Mechanism of Site-Specific Psoralen Photoadducts Formation in Triplex DNA Directed by Psoralen-Conjugated Oligonucleotides†

Yueh-Hsin Ping; Tariq M. Rana

doi:10.1021/bi0488707

Mechanism of Site-Specific Psoralen Photoadducts Formation in Triplex DNA Directed by Psoralen-Conjugated Oligonucleotides†

Biochemistry ◽

10.1021/bi0488707 ◽

2005 ◽

Vol 44 (7) ◽

pp. 2501-2509 ◽

Cited By ~ 12

Author(s):

Yueh-Hsin Ping ◽

Tariq M. Rana

Keyword(s):

Triplex Dna ◽

Site Specific

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Site-Specific Dynamics in TAT Triplex DNA As Revealed by Time-Domain Fluorescence of 2-Aminopurine

The Journal of Physical Chemistry B ◽

10.1021/jp901216h ◽

2009 ◽

Vol 113 (19) ◽

pp. 6840-6846 ◽

Cited By ~ 15

Author(s):

T. Ramreddy ◽

Mamata Kombrabail ◽

G. Krishnamoorthy ◽

B. J. Rao

Keyword(s):

Time Domain ◽

Triplex Dna ◽

Site Specific

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Toward site-specific, homogeneous and highly stable fluorescent silver nanoclusters fabrication on triplex DNA scaffolds

Nucleic Acids Research ◽

10.1093/nar/gks387 ◽

2012 ◽

Vol 40 (16) ◽

pp. e122-e122 ◽

Cited By ~ 66

Author(s):

Lingyan Feng ◽

Zhenzhen Huang ◽

Jinsong Ren ◽

Xiaogang Qu

Keyword(s):

Silver Nanoclusters ◽

Triplex Dna ◽

Site Specific ◽

Dna Scaffolds

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Large-cluster and combined fluorescent and gold immunoprobes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166920 ◽

1996 ◽

Vol 54 ◽

pp. 892-893

Author(s):

Richard D. Powell ◽

James F. Hainfeld ◽

Carol M. R. Halsey ◽

David L. Spector ◽

Shelley Kaurin ◽

...

Keyword(s):

Metal Cluster ◽

Sulfonyl Chloride ◽

Gel Filtration ◽

Cross Linking ◽

Site Specific ◽

Fab Fragments ◽

Cluster Label ◽

Covalently Linked ◽

Texas Red ◽

Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

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The challenge of detecting modifications on proteins

Essays in Biochemistry ◽

10.1042/ebc20190055 ◽

2020 ◽

Vol 64 (1) ◽

pp. 135-153 ◽

Cited By ~ 1

Author(s):

Lauren Elizabeth Smith ◽

Adelina Rogowska-Wrzesinska

Keyword(s):

Mass Spectrometry ◽

Protein Function ◽

Chemical Properties ◽

Lysine Acetylation ◽

Mass Determination ◽

Post Translational Modifications ◽

Site Specific ◽

The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

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