Transmission Electron Microscopy and Time Resolved Optical Spectroscopy Study of the Electronic and Structural Interactions of ZnO Nanorods with Bovine Serum Albumin

2013 ◽  
Vol 117 (33) ◽  
pp. 9683-9689 ◽  
Author(s):  
M. Klaumünzer ◽  
U. Weichsel ◽  
M. Mačković ◽  
E. Spiecker ◽  
W. Peukert ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Zno Nanorods ◽  
Spectroscopy Study ◽  
Time Resolved ◽  
Transmission Electron ◽  
Structural Interactions

scholarly journals Bovine Serum Albumin and Chitosan Coated Silver Nanoparticles and Its Antimicrobial Activity against Oral and Nonoral Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
León Francisco Espinosa-Cristóbal ◽  
Gabriel Alejandro Martínez-Castañón ◽  
Juan Pablo Loyola-Rodríguez ◽  
Nereyda Niño-Martínez ◽  
Facundo Ruiz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Drug Resistance ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Antimicrobial Agents ◽  
Reduction Method ◽  
Gram Positive Bacteria ◽  
Transmission Electron

Antimicrobial agents have been developed for drug-resistance infections, which have been rapidly increasing; however, the control of involved microorganisms is still a challenge. In this work, SNP with bovine serum albumin (BSA) and chitosan (CS) coatings were prepared with an aqueous reduction method, characterized using dispersion light scattering, transmission electron microscopy, and thermal analysis. Antibacterial activity was tested on seven oral and nonoral bacteria by microdilution test and scanning electron microscopy. Six different sizes and shapes of coated SNP were prepared and used. Characterization revealed narrow size and good distribution of particles, spherical and pseudospherical shapes, and the presence of coatings on the SNP surfaces. All samples showed antimicrobial activity, although smaller sizes and CS samples had the best inhibition effects. The highest microbial resistance was shown by Gram-positive bacteria. Although coated SNP action depends on particular bacterium, BSA and CS coated SNP could be used for drug-resistance infections.

Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays

2021 ◽  
Vol 21 (11) ◽  
pp. 5493-5498
Author(s):  
Paulo Emilio Feuser ◽  
Camila Guindani ◽  
Jonathann Correa Possato ◽  
Jaqueline Pereira Guessi ◽  
Arthur Poester Cordeiro ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Methyl Methacrylate ◽  
Bovine Serum ◽  
Protein Quantification ◽  
Elisa Assay ◽  
Poly Methyl Methacrylate ◽  
Transmission Electron ◽  
Superparamagnetic Properties ◽  
Significant Attention

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.

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