Carboxylesterase-Mediated Transesterification of Meperidine (Demerol) and Methylphenidate (Ritalin) in the Presence of [2H6]Ethanol: Preliminary in Vitro Findings Using a Rat Liver Preparation

1997 ◽  
Vol 86 (12) ◽  
pp. 1494-1496 ◽  
Author(s):  
James A. Bourland ◽  
Debra K. Martin ◽  
Michael Mayersohn
Keyword(s):  
Rat Liver ◽  
Liver Preparation

The effects of cofactor and species differences on the in vitro metabolism of propiophenone and phenylacetone

1981 ◽  
Vol 59 (2) ◽  
pp. 195-201 ◽  
Author(s):  
R. T. Coutts ◽  
D. R. Prelusky ◽  
G. R. Jones
Keyword(s):  
Rat Liver ◽  
In Vitro Metabolism ◽  
Metabolic Route ◽  
Liver Preparation ◽  
Carbonyl Reduction ◽  
Alcohol Dehydrogenation ◽  
Liver Homogenates ◽  
A Minor ◽  
Minor Extent

In vitro metabolism of the aromatic ketone propiophenone and its nonaromatic isomer phenylacetone was studied using fortified 12 000 × g supernatants of liver homogenates from rat and rabbit. Reduction to the corresponding alcohols was the major metabolic route observed, although aliphatic C-hydroxylation and alcohol dehydrogenation also occurred. Marked differences were observed in the amounts of carbonyl reduction of the substrates, which was dependant on the species as well as the cofactor employed. Using rat liver preparation, phenylacetone was reduced to 1-phenyl-2-propanol much more efficiently with an NADH-fortified system than when NADPH was used whereas in rabbit, extensive reduction occurred in the presence of either cofactor. Reduction of propiophenone to 1-phenyl-1-propanol by rat liver preparation was slightly greater in the presence of NADH than with NADPH; the converse was observed in rabbit.Aliphatic hydroxylation of propiophenone to 2-hydroxy-1-phenyl-1-propamine was also a significant metabolic pathway in both species, with NADPH being the more efficient cofactor, but C-1 hydroxylation of phenylacetone to 1-hydroxy-1-phenyl-2-propanone occurred only to a minor extent. Small amounts of 1-phenyl-1,2-propanedione, as well as both erythro and threo isomers of 1-phenyl-1,2-propanediol, were also identified as metabolites in both species. Similar metabolic studies were carried out on the alcohols 1-phenyl-1-propanol and 1-phenyl-2-propanol and again the nature and quantities of metabolites isolated showed both species and cofactor dependancies.

scholarly journals The catabolism of cholesterol in vitro. Formation of 3α,7α,12α-trihydroxy-5β-cholestanoic acid from cholesterol by rat liver

1969 ◽  
Vol 114 (1) ◽  
pp. 1-3 ◽  
Author(s):  
D. Mendelsohn ◽  
L. Mendelsohn
Keyword(s):  
Gall Bladder ◽  
Bile Acids ◽  
Rat Liver ◽  
Specific Radioactivity ◽  
Liver Preparation

1. Both 25-d- and 25-l-3α,7α,12α-trihydroxy-5β-cholestanoic acid were isolated from the gall-bladder bile of Crocodylus niloticus. 2. The catabolism of cholesterol to 25-d- and 25-l-3α,7α,12α-trihydroxy-5β-cholestanoic acid respectively was studied by using a rat liver preparation in vitro. The results show that rat liver can metabolize cholesterol to both forms of 3α,7α,12α-trihydroxy-5β-cholestanoic acid. However, a preference was noted for the formation from [4−14C]cholesterol of 3α,7α,12α-trihydroxy-5β-cholestanoic acid (25-d), which was isolated from the incubations with a specific radioactivity about four times that of 3α,7α,12α-trihydroxy-5β-cholestanoic acid (25-l). 3. The results indicate that 3α,7α,12α-trihydroxy-5β-cholestanoic acid is a normal intermediate in the biosynthesis of bile acids from cholesterol in the rat.

scholarly journals The dream team meets liver perfusion: From gluconeogenesis to hepatic physiology

2012 ◽  
Vol 34 (1) ◽  
pp. 35-37
Author(s):  
John T. Brosnan ◽  
Margaret E. Brosnan
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
Biochemical Journal ◽  
Liver Preparation ◽  
Classic Paper

This Biochemical Journal ‘Classic Paper’ article describes the development in the Krebs laboratory of a simple and robust procedure for perfusion of the rat liver. Although initially designed for the study of gluconeogenesis, the procedure has been widely used for a variety of studies which require a physiological in vitro liver preparation.

The Effect of Adrenaline Pretreatment on the In Vitro Generation of 3,5,3′-Triiodothyronine and 3,3′,5′-Triiodothyronine (Reverse T3) in Rat Liver Preparation

1984 ◽  
Vol 16 (09) ◽  
pp. 471-474 ◽  
Author(s):  
A. Nauman ◽  
S. Porta ◽  
U. Bardowska ◽  
K. Fiedorowicz ◽  
A. Sadjak ◽  
...  
Keyword(s):  
Rat Liver ◽  
Reverse T3 ◽  
Liver Preparation

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

Insulin-like action of proinsulin on rat liver carbohydrate metabolism in vitro

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 415-419 ◽  
Author(s):  
I. Probst ◽  
H. Hartmann ◽  
K. Jungermann ◽  
W. Creutzfeldt
Keyword(s):  
Carbohydrate Metabolism ◽  
Rat Liver
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