DNA Assisted Self-Assembly of PAMAM Dendrimers

2014 ◽  
Vol 118 (40) ◽  
pp. 11805-11815 ◽  
Author(s):  
Taraknath Mandal ◽  
Mattaparthi Venkata Satish Kumar ◽  
Prabal K. Maiti
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers

DNA Condensation by PAMAM Dendrimers:  Self-Assembly Characteristics and Effect on Transcription†

Biochemistry ◽  
2008 ◽  
Vol 47 (6) ◽  
pp. 1732-1740 ◽  
Author(s):  
Kristina Fant ◽  
Elin K. Esbjörner ◽  
Per Lincoln ◽  
Bengt Nordén
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers ◽  
Dna Condensation

Dendrimer Mediated ‘Bricks and Mortar’ Self-Assembly of Nanoparticles

2002 ◽  
Vol 739 ◽  
Author(s):  
Benjamin L. Frankamp ◽  
Andrew K. Boal ◽  
Vincent M. Rotello
Keyword(s):  
Magnetic Properties ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Self Assembly ◽  
Quantum Interference ◽  
Pamam Dendrimers ◽  
Interparticle Spacing ◽  
Blocking Temperature ◽  
Oxide Nanoparticles ◽  
Particle Spacing

ABSTRACTControl of particle-particle spacing is a key determinant of optical, electronic, and magnetic properties of nanocomposite materials. We have used poly(amidoamine) (PAMAM) dendrimers to assemble carboxylic acid-functionalized mixed monolayer protected clusters (MMPCs) through acid/base chemistry between particle and polymer. IR spectroscopy and selective dendrimer staining, observed by Transmission Electron Microscopy (TEM), establish that the PAMAM dendrimers are the mortar in the assembly and act to space the MMPCs in the resulting aggregates. Small angle X-ray scattering (SAXS) was then used to establish average interparti cle distances; five generations of PAMAM dendrimer (0, 1, 2, 4, 6) were investigated and monotonic increase in interparticle spacing from 4.1 nm to 6.1 nm was observed.Initial studies involving the application of this methodology to control the magnetic properties of 3-iron oxide nanoparticles have been completed. γ-Iron oxide nanoparticles (6.5 nm in diameter) have been assembled with PAMAM dendrimers generations 2.5, 4.5, and 6.5. The resulting aggregates were characterized with SAXS and magnetization obtained on a super conducting quantum interference devise (SQUID). An observed correlation between the blocking temperature (TB) and the average interparticle spacing suggests that our methodology could be used to tailor the magnetic profile of the nanoparticles.

Solvent-controlled self-assembly of amphiphilic cholic acid-modified PAMAM dendrimers

2011 ◽  
Vol 65 (2) ◽  
pp. 293-295 ◽  
Author(s):  
Kun Zhang ◽  
Ao Yu ◽  
Dawei Wang ◽  
Wenming Yang ◽  
Jinna Li ◽  
...  
Keyword(s):  
Cholic Acid ◽  
Self Assembly ◽  
Pamam Dendrimers

Staged self-assembly of PAMAM dendrimers into macroscopic aggregates with a microribbon structure similar to that of amelogenin

Soft Matter ◽  
2013 ◽  
Vol 9 (31) ◽  
pp. 7553 ◽  
Author(s):  
Jiaojiao Yang ◽  
Shuqin Cao ◽  
Jiahui Li ◽  
Jianyu Xin ◽  
Xingyu Chen ◽  
...  
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers

scholarly journals Controlling the number of dendrimers in dendrimicelle nanoconjugates from 1 to more than 100

Soft Matter ◽  
2014 ◽  
Vol 10 (37) ◽  
pp. 7337-7345 ◽  
Author(s):  
Junyou Wang ◽  
Ilja K. Voets ◽  
Remco Fokkink ◽  
Jasper van der Gucht ◽  
Aldrik H. Velders
Keyword(s):  
Self Assembly ◽  
Diblock Copolymers ◽  
Pamam Dendrimers

The study presents a facile strategy to controllably build up dendrimicelles by self-assembly of anionic PAMAM dendrimers with cationic–neutral diblock copolymers.

Assessment of protective effect of polycation on plasmid DNA attached on the metal surface by SPR

e-Polymers ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Junbo Bao ◽  
Cunxian Song
Keyword(s):  
Molecular Weight ◽  
Protective Effect ◽  
Metal Surface ◽  
Plasmid Dna ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Pamam Dendrimers ◽  
X Ray ◽  
Metal Implants

AbstractSurface modification of the metal is a critical procedure for gene therapy on the metal implants, such as stent and artificial joint. Polycations can condense with DNA by self-assembly, form polyplexes spontaneously as a result of electrostatic interactions and protect DNA from degradation by DNase. The ability of chitosan, polyethyleneimine (PEI), and poly(amidoamine) (PAMAM) dendrimers to condense plasmid DNA(pDNA) was determined by electrophoresis, X-ray photoelectron spectroscopy (XPS) and surface plasmon resonance (SPR). Polycation’s attachment of pDNA to the metal surface was confirmed by XPS which showed no phosphorus peaks on the interface showing the protective effect on pDNA from DNase degradation. Polycations with higher molecular weight or hyperbranched or dendrimer structures can fulfill many of the requirements for effective gene protection from DNase degradation. Chitosan with higher molecular weight (>= 200 kDa) has superior efficiency to protect pDNA against DNase degradation on the surface of the gold chip. Hyperbranched PEIs and PAMAM dendrimers, even with lower molecular weight (for example, 20kDa for PEI, 50kDa for G5) can protect pDNA against DNase degradation. The results of this study present a platform for further optimization studies of polycation-based gene delivery systems.

scholarly journals Fluorescence Spectroscopy of Porphyrins and Phthalocyanines: Some Insights into Supramolecular Self-Assembly, Microencapsulation, and Imaging Microscopy

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4264
Author(s):  
Raquel Teixeira ◽  
Vanda Vaz Serra ◽  
David Botequim ◽  
Pedro M. R. Paulo ◽  
Suzana M. Andrade ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Steady State ◽  
Bovine Serum Albumin ◽  
Self Assembly ◽  
Spectroscopic Studies ◽  
Pamam Dendrimers ◽  
Dynamic State ◽  
Fluorescence Studies ◽  
J Aggregates ◽  
Molecular Complementarity

The molecular interactions of anionic tetrasulfonate phenyl porphyrin (TPPS) with poly(amido amine) (PAMAM) dendrimers of generation 2.0 and 4.0 (G2 and G4, respectively) forming H- or J-aggregates, as well as with human and bovine serum albumin proteins (HSA and BSA), were reviewed in the context of self-assembly molecular complementarity. The spectroscopic studies were extended to the association of aluminum phthtalocyanine (AlPCS4) detected with a PAMAM G4 dendrimer with fluorescence studies in both steady state and dynamic state, as well as due to the fluorescence quenching associated to electron-transfer with a distribution of lifetimes. The functionalization of TPPS with peripheral substituents enables the assignment of spontaneous pH-induced aggregates with different and well-defined morphologies. Other work reported in the literature, in particular with soft self-assembly materials, fall in the same area with particular interest for the environment. The microencapsulation of TPPS studies into polyelectrolyte capsules was developed quite recently and aroused much interest, which is well supported and complemented by the extensive data reported on the Imaging Microscopy section of the Luminescence of Porphyrins and Phthalocyanines included in the present review.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

The Nature of Rapidly Extracted Phycocyanin from the Blue-Green Alga Plectonema Boryanum

1971 ◽  
Vol 29 ◽  
pp. 366-367
Author(s):  
M. Kessel ◽  
R. MacColl
Keyword(s):  
Self Assembly ◽  
Analytical Ultracentrifugation ◽  
Uranyl Acetate ◽  
The Self ◽  
Major Protein ◽  
Subunit Structure ◽  
Blue Green Alga ◽  
Thin Sections ◽  
Blue Green Algae ◽  
Cacodylate Buffer

The major protein of the blue-green algae is the biliprotein, C-phycocyanin (Amax = 620 nm), which is presumed to exist in the cell in the form of distinct aggregates called phycobilisomes. The self-assembly of C-phycocyanin from monomer to hexamer has been extensively studied, but the proposed next step in the assembly of a phycobilisome, the formation of 19s subunits, is completely unknown. We have used electron microscopy and analytical ultracentrifugation in combination with a method for rapid and gentle extraction of phycocyanin to study its subunit structure and assembly.To establish the existence of phycobilisomes, cells of P. boryanum in the log phase of growth, growing at a light intensity of 200 foot candles, were fixed in 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.0, for 3 hours at 4°C. The cells were post-fixed in 1% OsO4 in the same buffer overnight. Material was stained for 1 hour in uranyl acetate (1%), dehydrated and embedded in araldite and examined in thin sections.

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