DNA Condensation by PAMAM Dendrimers:  Self-Assembly Characteristics and Effect on Transcription†

Biochemistry ◽  
2008 ◽  
Vol 47 (6) ◽  
pp. 1732-1740 ◽  
Author(s):  
Kristina Fant ◽  
Elin K. Esbjörner ◽  
Per Lincoln ◽  
Bengt Nordén
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers ◽  
Dna Condensation

Dendrimer Mediated ‘Bricks and Mortar’ Self-Assembly of Nanoparticles

2002 ◽  
Vol 739 ◽  
Author(s):  
Benjamin L. Frankamp ◽  
Andrew K. Boal ◽  
Vincent M. Rotello
Keyword(s):  
Magnetic Properties ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Self Assembly ◽  
Quantum Interference ◽  
Pamam Dendrimers ◽  
Interparticle Spacing ◽  
Blocking Temperature ◽  
Oxide Nanoparticles ◽  
Particle Spacing

ABSTRACTControl of particle-particle spacing is a key determinant of optical, electronic, and magnetic properties of nanocomposite materials. We have used poly(amidoamine) (PAMAM) dendrimers to assemble carboxylic acid-functionalized mixed monolayer protected clusters (MMPCs) through acid/base chemistry between particle and polymer. IR spectroscopy and selective dendrimer staining, observed by Transmission Electron Microscopy (TEM), establish that the PAMAM dendrimers are the mortar in the assembly and act to space the MMPCs in the resulting aggregates. Small angle X-ray scattering (SAXS) was then used to establish average interparti cle distances; five generations of PAMAM dendrimer (0, 1, 2, 4, 6) were investigated and monotonic increase in interparticle spacing from 4.1 nm to 6.1 nm was observed.Initial studies involving the application of this methodology to control the magnetic properties of 3-iron oxide nanoparticles have been completed. γ-Iron oxide nanoparticles (6.5 nm in diameter) have been assembled with PAMAM dendrimers generations 2.5, 4.5, and 6.5. The resulting aggregates were characterized with SAXS and magnetization obtained on a super conducting quantum interference devise (SQUID). An observed correlation between the blocking temperature (TB) and the average interparticle spacing suggests that our methodology could be used to tailor the magnetic profile of the nanoparticles.

Solvent-controlled self-assembly of amphiphilic cholic acid-modified PAMAM dendrimers

2011 ◽  
Vol 65 (2) ◽  
pp. 293-295 ◽  
Author(s):  
Kun Zhang ◽  
Ao Yu ◽  
Dawei Wang ◽  
Wenming Yang ◽  
Jinna Li ◽  
...  
Keyword(s):  
Cholic Acid ◽  
Self Assembly ◽  
Pamam Dendrimers

Amphiphilic dendrons with a pyrene functional group at the focal point: synthesis, self-assembly and generation-dependent DNA condensation

2017 ◽  
Vol 8 (33) ◽  
pp. 4798-4804 ◽  
Author(s):  
Yi Han ◽  
Bo Zhu ◽  
Ying Chen ◽  
Zhishan Bo ◽  
Yulan Chen
Keyword(s):  
Fluorescent Probe ◽  
Self Assembly ◽  
Functional Group ◽  
Focal Point ◽  
Dna Condensation ◽  
Dual Functional

Dendritic amphiphiles with a dual-functional pyrene as a fluorescent probe and hydrophobe at the focal point exhibited generation-dependent self-assembly and DNA condensation.

Staged self-assembly of PAMAM dendrimers into macroscopic aggregates with a microribbon structure similar to that of amelogenin

Soft Matter ◽  
2013 ◽  
Vol 9 (31) ◽  
pp. 7553 ◽  
Author(s):  
Jiaojiao Yang ◽  
Shuqin Cao ◽  
Jiahui Li ◽  
Jianyu Xin ◽  
Xingyu Chen ◽  
...  
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers

scholarly journals Controlling the number of dendrimers in dendrimicelle nanoconjugates from 1 to more than 100

Soft Matter ◽  
2014 ◽  
Vol 10 (37) ◽  
pp. 7337-7345 ◽  
Author(s):  
Junyou Wang ◽  
Ilja K. Voets ◽  
Remco Fokkink ◽  
Jasper van der Gucht ◽  
Aldrik H. Velders
Keyword(s):  
Self Assembly ◽  
Diblock Copolymers ◽  
Pamam Dendrimers

The study presents a facile strategy to controllably build up dendrimicelles by self-assembly of anionic PAMAM dendrimers with cationic–neutral diblock copolymers.

DNA Assisted Self-Assembly of PAMAM Dendrimers

2014 ◽  
Vol 118 (40) ◽  
pp. 11805-11815 ◽  
Author(s):  
Taraknath Mandal ◽  
Mattaparthi Venkata Satish Kumar ◽  
Prabal K. Maiti
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers

scholarly journals DNA Condensation Triggered by the Synergistic Self-Assembly of Tetraphenylethylene-Viologen Aggregates and CT-DNA

2021 ◽  
Vol 9 ◽  
Author(s):  
Sajena Kanangat Saraswathi ◽  
Varsha Karunakaran ◽  
Kaustabh Kumar Maiti ◽  
Joshy Joseph
Keyword(s):  
Self Assembly ◽  
Fluorescence Emission ◽  
Dna Condensation ◽  
Condensation Process ◽  
Cellular Delivery ◽  
Groove Binding ◽  
Binding Interaction ◽  
Morphological Studies ◽  
Condensing Agents ◽  
Ct Dna

Development of small organic chromophores as DNA condensing agents, which explore supramolecular interactions and absorbance or fluorescence-based tracking of condensation and gene delivery processes, is in the initial stages. Herein, we report the synthesis and electrostatic/groove binding interaction–directed synergistic self-assembly of the aggregates of two viologen-functionalized tetraphenylethylene (TPE-V) molecules with CT-DNA and subsequent concentration-dependent DNA condensation process. TPE-V molecules differ in their chemical structure according to the number of viologen units. Photophysical and morphological studies have revealed the interaction of the aggregates of TPE-V in Tris buffer with CT-DNA, which transforms the fibrous network structure of CT-DNA to partially condensed beads-on-a-string-like arrangement with TPE-V aggregates as beads via electrostatic and groove binding interactions. Upon further increasing the concentration of TPE-V, the “beads-on-a-string”-type assembly of TPE-V/CT-DNA complex changes to completely condensed compact structures with 40–50 nm in diameter through the effective charge neutralization process. Enhancement in the melting temperature of CT-DNA, quenching of the fluorescence emission of ethidium bromide/CT-DNA complex, and the formation of induced CD signal in the presence of TPE-V molecules support the observed morphological changes and thereby verify the DNA condensation abilities of TPE-V molecules. Decrease in the hydrodynamic size, increase in the zeta potential value with the addition of TPE-V molecules to CT-DNA, failure of TPE-V/cucurbit(8)uril complex to condense CT-DNA, and the enhanced DNA condensation ability of TPE-V2 with two viologen units compared to TPE-V1 with a single viologen unit confirm the importance of positively charged viologen units in the DNA condensation process. Initial cytotoxicity analysis on A549 cancer and WI-38 normal cells revealed that these DNA condensing agents are non-toxic in nature and hence could be utilized in further cellular delivery studies.

Assessment of protective effect of polycation on plasmid DNA attached on the metal surface by SPR

e-Polymers ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Junbo Bao ◽  
Cunxian Song
Keyword(s):  
Molecular Weight ◽  
Protective Effect ◽  
Metal Surface ◽  
Plasmid Dna ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Pamam Dendrimers ◽  
X Ray ◽  
Metal Implants

AbstractSurface modification of the metal is a critical procedure for gene therapy on the metal implants, such as stent and artificial joint. Polycations can condense with DNA by self-assembly, form polyplexes spontaneously as a result of electrostatic interactions and protect DNA from degradation by DNase. The ability of chitosan, polyethyleneimine (PEI), and poly(amidoamine) (PAMAM) dendrimers to condense plasmid DNA(pDNA) was determined by electrophoresis, X-ray photoelectron spectroscopy (XPS) and surface plasmon resonance (SPR). Polycation’s attachment of pDNA to the metal surface was confirmed by XPS which showed no phosphorus peaks on the interface showing the protective effect on pDNA from DNase degradation. Polycations with higher molecular weight or hyperbranched or dendrimer structures can fulfill many of the requirements for effective gene protection from DNase degradation. Chitosan with higher molecular weight (>= 200 kDa) has superior efficiency to protect pDNA against DNase degradation on the surface of the gold chip. Hyperbranched PEIs and PAMAM dendrimers, even with lower molecular weight (for example, 20kDa for PEI, 50kDa for G5) can protect pDNA against DNase degradation. The results of this study present a platform for further optimization studies of polycation-based gene delivery systems.

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