Surface Charge Dependence of Polyelectrolyte-Induced Domain Size and Composition in Lipid Bilayer Membranes

1998 ◽  
Vol 102 (45) ◽  
pp. 9091-9100 ◽  
Author(s):  
Kevin J. Crowell ◽  
Peter M. Macdonald
Keyword(s):  
Surface Charge ◽  
Lipid Bilayer ◽  
Domain Size ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Charge Dependence

Polyelectrolyte-induced domains in lipid bilayer membranes: the deuterium NMR perspective

1998 ◽  
Vol 76 (2-3) ◽  
pp. 452-464 ◽  
Author(s):  
Peter M Macdonald ◽  
Kevin J Crowell ◽  
Carla M Franzin ◽  
Peter Mitrakos ◽  
Darlene J Semchyschyn
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Domain Size ◽  
Complete Analysis ◽  
Deuterium Nmr ◽  
Domain Formation ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
H Nmr ◽  
Oppositely Charged

Domain formation in lipid bilayer membranes can occur through electrostatic interactions between charged lipids and oppositely charged polyelectrolytes, such as proteins or polynucleic acids. This review describes a novel method for examining such domains in lipid bilayers, based on 2H NMR spectroscopy. The 2H NMR spectrum of choline-deuterated phosphatidylcholine is sensitive to, and reports on, lipid bilayer surface charge. When a charged lipid bilayer is exposed to an oppositely charged polyelectrolyte, the latter binds electrostatically to the bilayer surface and attracts charged lipids into its vicinity. The resulting inhomogeneous charge distribution produces overlapping 2H NMR subspectra arising from phosphatidylcholine within charge-enriched versus charge-depleted regions. Such spectral details as the quadrupolar splittings and the relative intensities of the subspectra permit a complete analysis of the domain composition, size, and, within limits, lifetime. Using 2H NMR, domain formation in lipid bilayer membranes can be observed with both cationic and anionic polyelectrolytes, whether of natural or synthetic origin. Domain size and composition prove to be sensitive to the detailed chemical structure of both the polyelectrolyte and the charged lipids. Within the domains there is always a stoichiometric anion/cation binding ratio, indicating that the polyelectrolyte lies flat on the membrane surface. The amount of phosphatidylcholine within the domain varies as a function of its statistical availability, in accordance with the predictions of a recent thermodynamic model of domain formation. When the molecular weight of the polyelectrolyte is varied, the domain size alters in accordance with the predictions of classical polymer physics. As expected for a predominantly electrostatic phenomenon, the observed domains dissipate at high ionic strength.Key words: electrostatic domains, polyelectrolytes, lipid bilayers, deuterium NMR.

Formation of asymmetrical planar lipid bilayer membranes from characterized monolayers

1983 ◽  
Vol 7 (4) ◽  
pp. 299-310 ◽  
Author(s):  
Pierre Tancrède ◽  
Paul Paquin ◽  
André Houle ◽  
Roger M. Leblanc
Keyword(s):  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

Calcium-induced reversible assembly of phosphorylated amphiphile within lipid bilayer membranes

2021 ◽  
Author(s):  
Yusuke Shimizu ◽  
Kohei Sato ◽  
Kazushi Kinbara
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayer ◽  
Phenylene Ethynylene ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

Inspired by calcium-induced reversible assembly and disassembly of membrane proteins found in nature, here we developed a phosphorylated amphiphile (PA) that contains an oligo(phenylene-ethynylene) unit as a hydrophobic unit and...

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