Functional Layer-By-Layer Design of Xerogel-Based First-Generation Amperometric Glucose Biosensors

Langmuir ◽  
2015 ◽  
Vol 31 (4) ◽  
pp. 1547-1555 ◽  
Author(s):  
Nicholas G. Poulos ◽  
Jackson R. Hall ◽  
Michael C. Leopold
Keyword(s):  
First Generation ◽  
Layer By Layer ◽  
Functional Layer ◽  
Glucose Biosensors ◽  
Amperometric Glucose Biosensors

Amperometric Glucose Biosensors Based on Composite Polymeric Structures to Prevent Interferences

2000 ◽  
Vol 33 (9) ◽  
pp. 1733-1753 ◽  
Author(s):  
Pascal Mailley ◽  
Serge Cosnier ◽  
Liliance Coche-Guérente
Keyword(s):  
Glucose Biosensors ◽  
Amperometric Glucose Biosensors ◽  
Polymeric Structures

Facile hydrothermal synthesis of mn doped ZnO nanopencils for development of amperometric glucose biosensors

2018 ◽  
Vol 5 (5) ◽  
pp. 055031 ◽  
Author(s):  
Mayoorika Shukla ◽  
Pramila ◽  
Jitesh Agrawal ◽  
Tejendra Dixit ◽  
I A Palani ◽  
...  
Keyword(s):  
Hydrothermal Synthesis ◽  
Glucose Biosensors ◽  
Doped Zno ◽  
Amperometric Glucose Biosensors ◽  
Mn Doped

Functional Layer-by-Layer Thin Films of Inducible Nitric Oxide (NO) Synthase Oxygenase and Polyethylenimine: Modulation of Enzyme Loading and NO-Release Activity

2018 ◽  
Vol 10 (9) ◽  
pp. 7745-7755 ◽  
Author(s):  
Bhagya Gunasekera ◽  
Charbel Abou Diwan ◽  
Ghaith Altawallbeh ◽  
Haitham Kalil ◽  
Shaimaa Maher ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Thin Films ◽  
No Synthase ◽  
Layer By Layer ◽  
Enzyme Loading ◽  
Functional Layer ◽  
No Release ◽  
Inducible Nitric Oxide

scholarly journals First Generation Amperometric Biosensing of Galactose with Xerogel-Carbon Nanotube Layer-By-Layer Assemblies

Nanomaterials ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 42 ◽  
Author(s):  
Najwa Labban ◽  
Mulugeta Wayu ◽  
Ciara Steele ◽  
Tess Munoz ◽  
Julie Pollock ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
First Generation ◽  
Fast Response ◽  
Enzyme Concentration ◽  
Layer By Layer ◽  
Serum Samples ◽  
Anionic Polymer ◽  
Drying Time ◽  
Silane Precursor ◽  
Walled Carbon Nanotubes

A first-generation amperometric galactose biosensor has been systematically developed utilizing layer-by-layer (LbL) construction of xerogels, polymers, and carbon nanotubes toward a greater fundamental understanding of sensor design with these materials and the potential development of a more efficient galactosemia diagnostic tool for clinical application. The effect of several parameters (xerogel silane precursor, buffer pH, enzyme concentration, drying time and the inclusion of a polyurethane (PU) outer layer) on galactose sensitivity were investigated with the critical nature of xerogel selection being demonstrated. Xerogels formed from silanes with medium, aliphatic side chains were shown to exhibit significant enhancements in sensitivity with the addition of PU due to decreased enzyme leaching. Semi-permeable membranes of diaminobenzene and resorcinol copolymer and Nafion were used for selective discrimination against interferent species and the accompanying loss of sensitivity with adding layers was countered using functionalized, single-walled carbon nanotubes (CNTs). Optimized sensor performance included effective galactose sensitivity (0.037 μA/mM) across a useful diagnostic concentration range (0.5 mM to 7 mM), fast response time (~30 s), and low limits of detection (~80 μM) comparable to literature reports on galactose sensors. Additional modification with anionic polymer layers and/or nanoparticles allowed for galactose detection in blood serum samples and additional selectivity effectiveness.

scholarly journals Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 50 ◽  
Author(s):  
Hao-Chun Chiang ◽  
Yanyan Wang ◽  
Qi Zhang ◽  
Kalle Levon
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Aqueous Electrolyte ◽  
Label Free ◽  
Reduction Step ◽  
Glucose Biosensors ◽  
Highly Sensitive ◽  
Amperometric Glucose Biosensors ◽  
Aunp Surface

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (> 50 μM) and a wide linear range after optimization with AuNP electrodeposition.

Towards direct enzyme wiring: a theoretical investigation of charge carrier transfer mechanisms between glucose oxidase and organic semiconductors

2019 ◽  
Vol 21 (6) ◽  
pp. 2968-2976 ◽  
Author(s):  
Gintautas Bagdžiūnas ◽  
Arūnas Ramanavičius
Keyword(s):  
Charge Transfer ◽  
Charge Carrier ◽  
Glucose Oxidase ◽  
Organic Semiconductors ◽  
Theoretical Investigation ◽  
Glucose Biosensors ◽  
Carrier Transfer ◽  
Amperometric Glucose Biosensors ◽  
Transfer Mechanisms

We have evaluated charge transfer between enzyme glucose oxidase (GOx) and organic semiconductors, both of which were applied in the design of amperometric glucose biosensors.

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