Layer by Layer Self-Assembled Polyelectrolyte Multilayers with Embedded Phospholipid Vesicles Obtained by Spraying: Integrity of the Vesicles

Langmuir ◽  
2005 ◽  
Vol 21 (17) ◽  
pp. 7854-7859 ◽  
Author(s):  
M. Michel ◽  
A. Izquierdo ◽  
G. Decher ◽  
J.-C. Voegel ◽  
P. Schaaf ◽  
...  
Langmuir ◽  
2004 ◽  
Vol 20 (12) ◽  
pp. 4835-4839 ◽  
Author(s):  
Marc Michel ◽  
Dominique Vautier ◽  
Jean-Claude Voegel ◽  
Pierre Schaaf ◽  
Vincent Ball

2010 ◽  
Vol 649 (1-2) ◽  
pp. 110-118 ◽  
Author(s):  
Mario Tagliazucchi ◽  
Lucila P. Méndez De Leo ◽  
Alejandro Cadranel ◽  
Luis M. Baraldo ◽  
Edgar Völker ◽  
...  

Langmuir ◽  
2006 ◽  
Vol 22 (5) ◽  
pp. 2358-2364 ◽  
Author(s):  
Marc Michel ◽  
Youri Arntz ◽  
Guillaume Fleith ◽  
Julien Toquant ◽  
Youssef Haikel ◽  
...  

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 133
Author(s):  
Yu-Che Cheng ◽  
Shu-Lin Guo ◽  
Kun-Da Chung ◽  
Wei-Wen Hu

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through layer-by-layer (LbL) assembly to form polyelectrolyte multilayers (PEMs). Although these macromolecules can be released for transfection purposes, their entanglement only allows partial delivery. Therefore, how to efficiently deliver immobilized genes from PEMs remains a challenge. In this study, we attempt to facilitate their delivery through the pretreatment of the external electrical field. Multilayers of polyethylenimine (PEI) and DNA were deposited onto conductive polypyrrole (PPy), which were placed in an aqueous environment to examine their release after electric field pretreatment. Only the electric field perpendicular to the substrate with constant voltage efficiently promoted the release of PEI and DNA from PEMs, and the higher potential resulted in the more releases which were enhanced with treatment time. The roughness of PEMs also increased after electric field treatment because the electrical field not only caused electrophoresis of polyelectrolytes and but also allowed electrochemical reaction on the PPy electrode. Finally, the released DNA and PEI were used for transfection. Polyplexes were successfully formed after electric field treatment, and the transfection efficiency was also improved, suggesting that this electric field pretreatment effectively assists gene delivery from PEMs and should be beneficial to regenerative medicine application.


Langmuir ◽  
2002 ◽  
Vol 18 (10) ◽  
pp. 4020-4029 ◽  
Author(s):  
Erica S. Forzani ◽  
Marcelo Otero ◽  
Manuel A. Pérez ◽  
Manuel López Teijelo ◽  
Ernesto J. Calvo

2017 ◽  
Vol 13 (4) ◽  
pp. 1423-1433 ◽  
Author(s):  
Xilin Liu ◽  
Fei Han ◽  
Peng Zhao ◽  
Chao Lin ◽  
Xuejun Wen ◽  
...  

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