Site-Specific Dynamics in TAT Triplex DNA As Revealed by Time-Domain Fluorescence of 2-Aminopurine

2009 ◽  
Vol 113 (19) ◽  
pp. 6840-6846 ◽  
Author(s):  
T. Ramreddy ◽  
Mamata Kombrabail ◽  
G. Krishnamoorthy ◽  
B. J. Rao
Keyword(s):  
Time Domain ◽  
Triplex Dna ◽  
Site Specific

scholarly journals Toward site-specific, homogeneous and highly stable fluorescent silver nanoclusters fabrication on triplex DNA scaffolds

2012 ◽  
Vol 40 (16) ◽  
pp. e122-e122 ◽  
Author(s):  
Lingyan Feng ◽  
Zhenzhen Huang ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Silver Nanoclusters ◽  
Triplex Dna ◽  
Site Specific ◽  
Dna Scaffolds

scholarly journals Site Specific Response Analysis Kota Padang dari Input motion Conditional Mean Spectrum (CMS) Menggunakan Software NERA

2017 ◽  
Vol 14 (2) ◽  
pp. 28-38
Author(s):  
Merley Misriani
Keyword(s):  
Ground Motion ◽  
Time Domain ◽  
Earthquake Response ◽  
Response Analysis ◽  
Specific Response ◽  
Site Specific ◽  
Conditional Mean ◽  
Conditional Mean Spectrum ◽  
Input Motion ◽  
Earthquake Response Analysis

Dalam perencanaan struktur bangunan tahan gempa, tujuan umum dari analisa struktur dinamis adalah memprediksi respons dari struktur terhadap pengaruh ground motion yang memiliki Spectral Acceleration (Sa) pada periode tertentu berdasarkan tingkat kemungkinan terlampui (Probability of Excedence/PE) 10% atau 2% masa layan bangunan 50 tahun.  Prediksi respons struktur ditentukan dengan memilih ground motion yang cocok dengan beberapa target spektra dan nantinya ground motion tersebut digunakan sebagai input dalam analisis dinamis struktur. Pada paper ini menyajikan analisis perambatan gelombang gempa dari batuan dasar ke lapisan permukaan (Site Specific Response Analysis/ SSRA). Data-data yang diperlukan adalah data stratifikasi tanah dan parameter kecepatan gelombang geser yang didapatkan dari korelasi empiris terhadap data hasil pemboran dan uji N-SPT. Ground motion synthetic batuan dasar yang digunakan untuk perambatan gelombang gempa diperoleh dari hasil penelitian sebelumnya yang mengadopsi pendekatan statistik Conditional Mean Spectrum (CMS) agar ground motion  yang dihasilkan dipermukaan cocok dengan prediksi masalah respon struktur yang sebenarnya. SSRA dilakukan berdasarkan teori perambatan gelombang geser satu dimensi dalam time domain dengan menggunakan program Non-linear Earthquake Response Analysis (NERA). Hasilnya diperoleh percepatan maksimum gempa dipermukaan (peak surface acceleration/ PBA) berbeda-beda di kedua lokasi tergantung pada faktor amplifikasi, karakteristik dan jenis gempa yang terjadi. Direkomendasikan respons spectra desain untuk periode ulang 475 tahun dan 2475 tahun wilayah 4 kelas tanah sedang (SD) untuk kedua lokasi tersebut. Data-data tersebut digunakan sebagai input dalam penentuan beban gempa pada bangunan dalam analisis struktur dinamis.

Motion Feasibility Framework for Remotely Operated Vehicles Based on Dynamic Positioning Capability

2020 ◽  
Vol 143 (1) ◽  
Author(s):  
Juan A. Ramírez-Macías ◽  
Rafael E. Vásquez ◽  
Asgeir J. Sørensen ◽  
Svein Sævik
Keyword(s):  
Steady State ◽  
Time Domain ◽  
Environmental Data ◽  
Specific Information ◽  
Dynamic Positioning ◽  
Remotely Operated Vehicle ◽  
Site Specific ◽  
Numerical Examples ◽  
Work Training ◽  
System Components

Abstract Knowing whether a remotely operated vehicle (ROV) is able to operate at certain foreknown environmental conditions is a question relevant to different actors during the vehicle’s life cycle: during design stages, buying an ROV, planning operations, and performing an operation. This work addresses a framework to assess motion feasibility in ROVs by using the concept of ROV-dynamic positioning capability (ROV-DPCap). Within the proposed framework, the ROV-DPCap number is defined to measure motion capability, and ROV-DPCap plots are used to illustrate results, for quasi-static standard (L2) and site-specific (L2s) conditions, and dynamic standard (L3) and site-specific (L3s) conditions. Data are computed by steady-state or time-domain simulations from the ROV model, depending on the desired analysis. To illustrate the use of the framework, numerical examples for L2 and L2s motion feasibility analyses for NTNU’s ROV Minerva are provided. Motion feasibility can be used to know whether an ROV is appropriately designed for a specific operation and choose the appropriate one for a certain need, for instance, when designing the DP system components or planning an operation from the environmental data and ROV-specific information. As expected, predictions can be improved when more detailed information about the ROV appears; the same framework can be used to provide more detailed answers to motion feasibility-related questions. The results are likely to be straightforwardly understood by people whose work/training is ROV related and can interpret the graphic results for different operation scenarios.

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

Sign in / Sign up

Export Citation Format

Share Document