Structural Properties of gp41 Fusion Peptide at a Model Membrane Interface

2013 ◽  
Vol 117 (49) ◽  
pp. 15527-15535 ◽  
Author(s):  
V. Volkov ◽  
M. Bonn
Keyword(s):  
Structural Properties ◽  
Fusion Peptide ◽  
Model Membrane ◽  
Membrane Interface

Destabilization of a model membrane by a predicted fusion peptide of fertilin α

1998 ◽  
Vol 95 (2) ◽  
pp. 467-473 ◽  
Author(s):  
A. Schanck ◽  
R. Brasseur ◽  
J. Peuvot
Keyword(s):  
Fusion Peptide ◽  
Model Membrane

Structural Properties of the Water/Membrane Interface of a Bilayer Built of theE. coliLipid A

2015 ◽  
Vol 119 (18) ◽  
pp. 5846-5856 ◽  
Author(s):  
Krzysztof Murzyn ◽  
Marta Pasenkiewicz-Gierula
Keyword(s):  
Structural Properties ◽  
Membrane Interface

Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface

2017 ◽  
Vol 1861 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Remigiusz Worch ◽  
Joanna Krupa ◽  
Alicja Filipek ◽  
Anna Szymaniec ◽  
Piotr Setny
Keyword(s):  
Fusion Peptide ◽  
Membrane Interface

scholarly journals Structural Properties of Membrane Inserted Fusion Peptide from Influenza Virus Analysed by Molecular Simulation

2014 ◽  
Vol 106 (2) ◽  
pp. 53a
Author(s):  
Diana Lousa ◽  
Bruno L. Victor ◽  
Carlos Fernandez ◽  
Cláudio M. Soares
Keyword(s):  
Influenza Virus ◽  
Structural Properties ◽  
Molecular Simulation ◽  
Fusion Peptide

scholarly journals Membrane Interface-Interacting Sequences within the Ectodomain of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein: Putative Role during Viral Fusion

2000 ◽  
Vol 74 (17) ◽  
pp. 8038-8047 ◽  
Author(s):  
Tatiana Suárez ◽  
William R. Gallaher ◽  
Aitziber Agirre ◽  
Félix M. Goñi ◽  
José L. Nieva
Keyword(s):  
Human Immunodeficiency Virus ◽  
Membrane Fusion ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Fusion Peptide ◽  
Viral Fusion ◽  
Membrane Interface ◽  
Immunodeficiency Virus ◽  
Transmembrane Anchor

ABSTRACT We have identified a region within the ectodomain of the fusogenic human immunodeficiency virus type 1 (HIV-1) gp41, different from the fusion peptide, that interacts strongly with membranes. This conserved sequence, which immediately precedes the transmembrane anchor, is not highly hydrophobic according to the Kyte-Doolittle hydropathy prediction algorithm, yet it shows a high tendency to partition into the membrane interface, as revealed by the Wimley-White interfacial hydrophobicity scale. We have investigated here the membrane effects induced by NH2-DKWASLWNWFNITNWLWYIK-CONH2(HIVc), the membrane interface-partitioning region at the C terminus of the gp41 ectodomain, in comparison to those caused by NH2-AVGIGALFLGFLGAAGSTMGARS-CONH2(HIVn), the fusion peptide at the N terminus of the subunit. Both HIVc and HIVn were seen to induce membrane fusion and permeabilization, although lower doses of HIVc were required for comparable effects to be detected. Experiments in which equimolar mixtures of HIVc and HIVn were used indicated that both peptides may act in a cooperative way. Peptide-membrane and peptide-peptide interactions underlying those effects were further confirmed by analyzing the changes in fluorescence of peptide Trp residues. Replacement of the first three Trp residues by Ala, known to render a defective gp41 phenotype unable to mediate both cell-cell fusion and virus entry, also abrogated the HIVc ability to induce membrane fusion or form complexes with HIVn but not its ability to associate with vesicles. Hydropathy analysis indicated that the presence of two membrane-partitioning stretches separated by a collapsible intervening sequence is a common structural motif among other viral envelope proteins. Moreover, sequences with membrane surface-residing residues preceding the transmembrane anchor appeared to be a common feature in viral fusion proteins of several virus families. According to our experimental results, such a feature might be related to their fusogenic function.

scholarly journals Probing Site-Specific Structural Information of Peptides at Model Membrane Interface In Situ

2015 ◽  
Vol 137 (32) ◽  
pp. 10190-10198 ◽  
Author(s):  
Bei Ding ◽  
Afra Panahi ◽  
Jia-Jung Ho ◽  
Jennifer E. Laaser ◽  
Charles L. Brooks ◽  
...  
Keyword(s):  
Structural Information ◽  
Model Membrane ◽  
Membrane Interface ◽  
Site Specific

NMR Studies of a13C,15N-Labeled GM4-Lactam Glycolipid at an Oriented Model-Membrane Interface

1996 ◽  
Vol 118 (17) ◽  
pp. 4001-4008 ◽  
Author(s):  
Brian A. Salvatore ◽  
Ranajeet Ghose ◽  
James H. Prestegard
Keyword(s):  
Model Membrane ◽  
Membrane Interface ◽  
Nmr Studies
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