermidine-condensed DNA complexes formed in vitro in the total absence of other macromolecules normally synthesized by the pathogens for the purpose of their own DNA packaging. Therefore, we have studied the tertiary structure of these self-assembling torus shaped spermidine- DNA complexes in a series of reports. Using freeze-etch, low Pt-C metal (10-15Å) replicas, we have visualized the microscopic DNA organization of both calf Thymus( CT) and linear 0X-174 RFII DNA toruses. In these structures DNA is circumferentially wound, continuously, around the torus into a semi-crystalline, hexagonal packed array of parallel DNA helix sections.

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