scholarly journals Reply to Comment on “Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts”

ACS Catalysis ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 3594-3596 ◽  
Author(s):  
Enno Kätelhön ◽  
Lior Sepunaru ◽  
Arkady A. Karyakin ◽  
Richard G. Compton
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

scholarly journals Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts

ACS Catalysis ◽  
2016 ◽  
Vol 6 (12) ◽  
pp. 8313-8320 ◽  
Author(s):  
Enno Kätelhön ◽  
Lior Sepunaru ◽  
Arkady A. Karyakin ◽  
Richard G. Compton
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

scholarly journals Correspondence on “Can Nanoimpacts Detect Single-Enzyme Activity? Theoretical Considerations and an Experimental Study of Catalase Impacts”

ACS Catalysis ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 3591-3593 ◽  
Author(s):  
Alina N. Sekretaryova ◽  
Mikhail Yu. Vagin ◽  
Anthony P. F. Turner ◽  
Mats Eriksson
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Theoretical Considerations ◽  
Single Enzyme

Regulation of Chloramphenicol Synthesis in Streptomyces sp. 3022a. 3-Deoxy-D-arabino-heptulosonate 7-Phosphate Synthetase

1971 ◽  
Vol 49 (4) ◽  
pp. 448-455 ◽  
Author(s):  
D. A. Lowe ◽  
D. W. S. Westlake
Keyword(s):  
Enzyme Activity ◽  
Shikimic Acid ◽  
Aromatic Amino Acids ◽  
Product Inhibition ◽  
Enzyme Synthesis ◽  
Synthetase Activity ◽  
Shikimic Acid Pathway ◽  
Sugar Phosphates ◽  
Acid Pathway ◽  
Single Enzyme

The repression and end-product inhibition of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase were studied in a chloramphenicol-producing Streptomycetes. Synthesis of DAHP synthetase was repressed by p-hydroxybenzoate, and enzyme activity was inhibited competitively by sugar phosphates, especially D-ribose 5-phosphate. The presence of chloramphenicol, aromatic amino acids, or shikimic acid pathway intermediates did not repress enzyme synthesis nor inhibit enzyme activity. Chloramphenicol production by growing cultures was not affected by the intermediates or end products of the shikimic acid pathway nor by the repression of DAHP synthetase. Purification of DAHP synthetase activity indicated the presence of a single enzyme protein with a molecular weight of 88 000.

Hydraulics of steeppass fishways

1991 ◽  
Vol 18 (6) ◽  
pp. 1024-1032 ◽  
Author(s):  
N. Rajaratnam ◽  
C. Katopodis
Keyword(s):  
Experimental Study ◽  
Velocity Profile ◽  
Channel Flow ◽  
Flow Rate ◽  
Open Channel ◽  
Maximum Velocity ◽  
Open Channel Flow ◽  
Width Ratio ◽  
Hydraulic Design ◽  
Theoretical Considerations

This paper presents the results of an experimental study on the hydraulics of steeppass fishways. Using theoretical considerations and experimental observations, an expression has been developed that relates the flow rate, slope of the fishway, and depth of flow. It was also found that the characteristic (similarity) velocity profile found earlier, for smaller values of depth to width ratio,y0/b, with the maximum velocity near the bottom, changes to a rather symmetrical profile with the maximum velocity occurring somewhere near the mid-depth for larger values of y0/b. A correlation has also been found for the maximum velocity. This paper also includes some observations on the M-type backwater curves that would appear in the fishway when the tailwater depths exceed uniform flow depths. Key words: fishways, hydraulics, turbulent flow, open-channel flow, hydraulic design.

scholarly journals Single-molecule enzymology: What can it tell us?

2003 ◽  
Vol 25 (4) ◽  
pp. 24-27 ◽  
Author(s):  
Clive R. Bagshaw
Keyword(s):  
Enzyme Activity ◽  
Single Molecule ◽  
Enzyme Molecule ◽  
Basic Principles ◽  
Single Enzyme ◽  
Single Molecule Enzymology

In the last decade, several methods have been developed to measure enzyme activity at the level of a single enzyme molecule. This represents a considerable technical feat, but what does it allow us to learn about enzymes? Here, the basic principles are reviewed to show that new forms of heterogeneity in activity may be revealed and evidence gained for rare states that would otherwise be swamped in bulk assays.

scholarly journals Pyridine Nucleosidase in Bull Semen ll. Biochemical Properties

1975 ◽  
Vol 28 (3) ◽  
pp. 273 ◽  
Author(s):  
KH Giles ◽  
KL Macmillan
Keyword(s):  
Enzyme Activity ◽  
Biochemical Properties ◽  
Time Intervals ◽  
Preliminary Heating ◽  
Bull Semen ◽  
Enzyme Denaturation ◽  
Inhibited Enzyme ◽  
Single Enzyme

It is most likely a single enzyme (NAD+ nucleosidase) present in semen from most bulls which hydrolyses the ribosyl pyridinium bond in both NAD and NADP. This conclusion is based on the following results: (i) each of 12 semen samples containing nucleosidase activity hydrolysed NAD at the same rate as NADP (r = 0�99); (ii) other untreated semen samples from different bulls which did not hydrolyse NAD were also inactive against NADP; (iii) enzyme denaturation produced by preliminary heating of semen filtrates for 15 min at varied temperatures or by heating at 55�C for varied time intervals caused similar reductions in the rates of NAD and NADP hydrolysis; and (iv) nicotinamide inhibited enzyme activity to the same degree using either NAD or NADP as the substrate.

scholarly journals Single Enzyme Activity Detected with a Nanoelectronic Sensor

2013 ◽  
Vol 104 (2) ◽  
pp. 518a
Author(s):  
Landon Prisbrey ◽  
Tal Sharf ◽  
Sophie Ripp ◽  
Kerstin Blank ◽  
Ethan D. Minot
Keyword(s):  
Enzyme Activity ◽  
Single Enzyme

Modeling the Electrostatic Signature of Single Enzyme Activity

2010 ◽  
Vol 114 (9) ◽  
pp. 3330-3333 ◽  
Author(s):  
Landon Prisbrey ◽  
Guenter Schneider ◽  
Ethan Minot
Keyword(s):  
Enzyme Activity ◽  
Single Enzyme

The relationship between enhanced enzyme activity and structural dynamics in ionic liquids: a combined computational and experimental study

2014 ◽  
Vol 16 (7) ◽  
pp. 2944 ◽  
Author(s):  
Ho Shin Kim ◽  
Sung Ho Ha ◽  
Latsavongsakda Sethaphong ◽  
Yoon-Mo Koo ◽  
Yaroslava G. Yingling
Keyword(s):  
Experimental Study ◽  
Enzyme Activity ◽  
Ionic Liquids ◽  
Structural Dynamics ◽  
The Relationship
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