Evaluation of Aqueous and Nonaqueous Binary Solvent Mixtures as Mobile Phase Alternatives to Water–Acetonitrile Mixtures for Hydrophilic Interaction Liquid Chromatography by Molecular Dynamics Simulations

2014 ◽  
Vol 119 (1) ◽  
pp. 512-523 ◽  
Author(s):  
Sergey M. Melnikov ◽  
Alexandra Höltzel ◽  
Andreas Seidel-Morgenstern ◽  
Ulrich Tallarek
Keyword(s):  
Molecular Dynamics ◽  
Liquid Chromatography ◽  
Molecular Dynamics Simulations ◽  
Mobile Phase ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Binary Solvent ◽  
Solvent Mixtures ◽  
Hydrophilic Interaction ◽  
Binary Solvent Mixtures ◽  
Dynamics Simulations

Studying Binary Solvent Mixtures as Mobile Phase for Thermal Gradient Interactive Chromatography (TGIC) of Poly(ethylene-stat-1-octene)

2014 ◽  
Vol 53 (39) ◽  
pp. 15183-15191 ◽  
Author(s):  
Dibyaranjan Mekap ◽  
Tibor Macko ◽  
Robert Brüll ◽  
Rongjuan Cong ◽  
A. Willem deGroot ◽  
...  
Keyword(s):  
Thermal Gradient ◽  
Mobile Phase ◽  
Binary Solvent ◽  
Solvent Mixtures ◽  
Binary Solvent Mixtures ◽  
Poly Ethylene

scholarly journals Fast HPLC Method Using Ion-Pair and Hydrophilic Interaction Liquid Chromatography for Determination of Phenylephrine in Pharmaceutical Formulations

2010 ◽  
Vol 93 (5) ◽  
pp. 1436-1442 ◽  
Author(s):  
Michal Douòa ◽  
Petr Gibala
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Flow Rate ◽  
Mobile Phase ◽  
Hplc Method ◽  
Ion Pair ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Injection Volume ◽  
Ion Pair Chromatography

Abstract A rapid procedure based on a direct extraction and HPLC determination with fluorescence detection of phenylephrine in pharmaceutical sachets that include a large excess of paracetamol (65 + 1, w/w), ascorbic acid (5 + 1, w/w), and other excipients (aspartame and sucrose) was developed and validated. The final optimized chromatographic method for ion-pair chromatography used an XTerra RP18 column, 3 µm particle size, 50 ⨯ 3.0 mm id. The mobile phase consisted of a mixture of acetonitrile and buffer (10 mM sodium octane-1-sulfonate, adjusted with H3PO4 to pH 2.2; 200 + 800, v/v), with a constant flow rate of 0.3 mL/min. The separation was carried out at 30C, and the injection volume was 3 µL. Fluorescence detection was performed at excitation and emission wavelengths of 275 and 310 nm, respectively. The mobile phase parameters, such as the organic solvent fraction (acetonitrile) in mobile phase as an organic modifier, the concentration of sodium octane-1-sulfonate as a counter-ion, temperature, and pH of mobile phase, were studied. As an alternative to ion-pair chromatography, hydrophilic interaction liquid chromatography (HILIC) was investigated using a Luna HILIC column, 3 m, 100 ⨯ 4.6 mm id. The mobile phase consisted of acetonitrile and buffer (5 M potassium dihydrogen phosphate, adjusted with H3PO4 to pH 2.5; 750 + 250, v/v) at a flow rate of 0.8 mL/min. The separation was carried out at 25°C, and the injection volume was 5 µL. The proposed method has an advantage of a very simple sample pretreatment, and is much faster than the currently utilized HPLC methods using gradient elution and UV detection. Commercial samples of sachets were successfully analyzed by the proposed HPLC method.

Molecular Dynamics Study of Polysaccharides in Binary Solvent Mixtures of an Ionic Liquid and Water

2011 ◽  
Vol 115 (34) ◽  
pp. 10251-10258 ◽  
Author(s):  
Hanbin Liu ◽  
Kenneth L. Sale ◽  
Blake A. Simmons ◽  
Seema Singh
Keyword(s):  
Molecular Dynamics ◽  
Ionic Liquid ◽  
Binary Solvent ◽  
Solvent Mixtures ◽  
Binary Solvent Mixtures
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