Properties of Adsorbed Bovine Serum Albumin and Fibrinogen on Self-Assembled Monolayers

2013 ◽  
Vol 117 (12) ◽  
pp. 6151-6160 ◽  
Author(s):  
Lalit M. Pandey ◽  
Sudip K. Pattanayek ◽  
Didier Delabouglise
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

Morphology Effect on Charge Transport in Doped Bovine Serum Albumin Self-Assembled Monolayers

2017 ◽  
Vol 121 (17) ◽  
pp. 9579-9586 ◽  
Author(s):  
Edith Beilis ◽  
Yonatan Horowitz ◽  
Alon Givon ◽  
Gabor A. Somorjai ◽  
Hagai Cohen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Charge Transport ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Morphology Effect ◽  
Self Assembled

Surface-Induced Conformational Changes in Doped Bovine Serum Albumin Self-Assembled Monolayers

2014 ◽  
Vol 136 (17) ◽  
pp. 6151-6154 ◽  
Author(s):  
Edith Beilis ◽  
Bogdan Belgorodsky ◽  
Ludmila Fadeev ◽  
Hagai Cohen ◽  
Shachar Richter
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

In-Silico Prediction on the MSAMS-Assisted Immobilization of Bovine Serum Albumin on 10MHz Piezoelectric Immunosensors

2019 ◽  
Vol 07 (01n02) ◽  
pp. 1950001 ◽  
Author(s):  
Kaory Barrientos Urdinola ◽  
Paula Andrea Marín Muñoz ◽  
Pedronel Araque Marín ◽  
Marisol Jaramillo Grajales
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Molecular Model ◽  
Self Assembled Monolayers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Gold Substrates ◽  
Assembled Monolayers ◽  
Sensitivity Specificity

The biological sensing interface on the active area of a piezo transducer is responsible for the sensitivity, specificity, reusability, and reproducibility of these devices. Among the approaches used to functionalize piezo transducers, mixed self-assembled monolayers (MSAMs) are one of the most successful, given that they allow the obtaining of semi-crystalline molecular arrays and the arrangement of a bioreceptor on the surface. But, to deploy MSAMs on a substrate effectively, one must optimize and characterize the structural ratio between them and the bioreceptor. In this paper, we developed a molecular model of the interaction between Bovine Serum Albumin (BSA) and MSAMs-functionalized gold substrates. First, we evaluated the conditions for the functionalization of the substrates and found that a 50:1 16-mercaptohexadecaonic acid (MHDA) to 11 mercapto-1-undecanol (MUA) ratio produced the best features on the surface. We also evaluated the specific conditions to immobilize BSA on MSAMs (using the afore-established ratio) via Atomic Force Microscopy (AFM), and then on a 10[Formula: see text]MHz quartz crystal microbalance (QCM), and with the data obtained we concluded that a structural ratio of 0.005 (MSAM/BSA) is obtained when 1[Formula: see text][Formula: see text]M MHDA and 200[Formula: see text][Formula: see text]g/mL BSA were used, provided the most suitable conditions for the functionalization of a piezo transducer.

Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells

2016 ◽  
Vol 8 (15) ◽  
pp. 9629-9634 ◽  
Author(s):  
Qiaoyu Yang ◽  
Zhongju Ye ◽  
Meile Zhong ◽  
Bo Chen ◽  
Jian Chen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Living Cells ◽  
Ph Measurement ◽  
Self Assembled

Neutron and X-ray Reflectivity Studies of Human Serum Albumin Adsorption onto Functionalized Surfaces of Self-Assembled Monolayers

1997 ◽  
Vol 13 (5) ◽  
pp. 635-639 ◽  
Author(s):  
S. Petrash ◽  
A. Liebmann-Vinson ◽  
M.D. Foster ◽  
L.M. Lander ◽  
W.J. Brittain ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembled Monolayers ◽  
X Ray ◽  
Functionalized Surfaces ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Albumin Adsorption

scholarly journals Self-assembled bovine serum albumin nanoparticles as pesticide delivery vectors for controlling trunk-boring pests

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Chenyu Su ◽  
Shanshan Liu ◽  
Shenghan Cao ◽  
Shuyan Yin ◽  
Chenggang Zhou ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Self Assembly ◽  
Bovine Serum ◽  
Fluorescein Isothiocyanate ◽  
Effective Control ◽  
Transport Efficiency ◽  
Dead Trees ◽  
Self Assembled ◽  
And Control

Abstract Background Trunk-boring pests (TBPs) are an important type of forest pest, TBPs not only feed on the branches and trunks of trees, but also spread quarantine diseases in forests. However, because the larvae of TBPs live inside the trunk and are well concealed, prevention and control are difficult. The lack of effective control methods leads to the death of many trees in forests. In this study, a novel nanopesticide featuring high bioactivity and slow-release properties was developed to control TBPs. Thiacloprid (THI), which is commonly used to control Coleoptera species, was used as a model pesticide. Results The oleophobic properties of bovine serum albumin (BSA) were exploited to encapsulate the hydrophobic pesticide THI by self-assembly, and the size of the obtained nanoparticles, THI@BSA·NPs, was approximately 23 nm. The loading efficiency reached 70.4%, and THI@BSA·NPs could be released continuously for over 15 days, with the cumulative release reaching 93.5%. The fluorescein isothiocyanate (FITC)-labeled nanoparticles were evenly distributed in the digestive tract and body surface of a typical TBPs, M. alternatus, and the stomach and contact toxicities increased by 33.7% and 25.9%, respectively, compared with those of free THI. Furthermore, the results showed that the transport efficiency of THI@BSA·NPs was highest at a concentration of 50 μg/mL, and the THI@BSA·NPs content in the trunk, from to lower to higher layers, was 8.8, 8.2, 7.6, and 5.8 μg/g. At the same time, THI@BSA·NPs also exhibited high transport efficiency in dead trees. Conclusion The transport efficiency and toxicity of the active ingredients are the key factors for the control of TBPs. This work provided idea for the application of biological delivery system encapsulated hydrophobic pesticides. The novel self-assembled THI@BSA·NPs have promising potential for sustainable control of TBPs.

scholarly journals Self-Assembled Bovine Serum Albumin Nanoparticles as Pesticide Delivery Vectors for Controlling Trunk-Boring Pests

2020 ◽  
Author(s):  
Chenyu Su ◽  
Shanshan Liu ◽  
Shenghan Cao ◽  
Shuyan Yin ◽  
Chenggang Zhou ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Self Assembly ◽  
Bovine Serum ◽  
Fluorescein Isothiocyanate ◽  
Effective Control ◽  
Transport Efficiency ◽  
Dead Trees ◽  
Self Assembled ◽  
And Control

Abstract Trunk-boring pests (TBPs) are an important type of forest pest, TBPs not only feed on the branches and trunks of trees, but also spread quarantine diseases in forests. However, because the larvae of TBPs live inside the trunk and are well concealed, prevention and control are difficult. The lack of effective control methods leads to the death of many trees in forests. In this study, a novel nanopesticide featuring high bioactivity and slow-release properties was developed to control TBPs. Thiacloprid (THI), which is commonly used to control Coleoptera species, was used as a model pesticide. The oleophobic properties of bovine serum albumin (BSA) were exploited to encapsulate the hydrophobic pesticide THI by self-assembly, and the size of the obtained nanoparticles, THI@BSA·NPs, was approximately 23 nm. The loading efficiency reached 70.4%, and THI@BSA·NPs could be released continuously for over 15 d, with the cumulative release reaching 93.5%. The fluorescein isothiocyanate (FITC)-labeled nanoparticles were evenly distributed in the digestive tract and body surface of a typical TBP, M. alternatus , and the stomach and contact toxicities increased by 33.7% and 25.9%,respectively, compared with those of free THI. Furthermore, the results showed that the transport efficiency of THI@BSA·NPs was highest at a concentration of 50 μg/mL, and the THI@BSA·NPs content in the trunk, from to lower to higher layers, was 8.8, 8.2, 7.6, and 5.8 μg/g. At the same time, THI@BSA·NPs also exhibited high transport efficiency in dead trees. The results suggested that these novel self-assembled THI@BSA·NPs have promising potential for sustainable control of TBPs.

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