Solvation Effects on Self-Association and Segregation Processes in tert-Butanol–Aprotic Solvent Binary Mixtures

2013 ◽  
Vol 117 (35) ◽  
pp. 10221-10230 ◽  
Author(s):  
A. R. Abdel Hamid ◽  
R. Lefort ◽  
Y. Lechaux ◽  
A. Moréac ◽  
A. Ghoufi ◽  
...  
Keyword(s):  
Binary Mixtures ◽  
Aprotic Solvent ◽  
Solvation Effects ◽  
Tert Butanol ◽  
Self Association

Heterogeneous Biocatalytic Processes of the Low-Temperature Synthesis of Esters: Selection of Organic Solvent

2020 ◽  
Vol 20 (4) ◽  
pp. 313-322
Author(s):  
G. A. Kovalenko ◽  
L. V. Perminova
Keyword(s):  
Low Temperature ◽  
Binary Mixtures ◽  
Organic Solvents ◽  
Diethyl Ether ◽  
Operational Stability ◽  
Thermomyces Lanuginosus ◽  
Esterification Reaction ◽  
Heptanoic Acid ◽  
Tert Butanol ◽  
Heterogeneous Biocatalysts

Processes of the low-temperature enzymatic synthesis of esters in nonaqueous media of organic solvents with participation of heterogeneous biocatalysts synthesized by the adsorption immobilization of a recombinant lipase from Thermomyces lanuginosus (Pichia pastoris producer) on macroporous carbon aerogel were investigated. The esterification reaction was conducted using individual organic solvents of different polarity, such as nonpolar ones (lоgP > 2.5) – hexadecane, hexane and toluene, and polar solvents (lоgP < 1) – diethyl ether, tert-butanol and acetone, as well as their binary mixtures of different composition, for example, a mixture of hexane with diethyl ether having the composition 1 : (1/5÷3, vol. fr.). The effect of the solvent and co-solvent nature on the properties of synthesized biocatalysts, namely the enzymatic activity and operational stability, was studied in the batch esterification of heptanoic acid (C7:0) by alcohols, particularly the polar substrates – ethanol and n-butanol, or nonpolar alcohols – n-octanol and n-hexadecanol. Activity of the synthesized biocatalysts was found to depend to a great extent on polarity of both the solvents used in the reaction and the alcohol substrates. The maximum activity of 400 EA·g–1 was observed in a medium of nonpolar and nonviscous hexane (lоgP = 2.9), whereas the minimum activity of 4 EA·g–1 – in acetone (lоgP = 0.4); esterification of heptanoic acid in the presence of tert-butanol (lоgP = 0.6) virtually did not proceed. The activity of biocatalysts was shown to depend nonmonotonically on the lоgP value of individual solvent. In the binary mixtures of organic solvents, such as hexane/diethyl ether, biocatalytic activity linearly increased with an increase in lоgPmixture; in addition, biocatalysts exhibited the maximum operational stability.

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