Surface-Induced Conformational Changes in Doped Bovine Serum Albumin Self-Assembled Monolayers

2014 ◽  
Vol 136 (17) ◽  
pp. 6151-6154 ◽  
Author(s):  
Edith Beilis ◽  
Bogdan Belgorodsky ◽  
Ludmila Fadeev ◽  
Hagai Cohen ◽  
Shachar Richter
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

Morphology Effect on Charge Transport in Doped Bovine Serum Albumin Self-Assembled Monolayers

2017 ◽  
Vol 121 (17) ◽  
pp. 9579-9586 ◽  
Author(s):  
Edith Beilis ◽  
Yonatan Horowitz ◽  
Alon Givon ◽  
Gabor A. Somorjai ◽  
Hagai Cohen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Charge Transport ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Morphology Effect ◽  
Self Assembled

Properties of Adsorbed Bovine Serum Albumin and Fibrinogen on Self-Assembled Monolayers

2013 ◽  
Vol 117 (12) ◽  
pp. 6151-6160 ◽  
Author(s):  
Lalit M. Pandey ◽  
Sudip K. Pattanayek ◽  
Didier Delabouglise
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

In-Silico Prediction on the MSAMS-Assisted Immobilization of Bovine Serum Albumin on 10MHz Piezoelectric Immunosensors

2019 ◽  
Vol 07 (01n02) ◽  
pp. 1950001 ◽  
Author(s):  
Kaory Barrientos Urdinola ◽  
Paula Andrea Marín Muñoz ◽  
Pedronel Araque Marín ◽  
Marisol Jaramillo Grajales
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Molecular Model ◽  
Self Assembled Monolayers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Gold Substrates ◽  
Assembled Monolayers ◽  
Sensitivity Specificity

The biological sensing interface on the active area of a piezo transducer is responsible for the sensitivity, specificity, reusability, and reproducibility of these devices. Among the approaches used to functionalize piezo transducers, mixed self-assembled monolayers (MSAMs) are one of the most successful, given that they allow the obtaining of semi-crystalline molecular arrays and the arrangement of a bioreceptor on the surface. But, to deploy MSAMs on a substrate effectively, one must optimize and characterize the structural ratio between them and the bioreceptor. In this paper, we developed a molecular model of the interaction between Bovine Serum Albumin (BSA) and MSAMs-functionalized gold substrates. First, we evaluated the conditions for the functionalization of the substrates and found that a 50:1 16-mercaptohexadecaonic acid (MHDA) to 11 mercapto-1-undecanol (MUA) ratio produced the best features on the surface. We also evaluated the specific conditions to immobilize BSA on MSAMs (using the afore-established ratio) via Atomic Force Microscopy (AFM), and then on a 10[Formula: see text]MHz quartz crystal microbalance (QCM), and with the data obtained we concluded that a structural ratio of 0.005 (MSAM/BSA) is obtained when 1[Formula: see text][Formula: see text]M MHDA and 200[Formula: see text][Formula: see text]g/mL BSA were used, provided the most suitable conditions for the functionalization of a piezo transducer.

Self-Assembled Fluorescent Bovine Serum Albumin Nanoprobes for Ratiometric pH Measurement inside Living Cells

2016 ◽  
Vol 8 (15) ◽  
pp. 9629-9634 ◽  
Author(s):  
Qiaoyu Yang ◽  
Zhongju Ye ◽  
Meile Zhong ◽  
Bo Chen ◽  
Jian Chen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Living Cells ◽  
Ph Measurement ◽  
Self Assembled

scholarly journals Thermally induced conformational changes and protein–protein interactions of bovine serum albumin in aqueous solution under different pH and ionic strengths as revealed by SAXS measurements

2017 ◽  
Vol 19 (26) ◽  
pp. 17143-17155 ◽  
Author(s):  
Dmitry Molodenskiy ◽  
Evgeny Shirshin ◽  
Tatiana Tikhonova ◽  
Andrey Gruzinov ◽  
Georgy Peters ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Changes ◽  
Interaction Potential ◽  
Protein Interactions ◽  
Bovine Serum ◽  
Protein Protein Interactions ◽  
Thermally Induced ◽  
Before And After

Temperature-induced oligomerization of albumin before and after protein melting was studied using SAXS and interpreted in terms of interaction potential.

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