Electrophoretic Behavior of Complexes between Sodium Dextran Sulfate and Cationic Surfactants

Langmuir ◽  
2000 ◽  
Vol 16 (20) ◽  
pp. 7629-7633 ◽  
Author(s):  
Yoshiko Moriyama ◽  
Kunio Takeda ◽  
Kiyofumi Murakami
Keyword(s):  
Cationic Surfactants ◽  
Dextran Sulfate ◽  
Electrophoretic Behavior ◽  
Sodium Dextran Sulfate

Assembly of particles from southern bean mosaic virus and sowbane mosaic virus components

1977 ◽  
Vol 55 (16) ◽  
pp. 2274-2277 ◽  
Author(s):  
J. H. Tremaine ◽  
W. P. Ronald
Keyword(s):  
Mosaic Virus ◽  
Dextran Sulfate ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Divalent Metal ◽  
Spherical Particles ◽  
Divalent Metal Ions ◽  
Southern Bean Mosaic Virus ◽  
Sodium Dextran Sulfate ◽  
Protein Components

Southern bean mosaic virus (SBMV) and sowbane mosaic virus (SoMV) were each dissociated into RNA and protein components in neutral pH buffers containing ethylenediaminetetraacetic acid and 1 M NaCl. The assembly of SBMV particles and of SoMV RNA in SBMV protein was accomplished by dialysis of the virus components into low molarity buffers containing divalent metal ions. Some of these particles were stable in 1% sodium dodecyl sulfate (SDS) and had sedimentation and electrophoretic properties identical with untreated virus. Spherical particles were also assembled with either SBMV or SoMV RNA in SoMV protein and with sodium dextran sulfate in either SBMV or SoMV protein, but these particles were not stable in 1% SDS.

scholarly journals The oxysterol synthesizing enzyme CH25H contributes to the development of intestinal fibrosis

2018 ◽  
Author(s):  
T. Raselli ◽  
A. Wyss ◽  
M.N. Gonzalez Alvarado ◽  
B. Weder ◽  
C. Mamie ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dextran Sulfate ◽  
Biological Processes ◽  
Intestinal Fibrosis ◽  
Oxidized Cholesterol ◽  
Sodium Dextran Sulfate ◽  
Transplant Model ◽  
And Oxidative Stress ◽  
Colitis Model ◽  
Transplantation Model

AbstractIntestinal fibrosis and stenosis are common complications of Crohn’s disease (CD), frequently requiring surgery. Anti-inflammatory strategies can only partially prevent fibrosis; hence, anti-fibrotic therapies remain an unmet clinical need. Oxysterols are oxidized cholesterol derivatives, with important roles in various biological processes. The enzyme cholesterol 25-hydroxylase (CH25H) converts cholesterol to 25-hydroxycholesterol (25-HC), which modulates immune responses and oxidative stress. In human intestinal samples from CD patients we found a strong correlation of CH25H mRNA expression with the expression of fibrosis markers. We demonstrate reduced intestinal fibrosis in mice deficient for the CH25H enzyme using the sodium dextran sulfate (DSS)-induced chronic colitis model. Additionally, using a heterotopic transplantation model of intestinal fibrosis, we demonstrate reduced collagen deposition and lower concentrations of hydroxyproline in CH25H knockouts. In the heterotopic transplant model, CH25H was expressed in fibroblasts. Taken together, our findings indicate an involvement of oxysterol synthesis in the pathogenesis of intestinal fibrosis.

Die Verteilung von Adenovirus-Komponenten im Zweiphasensystem wäßriger polymerer Lösungen

1968 ◽  
Vol 23 (1) ◽  
pp. 90-93 ◽  
Author(s):  
W. A. K. Schmidt
Keyword(s):  
Polyethylene Glycol ◽  
Dextran Sulfate ◽  
Adenovirus Type ◽  
Two Phase ◽  
Sodium Dextran Sulfate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Two Phases

The distribution of antigen components of adenovirus type 26 in aqueous two-phase systems of sodium dextran sulfate and polyethylene glycol is described. Depending on composition and NaCl molarity of the system, the infectious particles and the soluble haemagglutinin were either concentrated in one phase or distributed in two phases and separated from one another. The complement-fixing group antigen could not be determined, since the polymers interfered extensively with the CF test.

Electrophoretic Light Scattering Study of Sodium Dextran Sulfate–Lysozyme Complex

1996 ◽  
Vol 179 (1) ◽  
pp. 249-254 ◽  
Author(s):  
Koji Yamaguchi ◽  
Kazuaki Hachiya ◽  
Yoshiko Moriyama ◽  
Kunio Takeda
Keyword(s):  
Light Scattering ◽  
Dextran Sulfate ◽  
Electrophoretic Light Scattering ◽  
Sodium Dextran Sulfate ◽  
Scattering Study
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