scholarly journals Dynamic Measurements of Membrane Insertion Potential of Synthetic Cell Penetrating Peptides

Langmuir ◽  
2013 ◽  
Vol 29 (49) ◽  
pp. 15336-15349 ◽  
Author(s):  
Nabil A. Alhakamy ◽  
Anubhav Kaviratna ◽  
Cory J. Berkland ◽  
Prajnaparamita Dhar
Keyword(s):  
Cell Penetrating Peptides ◽  
Membrane Insertion ◽  
Dynamic Measurements ◽  
Synthetic Cell ◽  
Cell Penetrating

scholarly journals Membrane Insertion Potential of Synthetic Cell Penetrating Peptides

2014 ◽  
Vol 106 (2) ◽  
pp. 97a
Author(s):  
Nabil A. Alhakamy ◽  
Anubhav Kaviratna ◽  
Cory Berkland ◽  
Prajnaparamita Dhar
Keyword(s):  
Cell Penetrating Peptides ◽  
Membrane Insertion ◽  
Synthetic Cell ◽  
Cell Penetrating

Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier

2010 ◽  
Vol 17 (2) ◽  
pp. 164-167 ◽  
Author(s):  
Shinya Kida ◽  
Yusuke Eto ◽  
Yasuo Yoshioka ◽  
Shinsaku Nakagawa ◽  
Koichi Kawasaki ◽  
...  
Keyword(s):  
Adenovirus Vector ◽  
Cell Penetrating Peptides ◽  
Synthetic Cell ◽  
Cell Penetrating

Tuning the properties of a novel short cell-penetrating peptide by intramolecular cyclization with a triazole bridge

2016 ◽  
Vol 52 (11) ◽  
pp. 2261-2264 ◽  
Author(s):  
M. Horn ◽  
F. Reichart ◽  
S. Natividad-Tietz ◽  
D. Diaz ◽  
I. Neundorf
Keyword(s):  
Intramolecular Cyclization ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Membrane Insertion ◽  
Short Cell ◽  
Cell Penetrating

Cyclic versus linear: cyclic triazole-bridged cell-penetrating peptides are optimally arranged within the membrane, thus at the same time inducing suitable DNA complexation and successful peptide membrane insertion.

scholarly journals Effect of Synthetic Cell-Penetrating Peptides on TrkA Activity in PC12 Cells

2008 ◽  
Vol 106 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Munetaka Hirose ◽  
Mayumi Takatori ◽  
Yoshihiro Kuroda ◽  
Mineo Abe ◽  
Eri Murata ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Cell Penetrating Peptides ◽  
Synthetic Cell ◽  
Cell Penetrating

scholarly journals Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide

2019 ◽  
Vol 20 (18) ◽  
pp. 4441
Author(s):  
Marie-Lise Jobin ◽  
Lydie Vamparys ◽  
Romain Deniau ◽  
Axelle Grélard ◽  
Cameron Mackereth ◽  
...  
Keyword(s):  
Eukaryotic Cell ◽  
Cell Penetrating Peptides ◽  
Membrane Insertion ◽  
Antitumor Activities ◽  
Dynamic Simulations ◽  
Zwitterionic Lipids ◽  
Cell Penetrating ◽  
High Resolution Structure ◽  
And Behavior ◽  
Intracellular Milieu

Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacterial and antitumor activities. Using complementary approaches, including NMR spectroscopy, fluorescence spectroscopy, circular dichroism, and molecular dynamic simulations, we determined the high-resolution structure of RW16 and measured its membrane insertion and orientation properties into zwitterionic membranes. Altogether, these results contribute to explaining the versatile properties of this peptide toward zwitterionic lipids.

scholarly journals Cell-Penetrating CEBPB and CEBPD Leucine Zipper Decoys as Broadly Acting Anti-Cancer Agents

Cancers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2504
Author(s):  
Qing Zhou ◽  
Xiotian Sun ◽  
Nicolas Pasquier ◽  
Parvaneh Jefferson ◽  
Trang T. T. Nguyen ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transcriptional Activation ◽  
Leucine Zipper ◽  
Cell Penetrating Peptides ◽  
Multiple Tumor ◽  
Synthetic Cell ◽  
Anti Cancer ◽  
Cell Penetrating

Transcription factors are key players underlying cancer formation, growth, survival, metastasis and treatment resistance, yet few drugs exist to directly target them. Here, we characterized the in vitro and in vivo anti-cancer efficacy of novel synthetic cell-penetrating peptides (Bpep and Dpep) designed to interfere with the formation of active leucine-zipper-based dimers by CEBPB and CEBPD, transcription factors implicated in multiple malignancies. Both peptides similarly promoted apoptosis of multiple tumor lines of varying origins, without such effects on non-transformed cells. Combined with other treatments (radiation, Taxol, chloroquine, doxorubicin), the peptides acted additively to synergistically and were fully active on Taxol-resistant cells. The peptides suppressed expression of known direct CEBPB/CEBPD targets IL6, IL8 and asparagine synthetase (ASNS), supporting their inhibition of transcriptional activation. Mechanisms by which the peptides trigger apoptosis included depletion of pro-survival survivin and a required elevation of pro-apoptotic BMF. Bpep and Dpep significantly slowed tumor growth in mouse models without evident side effects. Dpep significantly prolonged survival in xenograft models. These findings indicate the efficacy and potential of Bpep and Dpep as novel agents to treat a variety of cancers as mono- or combination therapies.

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