Design and Preparation of a Novel Temperature-Responsive Ionic Gel. 3. Valence Selective Control of Transport Modes of Ions in Response to Temperature

2008 ◽  
Vol 112 (21) ◽  
pp. 6585-6593 ◽  
Author(s):  
Mitsuru Higa ◽  
Tomoko Yamakawa
e-Polymers ◽  
2007 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiao Xin-Cai

AbstractPoly(N-isopropylacrylamide) hydrogels have been successfully modified by concentrated sulfuric acid for the first time. The modified hydrogels displayed faster, larger magnitude and hydration/dehydration dynamic response to temperature cycling without increasing the lower critical solution temperature (LCST). These contributions were attributed to sulphate ester groups resulting from terminal hydroxyl groups of poly(N-isopropylacrylamide). These results may lead to technological application for temperature-responsive thin film and microgel particles with higher surface-to-volume ratio.


2015 ◽  
Vol 3 (48) ◽  
pp. 9285-9294 ◽  
Author(s):  
Alissa J. Hackett ◽  
Jenny Malmström ◽  
Paul J. Molino ◽  
Julien E. Gautrot ◽  
Hongrui Zhang ◽  
...  

Salt- and temperature-responsive P(PEGMMA)-based brushes were grafted from conducting polymer films to produce electroactive surfaces with tailored switching behaviour.


2019 ◽  
Author(s):  
Lauren Rodriguez ◽  
Mark Voorhies ◽  
Sarah Gilmore ◽  
Sinem Beyhan ◽  
Anthony Myint ◽  
...  

ABSTRACTPhenotypic switching between two opposing cellular states is a fundamental aspect of biology, and fungi provide facile systems to analyze the interactions between regulons that control this type of switch. A long-standing mystery in fungal pathogens of humans is how thermally dimorphic fungi switch their developmental form in response to temperature. These fungi, including the subject of this study,Histoplasma capsulatum, are temperature-responsive organisms that utilize unknown regulatory pathways to couple their cell shape and associated attributes to the temperature of their environment.H. capsulatumgrows as a multicellular hypha in the soil that switches to a pathogenic yeast form in response to the temperature of a mammalian host. These states can be triggered in the laboratory simply by growing the fungus either at room temperature (where it grows as hyphae) or at 37°C (where it grows as yeast). Prior worked revealed that 15-20% of transcripts are differentially expressed in response to temperature, but it is unclear which transcripts are linked to specific phenotypic changes such as cell morphology or virulence. To elucidate temperature-responsive regulons, we previously identified four transcription factors (Ryp1-4) that are required for yeast-phase growth at 37°C; in eachrypmutant, the fungus grows constitutively as hyphae regardless of temperature and the cells fail to express genes that are normally induced in response to growth at 37°C. Here we perform the first genetic screen to identify genes required for hyphal growth ofH. capsulatumat room temperature and find that disruption of the signaling mucinMSB2results in a yeast-locked phenotype. RNAseq experiments reveal thatMSB2is not required for the majority of gene expression changes that occur when cells are shifted to room temperature. However, a small subset of temperature-responsive genes is dependent onMSB2for its expression, thereby implicating these genes in the process of filamentation. Disruption or knockdown of an Msb2-dependent MAP kinase (HOG2) and an APSES transcription factor (STU1) prevents hyphal growth at room temperature, validating that the Msb2 regulon contains genes that control filamentation. Notably, the Msb2 regulon shows conserved hyphal-specific expression in other dimorphic fungi, suggesting that this work defines a small set of genes that are likely to be conserved regulators and effectors of filamentation in multiple fungi. In contrast, a few yeast-specific transcripts, including virulence factors that are normally expressed only at 37°C, are inappropriately expressed at room temperature in themsb2mutant, suggesting that expression of these genes is coupled to growth in the yeast form rather than to temperature. Finally, we find that the yeast-promoting transcription factor Ryp3 associates with theMSB2promoter and inhibitsMSB2transcript expression at 37°C, whereas Msb2 inhibits accumulation of Ryp transcripts and proteins at room temperature. These findings indicate that the Ryp and Msb2 circuits antagonize each other in a temperature-dependent manner, thereby allowing temperature to govern cell shape and gene expression in this ubiquitous fungal pathogen of humans.


Author(s):  
Lukas Kronenberg ◽  
Steven Yates ◽  
Martin P Boer ◽  
Norbert Kirchgessner ◽  
Achim Walter ◽  
...  

Abstract In wheat, temperature affects the timing and intensity of stem elongation. Genetic variation for this process is therefore important for adaptation. This study investigates the genetic response to temperature fluctuations during stem elongation and its relationship to phenology and height. Canopy height of 315 wheat genotypes (GABI wheat panel) was scanned twice weekly in the field phenotyping platform (FIP) of ETH Zurich using a LIDAR. Temperature response was modelled using linear regressions between stem elongation and mean temperature in each measurement interval. This led to a temperature-responsive (slope) and a temperature-irresponsive (intercept) component. The temperature response was highly heritable (H2=0.81) and positively related to a later start and end of stem elongation as well as final height. Genome-wide association mapping revealed three temperature-responsive and four temperature-irresponsive quantitative trait loci (QTLs). Furthermore, putative candidate genes for temperature-responsive QTLs were frequently related to the flowering pathway in Arabidopsis thaliana, whereas temperature-irresponsive QTLs corresponded to growth and reduced height genes. In combination with Rht and Ppd alleles, these loci, together with the loci for the timing of stem elongation, accounted for 71% of the variability in height. This demonstrates how high-throughput field phenotyping combined with environmental covariates can contribute to a smarter selection of climate-resilient crops.


2019 ◽  
Author(s):  
Lukas Kronenberg ◽  
Steven Yates ◽  
Martin P. Boer ◽  
Norbert Kirchgessner ◽  
Achim Walter ◽  
...  

AbstractIn wheat, temperature affects the timing and intensity of stem elongation (SE). Genetic variation for this process is therefore important for adaptation. This study investigates the genetic response to temperature fluctuations during SE and its relationship to phenology and height. Canopy height of 315 wheat genotypes (GABI wheat panel) was scanned twice weekly in the field phenotyping platform (FIP) of ETH Zurich using a LIDAR. Temperature response was modelled using linear regressions between SE and mean temperature in each measurement interval. This led to a temperature–responsive (slope) and a temperature-irresponsive (intercept) component.The temperature response was highly heritable (H2 = 0.81) and positively related to a later start and end of SE as well as final height. Genome-wide association mapping revealed three temperature-responsive and four temperature-irresponsive quantitative trait loci (QTL). Furthermore, putative candidate genes for temperature-response QTL were frequently related to the flowering pathway in A. thaliana, whereas temperature-irresponsive QTLs corresponded with growth and reduced height genes. In combination with Rht and Ppd alleles, these loci, together with the loci for the timing of SE accounted for 71% of the variability in height.This demonstrates how high-throughput field phenotyping combined with environmental covariates can contribute to a smarter selection of climate-resilient crops.HighlightWe measured ambient temperature response of stem elongation in wheat grown under field conditions. The results indicate that temperature response is highly heritable and linked to the flowering pathway.


2017 ◽  
Vol 5 (24) ◽  
pp. 12474-12482 ◽  
Author(s):  
Parvin Karimineghlani ◽  
Emily Emmons ◽  
Micah J. Green ◽  
Patrick Shamberger ◽  
Svetlana A. Sukhishvili

A temperature-responsive PVA gel is achieved that reversibly holds fluid lithium nitrate trihydrate and releases it in response to temperature for easy gelling in-place and later removal from heat-exchange modules.


2016 ◽  
Author(s):  
Arjen Gebraad ◽  
Teuvo Hentunen ◽  
Tiina Laitala-Leinonen

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