Selective Photodynamic Inactivation of Bacterial Cells over Mammalian Cells by New Triarylmethanes

Langmuir ◽  
2014 ◽  
Vol 30 (48) ◽  
pp. 14573-14580 ◽  
Author(s):  
Ke Li ◽  
Wanhua Lei ◽  
Guoyu Jiang ◽  
Yuanjun Hou ◽  
Baowen Zhang ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Photodynamic Inactivation ◽  
Bacterial Cells

Cationic benzylidene cyclopentanone photosensitizers for selective photodynamic inactivation of bacteria over mammalian cells

RSC Advances ◽  
2015 ◽  
Vol 5 (69) ◽  
pp. 56067-56074 ◽  
Author(s):  
Yanyan Fang ◽  
Tianlong Liu ◽  
Qianli Zou ◽  
Yuxia Zhao ◽  
Feipeng Wu
Keyword(s):  
Mammalian Cells ◽  
Photodynamic Inactivation ◽  
Bacterial Cells ◽  
Inactivation Of Bacteria

Cationic modified benzylidene cyclopentanone photosensitizers selectively photo-inactivate bacterial cells over mammalian cells.

scholarly journals The Use of Bacteriophage for Detection and Biocontrol of Foodborne Pathogen

2018 ◽  
Vol 28 (1) ◽  
pp. 33
Author(s):  
Tati Ariyanti
Keyword(s):  
Mammalian Cells ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Foodborne Pathogen ◽  
Foodborne Disease ◽  
Bacterial Cells ◽  
Detection Tool ◽  
Specific Receptors ◽  
Antibiotics Detection ◽  
Potential Use

Bacteriophages are viruses that have ability to attack bacterial cells in specific receptors, infect, multiply in bacterial cells and eventually lyse bacterial cells. This unique bacteriophage character is highly beneficial because it is harmless to mammalian cells and does not interfere with natural microbes. Bacteriophages are easy to obtain because they are widespread in the environment such as soil, water, animal, and farm waste or food. This paper describes the potential use of bacteriophages to detect pathogen and foodborne pathogen biocontrol. Bacteriophages are very potential to control the growth of pathogenic bacteria both in food industry and environment. Bacteriophages act as antibiotics, detection tool for pathogenic bacteria in the food chain, food biopreservative from pathogen bacteria contamination, and foodborne disease prevention. Although research on bacteriophage in Indonesia has not been widely reported, research on bacteriophage utilization is being carried on.

Role of an endoplasmic reticulum Ca2+-independent phospholipase A2 in oxidant-induced renal cell death

2002 ◽  
Vol 283 (3) ◽  
pp. F492-F498 ◽  
Author(s):  
Brian S. Cummings ◽  
Jane McHowat ◽  
Rick G. Schnellmann
Keyword(s):  
Lipid Peroxidation ◽  
Endoplasmic Reticulum ◽  
Subcellular Localization ◽  
Mammalian Cells ◽  
Cumene Hydroperoxide ◽  
Physiological Role ◽  
Antimycin A ◽  
Bacterial Cells ◽  
Tert Butylhydroperoxide ◽  
Membrane Bound

Phospholipase A2(PLA2) hydrolyzes the sn-2 ester bond in phospholipids, releasing a fatty acid and a lysophospholipid. Recently, a novel 85-kDa membrane-bound-Ca2+-independent PLA2 (iPLA2) was identified in insect and bacterial cells transfected with candidate PLA2 sequences. However, few data exist demonstrating a membrane-bound-iPLA2 in mammalian cells, its subcellular localization, or its physiological role. Herein, we demonstrate the expression of an 85-kDa endoplasmic reticulum (ER)-Ca2+-iPLA2 (ER-iPLA2) in rabbit renal proximal tubule cells (RPTC) that is plasmalogen selective and is inhibited by the specific Ca2+-iPLA2inhibitor bromoenol lactone (BEL). RPTC exposed to tert-butylhydroperoxide for 24 h exhibited 20% oncosis compared with 2% in controls. Inhibition of ER-iPLA2 with BEL before tert-butylhydroperoxide exposure resulted in 50% oncosis. To determine whether this effect was common to oxidants, we tested the ability of BEL to potentiate oncosis induced by cumene hydroperoxide, menadione, duraquinone, cisplatin, and the nonoxidant antimycin A. All oxidants tested produced oncosis after 24 h, and prior inhibition of ER-iPLA2 potentiated oncosis at least twofold. In contrast, inhibition of ER-iPLA2 did not alter antimycin A-induced oncosis. Lipid peroxidation increased from 1.4- to 5.2-fold in RPTC treated with BEL before oxidant exposure, whereas no change was seen in antimycin A-treated RPTC. These results are the first to demonstrate the expression and subcellular localization of an ER-iPLA2. These results also suggest that ER-iPLA2 functions to protect against oxidant-induced lipid peroxidation and oncosis.

Attachment of Salmonella to mammalian cells in vitro

1983 ◽  
Vol 29 (12) ◽  
pp. 1731-1735 ◽  
Author(s):  
Clifford S. Mintz ◽  
Dean O. Cliver ◽  
R. H. Deibel
Keyword(s):  
Cell Lines ◽  
Mammalian Cells ◽  
Culture Cell ◽  
Intestinal Cell ◽  
Tissue Culture Cell ◽  
Bacterial Cells ◽  
Intestinal Cell Line ◽  
Hela Cell Lines ◽  
High Concentrations

The attachment of Salmonella typhimurium strain PHL67342 to several mammalian tissue culture cell lines was investigated. Strain PHL67342 failed to attach in significant numbers to the Buffalo green monkey (BGM), swine testicular (ST), and HeLa cell lines. Significant attachment was observed with the Henle intestinal cell line. Log-phase cells of strain PHL67342 attached in greatest numbers to the Henle cells after 45 min of incubation at 37 °C. Attachment to the Henle cells was not affected by D-mannose or D-galactose, but was markedly inhibited by high concentrations of alpha-methyl-D-mannoside. Also, Salmonella lipopolysaccharide had no effect on the attachment of strain PHL67342 to the Henle cells. Fimbriae were not detected on the bacterial cells used in the adherence experiments. These results suggest that some bacterial factor(s) other than fimbriae and lipopolysaccharide mediate the attachment of strain PHL67342 to the Henle cells.

Cellulose–Ethylenediaminetetraacetic Acid Conjugates Protect Mammalian Cells from Bacterial Cells

2013 ◽  
Vol 14 (4) ◽  
pp. 1054-1062 ◽  
Author(s):  
Jie Luo ◽  
Wei Lv ◽  
Ying Deng ◽  
Yuyu Sun
Keyword(s):  
Mammalian Cells ◽  
Ethylenediaminetetraacetic Acid ◽  
Bacterial Cells

scholarly journals Efficient and cost-effective bacterial mRNA sequencing from low input samples through ribosomal RNA depletion

2020 ◽  
Author(s):  
Chatarin Wangsanuwat ◽  
Kellie A. Heom ◽  
Estella Liu ◽  
Michelle A. O’Malley ◽  
Siddharth S. Dey
Keyword(s):  
Mammalian Cells ◽  
Expression Profiles ◽  
Bacterial Species ◽  
Gene Expression Profiles ◽  
Cost Effective ◽  
Wide Distribution ◽  
23S Rrna ◽  
Bacterial Cells ◽  
Low Input ◽  
Mrna Sequencing

AbstractRNA sequencing is a powerful approach to quantify the genome-wide distribution of mRNA molecules in a population to gain deeper understanding of cellular functions and phenotypes. However, unlike eukaryotic cells, mRNA sequencing of bacterial samples is more challenging due to the absence of a poly-A tail that typically enables efficient capture and enrichment of mRNA from the abundant rRNA molecules in a cell. Moreover, bacterial cells frequently contain 100-fold lower quantities of RNA compared to mammalian cells, which further complicates mRNA sequencing from non-cultivable and non-model bacterial species. To overcome these limitations, we report EMBR-seq (Enrichment of mRNA by Blocked rRNA), a method that efficiently depletes 5S, 16S and 23S rRNA using blocking primers to prevent their amplification, resulting in greater than 80% of the sequenced RNA molecules from an E. coli culture deriving from mRNA. We demonstrate that this increased efficiency provides a deeper view of the transcriptome without introducing technical amplification-induced biases. Moreover, compared to recent methods that employ a large array of oligonucleotides to deplete rRNA, EMBR-seq uses a single oligonucleotide per rRNA, thereby making this new technology significantly more cost-effective, especially when applied to varied bacterial species. Finally, compared to existing commercial kits for bacterial rRNA depletion, we show that EMBR-seq can be used to successfully quantify the transcriptome from more than 500-fold lower starting total RNA. Thus, EMBR-seq provides an efficient and cost-effective approach to quantify global gene expression profiles from low input bacterial samples.

scholarly journals Bacterial envelope damage inflicted by bioinspired nanospikes grown in a hydrogel

2020 ◽  
Author(s):  
Sandra L. Arias ◽  
Joshua Devorkin ◽  
Jessica C. Spear ◽  
Ana Civantos ◽  
Jean Paul Allain
Keyword(s):  
Mechanical Properties ◽  
Antimicrobial Activity ◽  
Cell Wall ◽  
Mammalian Cells ◽  
Cell Shape ◽  
Rational Design ◽  
Bacterial Cells ◽  
Nanoscale Structures ◽  
Gram Positive Bacteria ◽  
Tissue Restoration

AbstractDevice-associated infections are one of the deadliest complications accompanying the use of biomaterials, and despite recent advances in the development of anti-biofouling strategies, biomaterials that exhibit both functional tissue restoration and antimicrobial activity have been challenging to achieve. Here, we report the fabrication of bio-inspired bactericidal nanospikes in bacterial cellulose and investigate the mechanism underlying this phenomenon. We demonstrate these structures affects preferentially stiff membranes like those in Gram-positive bacteria, but exhibit cytocompatibility towards mammalian cells, a requisite for tissue restoration. We also reveal the bactericidal activity of the nanospikes is due to a pressure-induced mechanism, which depends on the cell’s adherence time, nanospike’s geometry and spacing, cell shape, and mechanical properties of the cell wall. Our findings provide a better understanding of the mechanobiology of bacterial cells at the interface with nanoscale structures, which is fundamental for the rational design bactericidal topographies.

scholarly journals Current State of Biosensors Development and their Application in Foodborne Pathogen Detection

2021 ◽  
Author(s):  
LUPING XU ◽  
Xingjian Bai ◽  
Arun K. Bhunia
Keyword(s):  
Mammalian Cells ◽  
Pathogen Detection ◽  
Safety Concern ◽  
Point Of Care ◽  
Foodborne Pathogen ◽  
Disease Outbreaks ◽  
High Specificity ◽  
Bacterial Cells ◽  
Outbreak Investigations ◽  
Foodborne Pathogen Detection

Foodborne disease outbreaks continue to be a major public health and food safety concern. Ensuring the safety of food prior to retail distribution by testing products promptly can protect consumers from foodborne diseases. F ast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored in the past few years to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1-100 bacterial cells or pico-nanogram levels of toxins). Besides, advancement in mammalian cells and bacteriophage-based sensors led to their ability to detect not only low levels of pathogens but also to differentiate live from dead ones. Combining different biotechnology platforms enabled practical utility and application of biosensors in foodborne pathogen detection. However, further rigorous testing of biosensors from complex food matrices is needed to ensure their utility in point-of-care need and for outbreak investigations.

scholarly journals Applications of In-Cell NMR in Structural Biology and Drug Discovery

2019 ◽  
Vol 20 (1) ◽  
pp. 139 ◽  
Author(s):  
CongBao Kang
Keyword(s):  
Drug Discovery ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Structural Information ◽  
Living Cells ◽  
Bacterial Cells ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Post Translational Modifications

In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information of a target at an atomic level under physiological conditions and a full view of the conformational changes of a protein caused by ligand binding, post-translational modifications or protein–protein interactions in living cells. Previous in-cell NMR studies have focused on proteins that were overexpressed in bacterial cells and isotopically labeled proteins injected into oocytes of Xenopus laevis or delivered into human cells. Applications of in-cell NMR in probing protein modifications, conformational changes and ligand bindings have been carried out in mammalian cells by monitoring isotopically labeled proteins overexpressed in living cells. The available protocols and successful examples encourage wide applications of this technique in different fields such as drug discovery. Despite the challenges in this method, progress has been made in recent years. In this review, applications of in-cell NMR are summarized. The successful applications of this method in mammalian and bacterial cells make it feasible to play important roles in drug discovery, especially in the step of target engagement.

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