Modeling the Electrostatic Signature of Single Enzyme Activity

2010 ◽  
Vol 114 (9) ◽  
pp. 3330-3333 ◽  
Author(s):  
Landon Prisbrey ◽  
Guenter Schneider ◽  
Ethan Minot
Keyword(s):  
Enzyme Activity ◽  
Single Enzyme

scholarly journals Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts

ACS Catalysis ◽  
2016 ◽  
Vol 6 (12) ◽  
pp. 8313-8320 ◽  
Author(s):  
Enno Kätelhön ◽  
Lior Sepunaru ◽  
Arkady A. Karyakin ◽  
Richard G. Compton
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

Regulation of Chloramphenicol Synthesis in Streptomyces sp. 3022a. 3-Deoxy-D-arabino-heptulosonate 7-Phosphate Synthetase

1971 ◽  
Vol 49 (4) ◽  
pp. 448-455 ◽  
Author(s):  
D. A. Lowe ◽  
D. W. S. Westlake
Keyword(s):  
Enzyme Activity ◽  
Shikimic Acid ◽  
Aromatic Amino Acids ◽  
Product Inhibition ◽  
Enzyme Synthesis ◽  
Synthetase Activity ◽  
Shikimic Acid Pathway ◽  
Sugar Phosphates ◽  
Acid Pathway ◽  
Single Enzyme

The repression and end-product inhibition of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase were studied in a chloramphenicol-producing Streptomycetes. Synthesis of DAHP synthetase was repressed by p-hydroxybenzoate, and enzyme activity was inhibited competitively by sugar phosphates, especially D-ribose 5-phosphate. The presence of chloramphenicol, aromatic amino acids, or shikimic acid pathway intermediates did not repress enzyme synthesis nor inhibit enzyme activity. Chloramphenicol production by growing cultures was not affected by the intermediates or end products of the shikimic acid pathway nor by the repression of DAHP synthetase. Purification of DAHP synthetase activity indicated the presence of a single enzyme protein with a molecular weight of 88 000.

scholarly journals Correspondence on “Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts”

ACS Catalysis ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 3591-3593 ◽  
Author(s):  
Alina N. Sekretaryova ◽  
Mikhail Yu. Vagin ◽  
Anthony P. F. Turner ◽  
Mats Eriksson
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

scholarly journals Reply to Comment on “Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts”

ACS Catalysis ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 3594-3596 ◽  
Author(s):  
Enno Kätelhön ◽  
Lior Sepunaru ◽  
Arkady A. Karyakin ◽  
Richard G. Compton
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

scholarly journals Single-molecule enzymology: What can it tell us?

2003 ◽  
Vol 25 (4) ◽  
pp. 24-27 ◽  
Author(s):  
Clive R. Bagshaw
Keyword(s):  
Enzyme Activity ◽  
Single Molecule ◽  
Enzyme Molecule ◽  
Basic Principles ◽  
Single Enzyme ◽  
Single Molecule Enzymology

In the last decade, several methods have been developed to measure enzyme activity at the level of a single enzyme molecule. This represents a considerable technical feat, but what does it allow us to learn about enzymes? Here, the basic principles are reviewed to show that new forms of heterogeneity in activity may be revealed and evidence gained for rare states that would otherwise be swamped in bulk assays.

scholarly journals Pyridine Nucleosidase in Bull Semen ll. Biochemical Properties

1975 ◽  
Vol 28 (3) ◽  
pp. 273 ◽  
Author(s):  
KH Giles ◽  
KL Macmillan
Keyword(s):  
Enzyme Activity ◽  
Biochemical Properties ◽  
Time Intervals ◽  
Preliminary Heating ◽  
Bull Semen ◽  
Enzyme Denaturation ◽  
Inhibited Enzyme ◽  
Single Enzyme

It is most likely a single enzyme (NAD+ nucleosidase) present in semen from most bulls which hydrolyses the ribosyl pyridinium bond in both NAD and NADP. This conclusion is based on the following results: (i) each of 12 semen samples containing nucleosidase activity hydrolysed NAD at the same rate as NADP (r = 0�99); (ii) other untreated semen samples from different bulls which did not hydrolyse NAD were also inactive against NADP; (iii) enzyme denaturation produced by preliminary heating of semen filtrates for 15 min at varied temperatures or by heating at 55�C for varied time intervals caused similar reductions in the rates of NAD and NADP hydrolysis; and (iv) nicotinamide inhibited enzyme activity to the same degree using either NAD or NADP as the substrate.

scholarly journals Single Enzyme Activity Detected with a Nanoelectronic Sensor

2013 ◽  
Vol 104 (2) ◽  
pp. 518a
Author(s):  
Landon Prisbrey ◽  
Tal Sharf ◽  
Sophie Ripp ◽  
Kerstin Blank ◽  
Ethan D. Minot
Keyword(s):  
Enzyme Activity ◽  
Single Enzyme

scholarly journals Synthesis and single enzyme activity of a clicked lipase–BSA hetero-dimer

2006 ◽  
pp. 2012-2014 ◽  
Author(s):  
Nikos S. Hatzakis ◽  
Hans Engelkamp ◽  
Kelly Velonia ◽  
Johan Hofkens ◽  
Peter C. M. Christianen ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Single Enzyme

scholarly journals Dynamics of Single-Enzyme Activity in a Nanopore Confinement

2018 ◽  
Vol 114 (3) ◽  
pp. 688a
Author(s):  
Yao Lin ◽  
Yilun Ying ◽  
Rui Gao ◽  
Yitao Long
Keyword(s):  
Enzyme Activity ◽  
Nanopore Confinement ◽  
Single Enzyme

scholarly journals Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples

2020 ◽  
Vol 6 (11) ◽  
pp. eaay0888 ◽  
Author(s):  
Shingo Sakamoto ◽  
Toru Komatsu ◽  
Rikiya Watanabe ◽  
Yi Zhang ◽  
Taiki Inoue ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Single Molecule ◽  
Biological Samples ◽  
Protein Profiling ◽  
Single Molecule Level ◽  
Multiple Substrates ◽  
Hydrolyzing Enzymes ◽  
Enzyme Molecules ◽  
Related Proteins ◽  
Single Enzyme

We established an ultrasensitive method for identifying multiple enzymes in biological samples by using a multiplexed microdevice-based single-molecule enzymatic assay. We used a paradigm in which we “count” the number of enzyme molecules by profiling their single enzyme activity characteristics toward multiple substrates. In this proof-of-concept study of the single enzyme activity–based protein profiling (SEAP), we were able to detect the activities of various phosphoric ester–hydrolyzing enzymes such as alkaline phosphatases, tyrosine phosphatases, and ectonucleotide pyrophosphatases in blood samples at the single-molecule level and in a subtype-discriminating manner, demonstrating its potential usefulness for the diagnosis of diseases based on ultrasensitive detection of enzymes.

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