Structural Properties of the Water/Membrane Interface of a Bilayer Built of theE. coliLipid A

2015 ◽  
Vol 119 (18) ◽  
pp. 5846-5856 ◽  
Author(s):  
Krzysztof Murzyn ◽  
Marta Pasenkiewicz-Gierula
Author(s):  
Jeffry A. Reidler ◽  
John P. Robinson

We have prepared two-dimensional (2D) crystals of tetanus toxin using procedures developed by Uzgiris and Kornberg for the directed production of 2D crystals of monoclonal antibodies at an antigen-phospholipid monolayer interface. The tetanus toxin crystals were formed using a small mole fraction of the natural receptor, GT1, incorporated into phosphatidyl choline monolayers. The crystals formed at low concentration overnight. Two dimensional crystals of this type are particularly useful for structure determination using electron microscopy and computer image refinement. Three dimensional (3D) structural information can be derived from these crystals by computer reconstruction of photographs of toxin crystals taken at different tilt angles. Such 3D reconstructions may help elucidate the mechanism of entry of the enzymatic subunit of toxins into cells, particularly since these crystals form directly on a membrane interface at similar concentrations of ganglioside GT1 to the natural cellular receptors.


1996 ◽  
Vol 1 (3) ◽  
pp. 200-205 ◽  
Author(s):  
Carlo Umiltà ◽  
Francesca Simion ◽  
Eloisa Valenza

Four experiments were aimed at elucidating some aspects of the preference for facelike patterns in newborns. Experiment 1 showed a preference for a stimulus whose components were located in the correct arrangement for a human face. Experiment 2 showed a preference for stimuli that had optimal sensory properties for the newborn visual system. Experiment 3 showed that babies directed their attention to a facelike pattern even when it was presented simultaneously with a non-facelike stimulus with optimal sensory properties. Experiment 4 showed the preference for facelike patterns in the temporal hemifield but not in the nasal hemifield. It was concluded that newborns' preference for facelike patterns reflects the activity of a subcortical system which is sensitive to the structural properties of the stimulus.


1998 ◽  
Vol 08 (PR2) ◽  
pp. Pr2-47-Pr2-50
Author(s):  
O. Crisan ◽  
J. M. Le Breton ◽  
F. Machizaud ◽  
A. Jianu ◽  
J. Teillet ◽  
...  

2000 ◽  
Vol 10 (PR7) ◽  
pp. Pr7-95-Pr7-98 ◽  
Author(s):  
D. Morineau ◽  
F. Casas ◽  
C. Alba-Simionesco ◽  
A. Grosman ◽  
M.-C. Bellissent-Funel ◽  
...  

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