scholarly journals A Molecular Dynamics Study on the Retention Mechanism in Hydrophilic Interaction Liquid Chromatography

2014 ◽  
Vol 86 (9) ◽  
pp. 1631-1632
Author(s):  
S. M. Melnikov ◽  
A. Hoeltzel ◽  
A. Seidel-Morgenstern ◽  
U. Tallarek
Keyword(s):  
Molecular Dynamics ◽  
Liquid Chromatography ◽  
Retention Mechanism ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Hydrophilic Interaction Liquid Chromatography and Per Aqueous Liquid Chromatography in Fungicides Analysis

2012 ◽  
Vol 95 (5) ◽  
pp. 1362-1370 ◽  
Author(s):  
Sylwia Noga ◽  
Attila Felinger ◽  
Bogusław Buszewski
Keyword(s):  
Liquid Chromatography ◽  
Organic Solvent ◽  
Mobile Phase ◽  
Retention Mechanism ◽  
Operating Conditions ◽  
Biologically Active ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Active Compounds ◽  
Retention Mechanisms

Abstract The goal of the study was to investigate the retention mechanism of selected fungicides in hydrophilic interaction liquid chromatography (HILIC) and per aqueous liquid chromatography (PALC). Chromatographic measurements were made on four physicochemically diversified HILIC columns, which were evaluated for the analysis of nine biologically active compounds, such as strobilurins and triazoles. The effects of the operating conditions on separations were investigated, including the concentration of the organic solvent in the aqueous-organic (acetonitrile) mobile phase. The results were compared, and it was shown that two different retention mechanisms dominate in PALC at low acetonitrile concentrations and in HILIC at high acetonitrile concentrations.

scholarly journals Insights into the Mechanism of Separation of Bisphosphonates by Zwitterionic Hydrophilic Interaction Liquid Chromatography: Application to the Quantitation of Risedronate in Pharmaceuticals

Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 6 ◽  
Author(s):  
Irene Panderi ◽  
Eugenia Taxiarchi ◽  
Constantinos Pistos ◽  
Eleni Kalogria ◽  
Ariadni Vonaparti
Keyword(s):  
Liquid Chromatography ◽  
Polar Compounds ◽  
Reversed Phase ◽  
Retention Mechanism ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Percentage Error ◽  
Hydrophilic Interaction ◽  
Reversed Phase Liquid Chromatography ◽  
Short Run ◽  
Phase Liquid

Bisphosphonates are used to treat various skeletal disorders, as they modulate bone metabolism by inhibition of the osteoclast-mediated bone resorption. These compounds are both polar and ionic, and therefore, by using reversed phase liquid chromatography are eluted rapidly. Hydrophilic interaction liquid chromatography (HILIC) is an advantageous technique for the separation and analysis of polar molecules. As the elution order in HILIC is reversed to reversed phase liquid chromatography, a reasonable retention and selectivity for polar compounds is expected. In this work the retention mechanism of three bisphosponates, namely risedronate, tiludronate and zoledronate, was investigated under zwitterionic HILIC conditions. The key factors influencing the retention of the analytes on a zwitterionic ZIC®-pHILIC column (150.0 × 2.1 mm i.d., 200 Å, 3.5 μm) have been systematically investigated. It was found that apart from partition, electrostatic repulsions play an important role in the retention of bisphosphonates. Peak tailing of risedronate and zoledronate was improved by the addition of sodium pyrophosphate in the mobile phase. A zwitterionic hydrophilic interaction liquid chromatography-photodiode array (HILIC-PDA) method was further optimized and fully validated to quantitate risedronate in commercial film-coated tablets. The calibration curves for risedronate showed good linearity (r > 0.9991) within the calibration range tested. The intra- and inter-day coefficient of variation (CV) values was less than 0.6%, while the relative percentage error (%Er) was less than −2.3%. Accelerated stability studies of risedronate conducted under several degradation conditions including hydrolysis, oxidation and heat demonstrated the selectivity of the procedure. A short-run analysis of not more than 6 min allowed the analysis of large samples per day. The applicability of the method for the quantitation of risedronate was demonstrated via the analysis of commercial tablets containing this compound.

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