Surface Charge Dependent Nanoparticle Disruption and Deposition of Lipid Bilayer Assemblies

Langmuir ◽  
2012 ◽  
Vol 28 (50) ◽  
pp. 17396-17403 ◽  
Author(s):  
Xiaoyin Xiao ◽  
Gabriel A. Montaño ◽  
Thayne L. Edwards ◽  
Amy Allen ◽  
Komandoor E. Achyuthan ◽  
...  
Keyword(s):  
Surface Charge ◽  
Lipid Bilayer

scholarly journals Controlling the surface charge of simple viruses

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0255820
Author(s):  
A. L. Duran-Meza ◽  
M. V. Villagrana-Escareño ◽  
J. Ruiz-García ◽  
C. M. Knobler ◽  
W. M. Gelbart
Keyword(s):  
Ionic Strength ◽  
Surface Charge ◽  
Lipid Bilayer ◽  
Colloidal Particles ◽  
Plant Viruses ◽  
Brome Mosaic Virus ◽  
Host Cells ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

The vast majority of plant viruses are unenveloped, i.e., they lack a lipid bilayer that is characteristic of most animal viruses. The interactions between plant viruses, and between viruses and surfaces, properties that are essential for understanding their infectivity and to their use as bionanomaterials, are largely controlled by their surface charge, which depends on pH and ionic strength. They may also depend on the charge of their contents, i.e., of their genes or–in the instance of virus-like particles–encapsidated cargo such as nucleic acid molecules, nanoparticles or drugs. In the case of enveloped viruses, the surface charge of the capsid is equally important for controlling its interaction with the lipid bilayer that it acquires and loses upon leaving and entering host cells. We have previously investigated the charge on the unenveloped plant virus Cowpea Chlorotic Mottle Virus (CCMV) by measurements of its electrophoretic mobility. Here we examine the electrophoretic properties of a structurally and genetically closely related bromovirus, Brome Mosaic Virus (BMV), of its capsid protein, and of its empty viral shells, as functions of pH and ionic strength, and compare them with those of CCMV. From measurements of both solution and gel electrophoretic mobilities (EMs) we find that the isoelectric point (pI) of BMV (5.2) is significantly higher than that of CCMV (3.7), that virion EMs are essentially the same as those of the corresponding empty capsids, and that the same is true for the pIs of the virions and of their cleaved protein subunits. We discuss these results in terms of current theories of charged colloidal particles and relate them to biological processes and the role of surface charge in the design of new classes of drug and gene delivery systems.

scholarly journals Detection of the electrical surface charge induced by treatment of the membrane lipid bilayer of human erythrocytes.

1982 ◽  
Vol 7 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Akira Tamura ◽  
Kunihiko Morita ◽  
Tatsuzo Fujii ◽  
Kiyohide Kojima
Keyword(s):  
Surface Charge ◽  
Lipid Bilayer ◽  
Membrane Lipid ◽  
Human Erythrocytes ◽  
Membrane Lipid Bilayer

Surface Charge and the Association of Liposomes with Colon Carcinoma Cells

2001 ◽  
Vol 56 (9-10) ◽  
pp. 872-877 ◽  
Author(s):  
Dagmara Baczynska ◽  
Katarzyna Widerak ◽  
Maciej Ugorski ◽  
Marek Langner
Keyword(s):  
Surface Charge ◽  
Lipid Bilayer ◽  
Electrostatic Interactions ◽  
Cationic Lipid ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Lipid Surface ◽  
Positively Charged ◽  
Effect Of Surface

Abstract Interaction between the plasma membrane and aggregate lipid surface determines how efficiently the encapsulated drug will be delivered into the cell. Electrostatic interactions are one of the main forces affecting liposome and aggregate association with the charged cell surface. In this study, the effect of surface charge on the association of liposomes with human colon CX-1.1 cancer cells was studied. When phosphatidylserine was incorporated into a lipid bilayer, the amount of liposomes associated with cells tended to increase along with the amount of negatively charged lipid present in the liposomal lipid bilayer. When the cationic lipid dioleoyl-1,2-diacyl-3-trimethylammonium-propane (DOTAP) was included into the liposome formula, their uptake by the cells was also increased. Maximum binding occurred when the amount of positively charged lipids in liposomes was about 10 mol% of lipids.

scholarly journals Hydration Forces Dominate Surface Charge Dependent Lipid Bilayer Interactions under Physiological Conditions

2021 ◽  
pp. 9248-9252
Author(s):  
Valentina Wieser ◽  
Laura L. E. Mears ◽  
Robert D. Barker ◽  
Hsiu-Wei Cheng ◽  
Markus Valtiner
Keyword(s):  
Surface Charge ◽  
Lipid Bilayer ◽  
Physiological Conditions ◽  
Hydration Forces
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